Clinical Analysis of Metagenomic Next-Generation Sequencing Confirmed Chlamydia psittaci Pneumonia: A Case Series and Literature Review.

INTRODUCTION: Chlamydia psittaci infection is a zoonotic infectious disease, which mainly inhaled through the lungs when exposed to the secretions of poultry that carry pathogenic bacteria. The traditional respiratory specimens or serological antibody testing is slow, and the false-negative rate is high. Metagenomic next-generation sequencing (mNGS) gives a promising rapid diagnosis tool.
METHODS: We retrospectively summarized the clinical characteristics of five C. psittaci pneumonia patients diagnosed by mNGS, conducted a literature review summarizing the clinical characteristics of patients with C. psittaci pneumonia reported since 2010.
RESULTS: Five C. psittaci pneumonia patients confirmed by mNGS aged from 36 to 66 years with three males. About 60% of patients had a history of contact with avian or poultry. All patients had a high fever over 38.5 °C, cough, hypodynamia, hypoxemia, and dyspnea on admission. Two patients had invasive ventilator support and extracorporeal membrane oxygenation support. Inflammatory index levels on admission and follow-up were all higher than normal values. Doxycycline or moxifloxacin and their combination therapy were used in patients. Four patients improved and were discharged, and one patient died due to multiple organ failures and disseminated intravascular coagulation. We summarized 19 articles including 69 C. psittaci pneumonia patients and patients in 11 publications were identified by mNGS, and most patients are treated with tetracycline and quinolone with good outcomes.
CONCLUSION: mNGS is a promising rapid diagnosis tool, which may increase the detection rate and shorten the diagnosis time of C. psittaci pneumonia. Further case-control studies are needed to confirm.

Introduction

Chlamydia psittaci pneumonia is caused by Chlamydia psittaci (C. psittaci), which can lead to severe pneumonia, adult respiratory distress syndrome, and even death.1 According to the sequence difference of C. psittaci outer membrane protein A gene (ompA), it can be divided into 10 genotypes, namely A-G, WC, E/B, and M56, among which genotype A is the main genotype that causes human infection.2 About 70% of respiratory tract infections caused by C. psittaci are asymptomatic or only with mild symptoms, but 30% of them are severe respiratory illnesses such as community-acquired pneumonia with atypical symptoms, bronchitis, and upper respiratory tract infections.3 Contacting with birds or poultry is regarded as the main risk factor for psittacosis.1 The clinical symptoms of C. psittaci infection are quite different and lack specificity, which ranges in severity from mild to severe. Since the clinical manifestations of C. psittaci are similar to influenza symptoms, and the extrapulmonary manifestations are similar to Legionella, it needs to be differentiated from influenza and Legionella.4 Recent publications also reported the co-infection of SARS-CoV-2 with Chlamydia,5–7 which makes infectious diseases more complex.

The culture of C. psittaci from respiratory secretions in special media is possible but difficult, and it is mainly performed in specialized laboratories only because of the high infectivity of this pathogen. Specific diagnostic testing is serological, which is regarded as the gold standard for C. psittaci pneumonia. Moreover, the micro-immunofluorescence test (MIF) is the most accurate serologic test for C. psittaci pneumonia but is also performed only in specialized laboratories. Polymerase chain reaction assay (PCR) is used to confirm the strong clinical suspicion of a possible diagnosis of psittacosis especially, to distinguish it from other chlamydial species.8 And complement fixation (CF) is also an acceptable diagnostic method. Detection methods for C. psittaci, such as PCR, CF, and MIF are not routinely available in most hospitals in China. Because of its non-specific symptoms and the limitations of traditional tests, C. psittaci pneumonia is easily underdiagnosed and misdiagnosed.9

Metagenomic next-generation sequencing (mNGS) can quickly and accurately identify potential pathogens, whether they are bacteria, fungi, viruses, or parasites.10 It is increasingly used for the diagnosis of infectious diseases, especially when traditional diagnostic methods have limitations.11 Studies have shown that mNGS is the most promising comprehensive diagnosis method for infection, especially for severe pneumonia.12 Recently, several studies have reported the application of mNGS in diagnosing C. psittaci pneumonia, two case reports describing 5 and 9 cases of C. psittaci diagnosed by mNGS13,14.

