| Literature DB >> 3386880 |
A Martinez1, J Vitórica, J Satrústegui.
Abstract
Calcium homeostasis in synaptosomes is altered during ageing. The cytosolic free calcium concentration, [Ca2+]i was determined in synaptosomes and crude synaptosomal fractions from 3- and 24-month-old rats with the fluorescent indicator quin-2. The [Ca2+]i were around two times higher in 24-month-old rats than in adults, both under resting conditions and after K depolarization. This difference was still observed after incubation with an endogenous heavy metal chelator. To avoid the calcium buffering effect of quin-2, [Ca2+]i values were determined with the use of a null-point method and with fura-2. These methods confirmed the increase in [Ca2+]i with age in synaptosomes. The increase in [Ca2+]i in nerve endings may be pathologically important in brain ageing.Entities:
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Year: 1988 PMID： 3386880 DOI： 10.1016/0304-3940(88)90234-0
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046