Miyoko Massago1,2, Gerson Zanusso Junior3,4, Elaine Schultz Dworak4, Elisama Loubak da Silva4, Alexandre Tadachi Morey5, Mônica Lúcia Gomes4,6, Max Jean de Ornelas Toledo7,4,6. 1. Interdisciplinary Dynamic Museum, State University of Maringa, Av. Colombo 5790, Bloco I-90, Zona 7, Maringa, Paraná, 87020-900, Brazil. mi_massago@hotmail.com. 2. Post-Graduate Program of Biological Sciences, Biological Sciences Center at State University of Maringa, Maringa, Paraná, Brazil. mi_massago@hotmail.com. 3. Blood Center at State University of Maringa, Maringa, Paraná, Brazil. 4. Post-Graduate Program of Health Sciences, Health Sciences Center at State University of Maringa, Maringa, Paraná, Brazil. 5. Federal Institute of Education, Science and Technology of Rio Grande do Sul, Canoas, Rio Grande do Sul, Brazil. 6. Department of Basic Health Sciences, Health Sciences Center at State University of Maringa, Maringa, Paraná, Brazil. 7. Post-Graduate Program of Biological Sciences, Biological Sciences Center at State University of Maringa, Maringa, Paraná, Brazil.
Abstract
PURPOSE: Our goal was to analyze the outcome of infection and response to benznidazole (BZ) treatment in mice intragastrically inoculated with trypomastigotes forms of Trypanosoma cruzi from different origins. METHODS: Twenty-four Swiss mice were divided in two groups and inoculated, by gavage, with 1 × 104 blood trypomastigotes (BT) or insect-derived metacyclic trypomastigotes (IT) of AM14 strain (T. cruzi IV). Half of the animals of each group were treated with BZ (TBZ), from 10 to 30th days after the inoculation, and the other constituted the untreated control groups (NT). After the etiological treatment, all mice were immunosuppressed with cyclophosphamide for three weeks. Parasitological and molecular parameters, infectivity, cumulative mortality, and reactivation post-immunosuppression rates were obtained. RESULTS: Animals inoculated with BT showed lower pre-patent period and early day of the maximum parasitemia, as well as a higher maximum peak of parasitemia than the IT animals. However, both, BT and IT animals, did not respond to BZ treatment (0.0% of cure). CONCLUSION: We conclude that the infective form influences in the outcome of infection, but not the response to the etiological treatment in mice intragastrically infected with the T. cruzi IV strain studied.
PURPOSE: Our goal was to analyze the outcome of infection and response to benznidazole (BZ) treatment in mice intragastrically inoculated with trypomastigotes forms of Trypanosoma cruzi from different origins. METHODS: Twenty-four Swiss mice were divided in two groups and inoculated, by gavage, with 1 × 104 blood trypomastigotes (BT) or insect-derived metacyclic trypomastigotes (IT) of AM14 strain (T. cruzi IV). Half of the animals of each group were treated with BZ (TBZ), from 10 to 30th days after the inoculation, and the other constituted the untreated control groups (NT). After the etiological treatment, all mice were immunosuppressed with cyclophosphamide for three weeks. Parasitological and molecular parameters, infectivity, cumulative mortality, and reactivation post-immunosuppression rates were obtained. RESULTS: Animals inoculated with BT showed lower pre-patent period and early day of the maximum parasitemia, as well as a higher maximum peak of parasitemia than the IT animals. However, both, BT and IT animals, did not respond to BZ treatment (0.0% of cure). CONCLUSION: We conclude that the infective form influences in the outcome of infection, but not the response to the etiological treatment in mice intragastrically infected with the T. cruzi IV strain studied.
Authors: Ana Paula Gruendling; Miyoko Massago; Ana Paula M Teston; Wuelton M Monteiro; Edilson N Kaneshima; Silvana M Araújo; Mônica L Gomes; Maria das Graças V Barbosa; Max Jean O Toledo Journal: Am J Trop Med Hyg Date: 2015-05-04 Impact factor: 2.345
Authors: Daniela M Santos; Tassiane A F Martins; Ivo S Caldas; Lívis F Diniz; George L L Machado-Coelho; Cláudia M Carneiro; Riva de P Oliveira; André Talvani; Marta Lana; Maria T Bahia Journal: Acta Trop Date: 2009-10-23 Impact factor: 3.112