We retrospectively summarized the clinical characteristics of five C. psittaci pneumonia patients diagnosed by mNGS in our hospital. Besides, we conducted a literature review of patients with C. psittaci pneumonia reported since 2010, with the attention to summarize the diagnostic methods and anti-infective drugs. We also summarized the clinical outcome and history of exposure to avian or poultry of these infection patients to provide a reference for future C. psittaci pneumonia infection patients’ diagnosis and treatment. We present the following article following the CARE reporting checklist.

Case Presentations

Patients’ Information

We carried out a retrospective case series analysis of five patients admitted to the Second Xiangya Hospital of Central South University since 2018. We collected the clinical data of all patients confirmed to have C. psittaci pneumonia. Sex, age, clinical examination indexes such as procalcitonin (PCT), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), comprehensive computed tomography (CT), and arterial blood gas analysis were extracted from electronic medical records. The treatment of antibiotics, outcomes, and any relevant follow-up data were also collected.

The Ethics Committees of the Second Xiangya Hospital of Central South University (LYF-2020021) approved this study. Informed consent was obtained from patients and guardians. This study was carried out by the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. All data were anonymized before analysis.

As the symptoms of the patients were sudden, some patients had no sputum and their blood oxygen saturation was low, we did not perform bronchoscopy for them on admission. The blood was used for performing mNGS testing on admission for all five patients, and only case 5 performed bronchoalveolar lavage fluid (BAFL) mNGS testing on admission. All five patients were positive for C. psittaci DNA fragments in the blood mNGS test. Moreover, BAFL mNGS of case 5 result showed Klebsiella DNA fragments. The median duration from admission to the mNGS pathogenic diagnosis of the 5 cases was 3 days (range from 2 to 4). The median of mNGS detection sequence number of C. psittaci was 217 (range from 175 to 289). No pathogenic microorganisms were found in the sputum, BAFL, and blood culture of the patient after admission to our hospital. We also conducted serological verification and PCR for five cases after an atypical pathogen was identified by mNGS. Serological detection of antibody to C. pneumoniae was positive only in case 2 and the PCR of C. pneumoniae was negative in all patients. While the serological detection of antibody, PCR, MIF, and CF of C. psittaci cannot be performed in our hospital.

There were three male and two female patients with a median age of 51 years (range from 36 to 66). 60% of patients had type 2 diabetes. Three of five patients had a history of contact with avian or poultry. All patients had a high fever over 38.5 °C, cough, hypodynamia, hypoxemia, and dyspnea. Two of five patients had a headache. In the course of treatment, two patients had invasive ventilator support and Extracorporeal Membrane Oxygenation (ECOM) support. The medium APACHE II was 17.6 (range from 8 to 22). Days from illness to respiratory failure were 4.8 (2–8) days.

Laboratory Test

mNGS was conducted using the following operational steps according to the company’s operating procedures (The Beijing Genomics Institute, Beijing, China). Briefly, clinical samples (blood or BALF)) were collected by following the standards of aseptic processing procedures. Nucleic acid extraction was conducted and the extracted DNA was subjected to processes of interruption, end repair, library construction, and sequencing. The mapped data were processed for advanced data analysis. Lists of suspected pathogenic microorganisms were produced, which included the numbers of strictly mapped reads, coverage rates, and depth. The clinical diagnosis was determined by considering all the clinical manifestations, possible pathogens identified by mNGS, and other laboratory tests together. On admission and during the hospital, inflammatory markers such as PCT, CRP, ESR, kidney, and liver function index, CT, and X-ray data were detected according to the patients’ condition.

