Effects of a combined therapy of bortezomib and ionizing radiation on chondrosarcoma three-dimensional spheroid cultures.

Chondrosarcomas represent a heterogeneous group of primary bone cancers that are characterized by hyaline cartilaginous neoplastic tissue and are predominantly resistant to radiation and chemotherapy. However, adjuvant radiotherapy is often recommended in inoperable cases or after incomplete resections. To improve the efficiency of treatment, the present study tested a combination therapy with ionizing radiation (IR) and the proteasome inhibitor bortezomib. Using a three-dimensional (3D) spheroid model, 0-20 Gy of IR was applied to chondrosarcoma cells and healthy human chondrocytes. Following combined treatment with IR and bortezomib, the cell cycle distribution, apoptotic induction, the survivin pathway, autophagy and DNA damage were evaluated. Both cell types exhibited a slight decrease in viability following increasing doses of IR; the chondrosarcoma cells demonstrated a significant dose-dependent increase in the expression levels of the DNA damage marker histone H2AX phosphorylation at serine 139 (γH2AX). The combination treatment with bortezomib significantly decreased the cell viability after 48 h compared with that in irradiated cells. High-dose IR induced a G2/M phase arrest, which was accompanied by a decrease in the number of cells at the G1 and S phase. Co-treatment with bortezomib changed the distribution of the cell cycle phases. The mRNA expression levels of the proapoptotic genes Bcl-2-associated X protein (Bax) and Bak were significantly increased by bortezomib treatment and combination therapy with IR. In addition, the combination therapy resulted in a synergistic decrease of the expression levels of survivin and its corresponding downstream pathway molecules, including heat shock protein 90, X-linked inhibitor of apoptosis protein, smad 2 and smad 3. Comparative analyses of γH2AX at 1 and 24 h post-IR revealed efficient DNA repair in human chondrosarcoma cells. Therefore, additional bortezomib treatment may only temporarily improve the radiation sensitivity of chondrosarcoma cells. However, the inhibition of the survivin pathway by the combined treatment with IR and bortezomib, observed in the present study, revealed a novel aspect in the tumor biology of chondrosarcoma 3D spheroid cultures and may represent a potential target for therapy. Copyright: © Lohberger et al.

Introduction

Chondrosarcomas are heterogeneous, mostly slow-growing, primary malignancies of the bone that are characterized by hyaline cartilaginous neoplastic tissue formation (1,2). Surgery remains the gold standard, although macroscopically complete resection is often impeded by the presence of adjacent critical structures. Therefore, adjuvant or definitive radiotherapy is frequently recommended for inoperable cases or following incomplete resection (3–8).

Particle radiotherapy with protons and carbon ions has emerged as a novel therapy option and has been demonstrated to be beneficial in the treatment of chondrosarcomas. The physical and biological characteristics of particles provide the possibility to deliver higher doses to larger target volumes while adhering to the tolerance doses of normal tissue structures surrounding the tumor (9–11). However, these complex technologies are only available at a limited number of particle radiation centers worldwide; thus, patient access is highly restricted. Therefore, it is crucial to optimize widely available photon therapy treatment despite lower therapeutic success. Specific focus should be drawn to combinatorial approaches with chemotherapeutics.

Previous studies have demonstrated a synergistic effect of the proteasome inhibitor bortezomib and ionizing radiation (IR) on the viability of various types of cancer, including oral (12), cervical (13) and colorectal (14) cancer, as well as esophageal squamous cell carcinoma (15), and suggested a radiosensitizing effect of bortezomib. The proteasome pathway serves an essential role in the regulation of a variety of cellular processes associated with the proliferation and survival of tumor cells (12–15). Our previous study demonstrated that bortezomib exhibits antitumor activity against chondrosarcoma cells (16).

Cell-based assays are the key tool to assess the potential efficacy of new treatment options in cancer research. Cellular heterogeneity, morphology and the interactions of cells grown in three-dimensional (3D) cultures are similar to the in vivo microenvironment (17). 3D spheroids are formed from cells in a variety of stages, including proliferating, quiescent, hypoxic, apoptotic and necrotic cells (18). The outer spheroid layer, which is exposed the most to the medium, is mostly comprised of proliferating cells (19). Thus, the cellular processes of these cells mimic those occurring in vivo (20). Therefore, the present study used the 3D spheroid model for the experiments.

This aim of the present study was to determine the potential radiosensitizing effects of concomitant bortezomib treatment in human chondrosarcoma 3D spheroid cultures and to elucidate the underlying cellular processes.

Materials and methods

3D spheroid cultures

Chondrosarcoma SW1353 cells (ATCC® HTB-94™; LGC Standards, Ltd.) were cultured in Dulbecco's modified Eagle's medium (DMEM-F12) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 1% penicillin/streptomycin and 0.25 µg amphotericin B (all Gibco; Thermo Fisher Scientific, Inc.). Human healthy chondrocytes (HCs) (cat. no. 402-05a; Cell Applications, Inc.) were cultured in DMEM-F12 supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, 0.25 µg amphotericin B, TGFβ3 and FGFβ (both 1 ng/ml; Sigma-Aldrich; Merck KGaA). The SW1353 cell line was verified by short tandem repeat analysis utilizing a PowerPlex™ 16 System kit (Promega Corporation). The primary chondrocytes were used between passages 2 and 4. Regular testing for mycoplasma infection was conducted by PCR. Cells were incubated at 37°C in a humidified atmosphere with 5% CO2 and passaged by trypsinization upon reaching confluence. 3D spheroids were generated by the hanging drop method. A total of 5,000 cells/spheroid mixed with 1.2% Methocel A4M (Sigma-Aldrich; Merck KGaA) dissolved in the culture medium were placed on the cover of a petri dish and incubated upside-down for 48 h at 37°C in a humidified atmosphere with 5% CO2. Subsequently, the spheroids were transferred to appropriate culture plates. To avoid adhesion of the spheroid cultures, 1% poly(2-hydroxyethyl methacrylate)-coated plates were used.

