Literature DB >> 33876274

Standard operating procedure for NanoTemper Monolith measurements.

Arthur Sedivy1.   

Abstract

This manuscript is intended to give a step-by-step standard operating procedure (SOP) about how to properly conduct a microscale thermophoresis (MST), also known as temperature-related intensity change (TRIC) experiment using the NanoTemper Technology GmbH Monolith instruments.

Keywords:  Interaction; K D; MST; TRIC; Thermophoresis

Year:  2021        PMID： 33876274     DOI： 10.1007/s00249-021-01534-4

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  3 in total

Review 1.  Click chemistry, a powerful tool for pharmaceutical sciences.

Authors:  Christopher D Hein; Xin-Ming Liu; Dong Wang
Journal:  Pharm Res       Date:  2008-05-29       Impact factor: 4.200

2.  Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions.

Authors:  Carla P Guimaraes; Martin D Witte; Christopher S Theile; Gunes Bozkurt; Lenka Kundrat; Annet E M Blom; Hidde L Ploegh
Journal:  Nat Protoc       Date:  2013-08-29       Impact factor: 13.491

3.  Site-specific N-terminal labeling of proteins using sortase-mediated reactions.

Authors:  Christopher S Theile; Martin D Witte; Annet E M Blom; Lenka Kundrat; Hidde L Ploegh; Carla P Guimaraes
Journal:  Nat Protoc       Date:  2013-08-29       Impact factor: 13.491
  3 in total
  2 in total

1.  The Acid-Base/Deprotonation Equilibrium Can Be Studied with a MicroScale Thermophoresis (MST).

Authors:  Paweł Mateusz Nowak; Michał Woźniakiewicz
Journal:  Molecules       Date:  2022-01-21       Impact factor: 4.411

2.  The First Online Capillary Electrophoresis-Microscale Thermophoresis (CE-MST) Method for the Analysis of Dynamic Equilibria-The Determination of the Acidity Constant of Fluorescein Isothiocyanate.

Authors:  Paweł Mateusz Nowak; Maria Klag; Gabriela Kózka; Małgorzata Gołąb; Michał Woźniakiewicz
Journal:  Molecules       Date:  2022-08-06       Impact factor: 4.927
  2 in total

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