Laboratory inspection parameters of the five patients on admission were shown in Table 2. Four patients had lower hemoglobin and the levels were 108.6 (range 68–148) g/L. The levels of CRP, PCT, and ESR on admission were all higher than normal values. Oxygen partial pressure and partial pressure of carbon dioxide of all patients were significantly lower than normal values.

Table 2

Laboratory Inspections Parameters of the Five Patients on Admission

Inspection Items	Case 1	Case 2	Case 3	Case 4	Case5	Reference Value
PH	7.504	7.47	7.52	7.4	7.53	7.35–7.45
Oxygen partial pressure (mmHg)	64.6	42	53.1	59	58	80–100
Partial pressure of carbon dioxide (mmHg)	28.8	29	26.7	27	29	35–45
White blood cell count (x 109/L)	16.88	8.43	13.56	5.66	2.1	3.5–9.5
Hemoglobin (g/L)	128	127	68	148	72	130–175
Platelets (x 109/L)	183	129	325	83	100	125–350
C reactive protein (mg/L)	423	109.12	434	179.5	191.59	0–8
Procalcitonin (ng/mL)	18.17	8.1	13.89	58.5	2.75	0–0.05
Erythrocyte sedimentation rate (mm/h)	68	100	–	82	32	0–15
Alanine aminotransferase (IU/L)	146.7	91.7	237	33.4	79.2	9–50
Aspartate aminotransferase (IU/L)	139	115.8	587.6	60	246	15–40
Total bilirubin (umol/L)	20.4	8.9	16.3	12.6	5.8	3.4–17.1
Serum creatinine (umol/L)	71.4	79.7	48.6	338	53.5	44–133
Outcome	Survival	Survival	Survival	Survival	Death

After admission, follow-up laboratory testing results showed that the levels of inflammatory markers including WBC, percentage of neutrophils, CRP, PCT, ESR were all higher than normal values (Table 1). Moreover, the values of CK, lactate dehydrogenase (LDH), ALT, and AST were all higher than normal values in all five patients. Three patients had hypokalemia and the potassium levels were range from 2.7 to 3.3 mmol/L.

Table 1

Clinical Characteristics of the Five C. Psittaci Pneumonia Cases

Characteristics	Abnormal Patients/Total Patients, n (%)	Median Value (Range)
Demographics
Age, median (range, years)		51 (36–66)
History of exposure to avian or poultry	3/5 (60)
Underlying disease	3/5 (60)
Clinical manifestations
Fever > 38.5 °C	5/5 (100)	39.1 (38.7–40.5)
Cough, hypodynamia, dyspnea	5/5 (100)
Headache	2/5 (40)
Hypoxemia	5/5 (100)
Invasive ventilator support	2/5 (40)
ECOM support	2/5 (40)
APACHE II		17.6 (8–22)
Days from illness to respiratory failure		4.8 (2–8)
NGS detection sequence number		217 (175–289)
Laboratory testing
WBC (normal 4–10, × 109/L)	4/5 (80)	14.35 (10.4–20.08)
Neutrophil ratio (normal 45–75%)	5/5 (100)	93.03 (80.5–97.7)
CRP (normal 0–8 mg/L)	5/5 (100)	129.83 (16.8–423)
PCT (normal 0–0.05 ng/mL)	5/5 (100)	10.12 (0.058–100)
ESR (normal 0–15mm/h)	5/5 (100)	65.64 (25–100)
CK (normal 30–135 U/L)	5/5 (100)	2261.18 (14–8842.2)
LDH (normal 109–245 U/L)	5/5 (100)	737.96 (252.9–1433.1)
ALT (normal 9–50 U/L)	5/5 (100)	310.17 (65.7–2889.7)
AST (normal 15–40 U/L)	5/5 (100)	1554.11 (40.4–23,553.3)
Hypokalemia (normal 3.5–5.3 mmol/L)	3/5 (80)	3.1 (2.7–3.3)*
Hemoglobin
Treatment
Doxycycline	2/5 (40)
Moxifloxacin	1/5 (20)
Moxifloxacin+Doxycycline	2/5 (40)
The duration from admission to diagnosis (days)		3 (2–4)
Treatment result
Survive	4/5 (80)
Death	1/5 (20)

Note: *The potassium levels just analyzed the values of hypokalemia patients.

