Pseudomonas stutzeri GF2 augmented the denitrification of low carbon to nitrogen ratio: Possibility for sewage wastewater treatment.

A denitrifying strain with high efficiency at low carbon to nitrogen (C/N) ratio of 2.0 was isolated and characterized. It belongs to the genus Pseudomonas. Scanning electron microscopy (SEM) showed that GF2 was rod-shaped. The nitrate removal efficiency reached up to 92.41% (1.85 mg L-1 h-1) with the C/N ratio of 2.0 and the nitrite accumulation eventually decreased to 0.88 mg L-1. By response surface method (RSM) method, three reaction conditions of strain GF2 were optimized, including pH, C/N ratio, and nitrate concentration. Nitrogen balance and gas detection revealed that 88.03% of nitrogen was removed in gaseous form (included 98.80% nitrogen gas), which confirmed its efficient denitrification ability and pathway. 3D fluorescence spectrum (3D-EEM) manifested that in the absence of organic matter, strain GF2 can utilize extracellular polymeric substance (EPS) as carbon source for efficient denitrification. This research strived to provide new research ideas for low C/N ratio sewage treatment.