Olena Vergun1, Dzhamal Rakhmetov1, Oksana Shymansk1, Oleksandr Bondarchuk1, Eva Ivanišová2, Miroslava Kačaniová3, Olga Grygorieva4, Ján Brindza5. 1. Department of Cultural Flora, M. M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine, Kyiv, Ukraine. 2. Department of Technology and Quality of Plant Products, Slovak University of Agriculture in Nitra, Slovakia. 3. Department of Fruit Growing, Viticulture and Enology, Slovak University of Agriculture in Nitra, Slovakia. 4. Department of Fruit Plant Acclimatization, M. M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine, Kyiv, Ukraine. 5. Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovakia.
Abstract
BACKGROUND: The search for new plant raw material as a potential source of antioxidants is still ongoing. This study aimed to evaluate the antioxidant and antimicrobial capacity of the plant raw material of Crambe spp. during vegetation. METHODS: The free radical scavenging activity and molybdenum reducing power of the extracts were used to determine antioxidant activity. The quantification of polyphenol compounds was conducted with Folin-Ciocalteu reagent. Flavonoids and phenolic acids were also determined. The disc diffusion method was used to determine antimicrobial activity. RESULTS: It was determined that the free radical scavenging activity, assessed using the DPPH-method, was 4.38-8.20 mg TE/g DW, the molybdenum reducing power of the extracts was 40.07-129.12 mg TE/g DW, total polyphenol content was 20.24-70.88 mg GAE/g DW, total flavonoid content was 5.73-29.92 mg QE/g DW, and phenolic acid content was 3.00-10.63 mg CAE/g DW. Antimicrobial activity depended on the stage of growth and the part of the plant used. CONCLUSIONS: Crambe spp. possess the antioxidant and antimicrobial potential to mean that they could be used in pharmaceutical studies and the food industry.
BACKGROUND: The search for new plant raw material as a potential source of antioxidants is still ongoing. This study aimed to evaluate the antioxidant and antimicrobial capacity of the plant raw material of Crambe spp. during vegetation. METHODS: The free radical scavenging activity and molybdenum reducing power of the extracts were used to determine antioxidant activity. The quantification of polyphenol compounds was conducted with Folin-Ciocalteu reagent. Flavonoids and phenolic acids were also determined. The disc diffusion method was used to determine antimicrobial activity. RESULTS: It was determined that the free radical scavenging activity, assessed using the DPPH-method, was 4.38-8.20 mg TE/g DW, the molybdenum reducing power of the extracts was 40.07-129.12 mg TE/g DW, total polyphenol content was 20.24-70.88 mg GAE/g DW, total flavonoid content was 5.73-29.92 mg QE/g DW, and phenolic acid content was 3.00-10.63 mg CAE/g DW. Antimicrobial activity depended on the stage of growth and the part of the plant used. CONCLUSIONS: Crambe spp. possess the antioxidant and antimicrobial potential to mean that they could be used in pharmaceutical studies and the food industry.