Abbreviations: ECOM, extracorporeal membrane oxygenation; APACHE, The Acute Physiology and Chronic Health Evaluation; CK, creatine kinase; CRP, C-reactive protein; CT, computed tomography; LDH, lactate dehydrogenase; PCT, procalcitonin; ALT, alanine transaminase; AST, aspartate aminotransferase; WBC, white blood cell; ESR, erythrocyte sedimentation rate.

Chest CT and X-ray of patients showed that lungs were exuding consolidation foci, bilateral pectoral effusions, consolidation. After treatment, the image of four patients improved compared with those on admission, and the patients’ lung exudation, consolidation, and bilateral pleural effusion were less than before (Figures 1 and 2).

Figure 1

Chest computed tomography (CT) scans of a 66-year-old man with C. psittaci pneumonia. Lungs were exuding consolidation foci, bilateral pectoral effusions, consolidation on the 8th day of hospitalization (A). After treatment, on the 18th day of hospitalization (B), the image of four patients improved, and the patients’ lung exudation, consolidation, and bilateral pleural effusion were less than before.

Figure 2

Chest X-ray of a 36-year-old pregnant female patient with C. psittaci pneumonia and died of septic shock, DIC, and multiple organ failure. (A) on the first day of hospitalization, (B) on the third day of hospitalization, (C) on the sixth day of hospitalization, (D) on the seventh day of hospitalization.

Treatment and Outcome

After C. psittaci pneumonia was confirmed, one of the three male patients received symptomatic anti-infective treatment with moxifloxacin (400mg ivgtt qd) and with adjuvant non-invasive ventilator therapy. The other two male patients received symptomatic anti-infective therapy with doxycycline (100mg po q12h) and were treated with high flow nasal cannula therapy. All three male patients were improved and discharged after more than 10 days of treatment. Two female patients were transferred to our hospital after invasive ventilator adjuvant treatment with tracheal intubation from other hospitals. They were treated with moxifloxacin (400mg ivgtt qd) combined with doxycycline (100mg po q12h) for symptomatic anti-infective therapy. The two female patients were treated with ECOM and ventilator adjuvant therapy due to their critical illness. One patient was removed ECOM after five days of treatment with blood oxygen saturation and oxygen partial pressure was significantly improved. After 3 days of consecutive treatment, the invasive ventilator was removed. The patient continued to receive treatment and was discharged from the hospital. Another 35-years old female patient (case 5) was critically ill and the disease progressed rapidly and finally die. One week before admission, the B-mode ultrasound showed that the patient was in early intrauterine pregnancy with a size of about 6 weeks, with no germ and heartbeat. The patient was transferred to the respiratory ICU of our hospital after oral endotracheal intubation with a high fever of 39 °C and blood oxygen saturation of 65%. Chest radiograph showed multiple exudative lesions in both lungs and pleural effusion on the right side. After admission, she was given meropenem combined with moxifloxacin and ganciclovir. On the third day after admission, mNGS of blood indicated C. psittaci, with a sequence number of 533. mNGS of BAFL indicated Klebsiella pneumoniae, with a sequence number of 175. Then the patient was given an injection of doxycycline. On the 7th day of admission, the patient’s blood oxygen saturation was still 70% (through endotracheal intubation and invasive ventilator), and ECMO treatment was performed. On the 8th day of admission, the patient died due to septic shock, disseminated intravascular coagulation (DIC), and multiple organ failures.

