Keisuke Kuhara1,2, Takao Kitagawa1, Byron Baron3, Kazuhiro Tokuda4, Kazuhiko Sakamoto5, Hiroaki Nagano5, Kazuyuki Nakamura6, Masanobu Kobayashi1, Hiroki Nagayasu2, Yasuhiro Kuramitsu7. 1. Advanced Research Promotion Centre, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. 2. Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. 3. Centre for Molecular Medicine and Biobanking, University of Malta, Msida, Malta. 4. Graduate School of Health and Welfare, Yamaguchi Prefectural University, Yamaguchi, Japan. 5. Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan. 6. Centre of Clinical Laboratories in Tokuyama Medical Association Hospital, Shunan, Japan. 7. Advanced Research Promotion Centre, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan; climates@hoku-iryo-u.ac.jp.
Abstract
BACKGROUND/AIM: Cancer is the most fatal disease worldwide whose most lethal characteristics are invasion and metastasis. Hepatocellular carcinoma (HCC) is one of the most fatal cancers worldwide. HCC often shows encapsulation, which is related to better prognosis. In this study, proteomic analysis of HCC tissues with and without encapsulation was performed, in order to elucidate the factors which play important roles in encapsulation. MATERIALS AND METHODS: Five HCC tissues surrounded by a capsule and five HCC tissues which broke the capsule were obtained from patients diagnosed with HCC who underwent surgical liver resection. Protein samples from these tissues were separated by two-dimensional gel electrophoresis (2-DE), and the protein spots whose expression was different between encapsulated and non-encapsulated HCC tissues were identified through gel imaging analysis software. The selected protein spots were analyzed and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: Two-DE analysis showed 14 spots whose expression was different between encapsulated and non-encapsulated HCC tissues. Of these, 9 were up-regulated and 5 were down-regulated in HCC tissues without encapsulation. The validation by Western blot confirmed that leucine aminopeptidase 3 (LAP3) and phosphoenolpyruvate carboxykinase mitochondrial (PCK2) were up-regulated significantly in HCC tissues with a capsule, compared to HCC tissues that broke the capsule. CONCLUSION: These findings suggest that LAP3 and PCK2 could be factors responsible for the maintenance of encapsulation in HCC tissues. Copyright
BACKGROUND/AIM: Cancer is the most fatal disease worldwide whose most lethal characteristics are invasion and metastasis. Hepatocellular carcinoma (HCC) is one of the most fatal cancers worldwide. HCC often shows encapsulation, which is related to better prognosis. In this study, proteomic analysis of HCC tissues with and without encapsulation was performed, in order to elucidate the factors which play important roles in encapsulation. MATERIALS AND METHODS: Five HCC tissues surrounded by a capsule and five HCC tissues which broke the capsule were obtained from patients diagnosed with HCC who underwent surgical liver resection. Protein samples from these tissues were separated by two-dimensional gel electrophoresis (2-DE), and the protein spots whose expression was different between encapsulated and non-encapsulated HCC tissues were identified through gel imaging analysis software. The selected protein spots were analyzed and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: Two-DE analysis showed 14 spots whose expression was different between encapsulated and non-encapsulated HCC tissues. Of these, 9 were up-regulated and 5 were down-regulated in HCC tissues without encapsulation. The validation by Western blot confirmed that leucine aminopeptidase 3 (LAP3) and phosphoenolpyruvate carboxykinase mitochondrial (PCK2) were up-regulated significantly in HCC tissues with a capsule, compared to HCC tissues that broke the capsule. CONCLUSION: These findings suggest that LAP3 and PCK2 could be factors responsible for the maintenance of encapsulation in HCC tissues. Copyright
Authors: L P Ooi; D H Crawford; D C Gotley; A D Clouston; R W Strong; G C Gobe; J W Halliday; K R Bridle; G A Ramm Journal: J Hepatol Date: 1997-04 Impact factor: 25.083
Authors: Raghothama Chaerkady; H C Harsha; Anuradha Nalli; Marjan Gucek; Perumal Vivekanandan; Javed Akhtar; Robert N Cole; Jessica Simmers; Richard D Schulick; Sujay Singh; Michael Torbenson; Akhilesh Pandey; Paul J Thuluvath Journal: J Proteome Res Date: 2008-08-21 Impact factor: 4.466
Authors: Harry J Layfield; Harry F Williams; Divyashree Ravishankar; Amita Mehmi; Medha Sonavane; Anika Salim; Rajendran Vaiyapuri; Karthik Lakshminarayanan; Thomas M Vallance; Andrew B Bicknell; Steven A Trim; Ketan Patel; Sakthivel Vaiyapuri Journal: Toxins (Basel) Date: 2020-05-09 Impact factor: 4.546