Literature Review

We searched the databases including PubMed, EMBASE, Web of Science, Wanfang, and Chinese National Knowledge Infrastructure (CNKI) from 1st Jan. 2010 to 1st Oct. 2020. The searching strategy was “Chlamydia psittaci” or “Chlamydia psittaci pneumonia”. Dr. Jian Qu and Dr. Wen-Cheng Gong reviewed all relevant articles to identify potentially eligible studies. We conducted this literature review of patients with C. psittaci pneumonia to summarize the diagnostic methods and anti-infective drugs, and we also summarized the clinical outcome and history of exposure to avian or poultry of these infection patients to provide a reference for future C. psittaci pneumonia infection patients’ diagnosis and treatment. The data about authors, reported time, number of cases, ethnics, history of exposure to avian or poultry, anti-infective drugs regiment, and clinical outcome were collected. We found 794 publications. After excluded the full text could not be found or provided no information we needed about C. psittaci pneumonia or no original data or the Chlamydia was not specified. Finally, 19 articles were enrolled in further review.

The summary of the detailed information was shown in Table 3. The articles were published from 2012 to 2020. With the development of detection technology, the number of articles reported tends to increase (Figure 3). Since 2019, there were 13 articles reported C. psittaci pneumonia and among them, 10 articles using mNGS to detect C. psittaci. There was a total of 69 C. psittaci pneumonia patients were reported and most patients had a history of exposure to avian or poultry. Most patients treated with doxycycline, moxifloxacin, meropenem, or their combinations, and three patients used ECOM support. Most of the patients’ treatment improved and four patients died.

Table 3

Summary of Case Series and Case Report of C. psittaci Pneumonia

Author	Reported Time	Number of Reported Cases	Reported Area	Methods	Anti-Infective Drugs	Clinical Outcome	History of Exposure to Avian or Poultry
Gacouin4	2012	13	France	mNGS	Tetracycline ± Erythromycin/Levofloxacin	11 patients improved, 2 patients died	Yes
Laroucau33	2013	8	France	PCR	Macrolides + Cephalosporins	Improved	Yes
Chau34	2015	3	Hong Kong	PCR	Doxycycline	Improved	Yes
Mair-Jenkins35	2015	4	United Kingdom	Serology and PCR	Not stated	Improved	No history of direct contact, but someone raises pigeons near the office building
Spoorenberg36	2016	7	Netherlands	PCR, MIF	2 patients used Tetracycline, Macrolide or Quinolone; 1 patients used β-lactam	Improved	6 patients had an exposure history
Cipriano37	2016	1	Portugal	IIF	Amoxicillin/Clenauic Acid, Azithromycin	Improved	Yes
Qiu29	2019	1	China	mNGS	Doxycycline + Moxifloxacin	Improved	No
Zhu38	2019	1	China	mNGS	Moxifloxacin	Improved	Yes
Shi39	2019	1	China	PM-seq	Piperacillin/tazobactam + Minocycline	Improved	Yes
Liu28	2019	1	China	Antibody test	Moxifloxacin	Improved	Yes
Gu13	2019	5	China	mNGS, IIF	1 patient used Minocycline +Erythromycin	Improved	4 patients had an exposure history
					2 patients used Doxycycline+ Moxifloxacin
					2 patients used Moxifloxacin
Chen14	2020	9	China	mNGS	Minocycline	8 patients improved, 1 patient died	7 patients had an exposure history
He40	2020	1	China	mNGS	Doxycycline	Improved	Yes
Chen30	2020	1	China	mNGS	Moxifloxacin +Cefoperazone Sodium Sulbactam/Meropenem	Improved	Not stated
Katsura41	2020	1	Japan	PCR	Meropenem+Gamma globulin	Died	Yes
Zhang42	2020	1	China	mNGS	Doxycycline +Moxifloxacin	Improved	Yes
Zhang43	2020	1	China	mNGS	Doxycycline + ceftazidime/meropenem	Improved	No
Fernández44	2020	5	Murcia	mNGS	3 patients used Levofloxacin	Improved	Yes
					+ Doxycycline
					2 patients used Cephalosporin
					+ Doxycycline
Luo45	2020	5	China	mNGS	2 patients used Doxycycline	Improved	Yes
					1 patients used Doxycycline + Meropenem
					1 patients used Doxycycline + Piperacillin/tazobactam
					1 patients used Doxycycline + Levofloxacin

Abbreviations: mNGS, metagenomics next-generation sequencing; PCR, polymerase chain reaction; MIF, micro-Immunofluorescence; IIF, indirect immunofluorescence; PM-seq, pathogenic microorganisms sequencing.

Figure 3

The summary of C. psittaci pneumonia literature review from 2010 to 2020 year.

Discussion

We retrospectively analyzed five cases of psittacosis pneumonia diagnosed using mNGS and summarized the clinical characteristics including disease progression, treatments, and outcomes, etc. Moreover, we also carried out a literature review that summarized the existing research and reports about C. psittaci pneumonia. In our study, five C. psittaci pneumonia patients confirmed by mNGS aged from 36 to 66 years with three males. 60% of patients had underlying diseases Type 2 diabetes. Three of these five patients had a history of contact with avian or poultry. All patients had a high fever over 38.5 °C, cough, hypodynamia, hypoxemia, and dyspnea on admission. Two patients had invasive ventilator support and ECOM support. The levels of CRP, PCT, and ESR on admission and follow-up were all higher than normal values. Doxycycline or moxifloxacin monotherapy was accounted for 1/5 (20%) and 2/5 (40%) patients, and combination therapy was accounted for 2/5 (40%) patients. Four patients improved and were discharged, and one patient died due to multiple organ failures and disseminated intravascular coagulation.

The lung manifestations are mainly cough, dry cough, shortness of breath, the rapid progress of lung disease, and occasionally acute respiratory distress syndrome (ARDS).15 In the five cases reported in this article, 3 patients had ARDS. And there were even case reports showing only abnormal liver function.16 In the initial auscultation, the lung lesions of C. psittaci pneumonia were often underestimated. The chest radiograph showed infiltrating patches with uneven density, which can seriously affect all lung lobes. CT of the lungs showed consolidation or ground glass-like changes, especially the lower lung, with pleural involvement and pleural effusion.17 After treatment, the patients’ cough and fever improved, but the oxygenation index recovered slowly. According to the reporting of literature, the absorption of the lesion was slow, with an average absorption time of 6 weeks, up to 20 weeks.18 The laboratory inspection parameters of the five patients on admission showed that two patients had WBC > 10×109/L, and three cases had PCT > 10 ng/mL, and the chest radiology presented mainly consolidation. The mNGS detection of case 5 also showed Klebsiella pneumoniae infection in addition to C. psittaci infection, but there were no other bacteria was isolated in the other four cases. Although other bacteria were not found in culture, other bacterial coinfections cannot be ruled out because of the high WBC and PCT value, and the chest radiology characterized by consolidation. Especially, some patients were carried out invasive ventilator support or ECOM. Therefore, patients are at risk for hospital-acquired pneumonia and ventilator-associated pneumonia infection. Before admission, the community-acquired pneumonia was also treated with antibiotics such as cephalosporins and quinolones in other hospitals before the diagnosis of C. psittaci pneumonia.

According to the control requirements of C. psittaci issued by the National Public Health Veterinary Association, the confirmed diagnosis of human C. psittaci pneumonia only needs to meet one of the following two standards: 1). Isolate C. psittaci from respiratory specimens (sputum, pleural fluid, tissue, etc.) or blood specimens 2). Serological examination: Measure the C. psittaci IgG antibody in the acute and convalescent phases during the interval of 2–4 weeks, the convalescent phase is more than four times higher than the acute phase. If the patient meets one of the following two standards, it may be infected: 1). Serum examination, C. psittaci IgM ≥ 32; 2). C. psittaci DNA can be detected through PCR amplification of respiratory specimens (sputum, pleural fluid, tissue, etc.).19 Since C. psittaci is strictly intracellular parasitic, its direct isolation and cultivation are very difficult and cannot be carried out routinely. At present, the clinical presentation and the positive serological result using MIF with paired sera are the most often used diagnostic methods of C. psittaci.1 MIF20 and PCR gene expansion detection have become auxiliary detection of molecular biological diagnosis due to their high sensitivity and specificity.21 Although MIF is more sensitive and specific than complement fixation (CF) tests, the test still displays cross-reactivity with other Chlamydia species in some instances.22 PCR testing is not clinical laboratory improvement amendments validated currently. mNGS can be used to detect pathogens that cannot be detected by traditional methods.23 Patients introduced in this article were all severely infected when they were admitted to our hospital, with respiratory failure, and dry cough without sputum. Due to the high risk of bronchoscopy and difficulty in taking respiratory tract specimens, the blood or BAFL samples of patients were sent out for mNGS testing and finally reported C. psittaci infection. Early pathogenic diagnosis can greatly benefit patients, and of the 5 patients reported in this article, 4 patients improved and were discharged. Our literature review also found that with the development of technology, the number of C. psittaci detected increased year by year, and the articles reported C. psittaci pneumonia via mNGS increased year by year (Figure 3 and Table 3).

Tetracyclines, macrolides, and quinolones can interfere with DNA and protein synthesis, therefore, these three kinds of antibiotics can be used to treat C. psittaci.24 At present, both in China and other country, tetracyclines are the first choice for the treatment of C. psittaci pneumonia including tetracycline, doxycycline, and minocycline.25,26 In severe cases, doxycycline can be administered intravenously. The treatment of the patient in case 2 with doxycycline is also effective. Macrolide drugs such as azithromycin and fluoroquinolones have been confirmed to have antibacterial activity against C. psittaci in vitro,26,27 In particular, moxifloxacin has strong antibacterial activity against Chlamydia, and there are case reports at home and abroad that the use of fluoroquinolone drugs is effective.28–31 Given the lack of experience in the use of tetracyclines in our hospital, Chlamydia trachomatis, which is the same species as C. psittaci in my country, is highly resistant to tetracycline,32 so the treatment for case 1 patient used moxifloxacin and it was effective. After five patients were treated, four patients were improved and discharged. Among them, one patient was treated with moxifloxacin, two patients were treated with doxycycline, and the other two patients were treated with moxifloxacin plus doxycycline. Current C. psittaci Pneumonia treatment guidelines recommend the addition of macrolide or quinolone to the initial regimen of severe C. psittaci in any case. According to current reports, it is unclear whether combination medication is more effective than single medication for patients. Further case-control studies with larger samples are needed to find the optimal treatment.

In this article, we have searched the relevant literature. At present, most cases of human infection with C. psittaci are reported in scattered cases and details are listed in Table 3. With the development of detection technology, mNGS became a routine examination for etiology. Therefore, more and more C. psittaci pneumonia was diagnosed and treated according to guidelines. Our literature review summarized 19 articles including 69 C. psittaci pneumonia patients, Patients in 11 articles were identified by mNGS, including 9 articles were reported in China. In recent years, the reports of mNGS for C. psittaci pneumonia diagnosis have increased, especially in China. We found that most patients had a history of exposure to avian or poultry. Therefore, epidemiological data combined with mNGS detection is helpful for the early diagnosis of C. psittaci pneumonia. Most patients are treated with doxycycline, moxifloxacin, or their combinations. Three patients used ECOM support and they are all improved. Among these 69 patients in our literature review, 65 patients of C. psittaci pneumonia improved and four patients died. In the future, further case-control studies with a large sample size are needed to find better diagnostic methods and better anti-infective drugs.

Conclusions

The history of contact with avian or poultry and the typical symptoms (high fever over 38.5 °C, cough, hypodynamia, hypoxemia, and dyspnea) are important for C. psittaci pneumonia diagnosis. Moxifloxacin, doxycycline, or their combinations are effective treatment options for C. psittaci pneumonia. mNGS is a promising rapid diagnosis tool, which may increase the detection rate and shorten the diagnosis time of C. psittaci pneumonia. Further case-control studies are needed to confirm.