Hematoxylin and eosin stain

For histological and immunohistochemical evaluation, the chondrosarcoma 3D spheroids were fixed for 30 min with 10% formalin (Carl Roth GmbH) at room temperature and embedded in paraffin. For morphological evaluation, staining with hematoxylin and eosin was performed. Briefly, following a 15-min incubation in xylene (Merck KGaA) and a descending ethanol series (100, 90, 70 and 50%; 3 min each), the sections were stained with hematoxylin and eosin for 2 min each at room temperature. The embedding of the samples was performed with n-buthyl-acetate and Entellan® (both Merck KGaA).

X-ray irradiation

X-ray irradiation was performed at the Division of Biomedical Research, Medical University Graz (Graz, Austria) with an RS-2000 biological irradiator (RadSource Technologies, Inc.) equipped with a 3-mm Al/0.3-mm Cu filter and a current of 25 mA to provide 160 kV X-rays at a dose rate of 2.108 Gy/min.

Cell viability assay

For the dose-response relationship, 100 spheroids each of HC and SW1353 were pretreated with the IC50 concentration of 5 nM bortezomib (Selleck Chemicals) for 24 h. Cell viability was determined with the CellTiter-Glo® 3D Cell Viability assay (Promega Corporation) at 3 and 48 h post-administration of 0 (control) to 20 Gy IR. Background reference values were derived from the culture media. Absorbance was measured with a LUMIstar™ microplate luminometer (BMG Labtech GmbH), and the mean ± SD was calculated (HC, n=3; SW1353, n=7; performed in biological quadruplicates).

Cell cycle analysis

Following pretreatment with 5 nM bortezomib and treatment with 0, 5 or 20 Gy IR, spheroid cultures were harvested at 3 and 48 h. A single-cell suspension was prepared by trypsinization, and cells were fixed with 70% ice-cold ethanol for 10 min at 4°C. Prior to flow cytometry, the cell pellet was resuspended in PI-staining buffer (50 µl/ml PI, 100 µl/ml RNAse A, 0.1% Triton X-100 and 0.1% sodium citrate) and incubated for 20 min at room temperature. Cell cycle distribution was measured with a CytoFlexLX flow cytometer (Beckman Coulter, Inc.) and analyzed using ModFit LT software Version 4.1.7 (Verity software house).

Annexin V/PI apoptosis assay

To detect translocated phosphatidylserine, the eBioscience™ FITC Annexin V Apoptosis Detection kit (Thermo Fisher Scientific, Inc.) was used 48 h after treatment with IR and/or bortezomib according to the manufacturer's instructions. Flow cytometry analysis was performed with a CytoFlexLX flow cytometer, and a total of 10,000 events were collected. Cells were identified in the side and forward scatter with a linear scale. Fluorescence signals were presented with a logarithmic scale. Compensation was performed by single Annexin and PI measurements, and analyzed by FCS3 express software Version 7 (De Novo Software, LLC). The graphical representations of Annexin−/PI− (viable cells), Annexin+/PI− (early apoptotic cells), Annexin+/PI+ (late apoptotic cells) and Annexin−/PI+ (necrotic cells) were plotted as stacked bars (n=3).

Reverse transcription-quantitative (RT-q)PCR

Total RNA was isolated following bortezomib pretreatment and 48 h post-5 Gy IR from spheroid cultures using the RNeasy Mini kit (Qiagen GmbH) and DNase-I treatment according to the manufacturer's instructions. A total of 1 µg RNA was reverse-transcribed with an iScript cDNA Synthesis kit, (Bio-Rad Laboratories, Inc.) using a blend of oligo(dT) and random hexamer primers. Amplification was performed with the SsoAdvanced Universal SYBR®-Green Supermix (Bio-Rad Laboratories, Inc.) and measured by the CFX96 Touch PCR instrument (BioRad Laboratories, Inc.). Each qPCR run comprised a standard three-step PCR temperature protocol (95°C for 30 sec, followed by 40 cycles of 95°C for 10 sec and 60°C for 20 sec), followed by a melting curve protocol to confirm a single gene-specific peak and to detect primer dimerization (65–95°C). Relative quantification of expression levels was performed using the ΔΔCq method based on the geometric mean of the internal controls TATA-box binding protein and ribosomal protein lateral stalk subunit P0. The expression levels (Cq) of the target genes were normalized to the reference genes ribosomal protein (RPL; cat. no. QT00075012), TATA box-binding protein (TBP; cat. no. QT00000721), and beta-2-microglobulin (B2M; cat. no. QT00088935) (all Qiagen GmbH) (ΔCq), and the difference between the ΔCq value of the test and control samples produced the ΔΔCq value. Finally, the expression ratio was expressed as 2−ΔΔCq (21). The following QuantiTect primer assays (Qiagen GmbH) were used for qPCR: Matrix metalloproteinases (MMP)2 (cat. no. QT00088396), MMP9 (cat. no. QT00040040), Bax (cat. no. QT00997381), Bcl-2 antagonist/killer-1 (Bak) (cat. no. QT00228508), Noxa (cat. no. QT00074438), and survivin (BIRC5) (cat. no. QT00081186). The following primers were used: Bcl-2 forward, 5′-GGAGGATTGTGGCCTTCTTTG-3′ and reverse, 5′-GCCGGTTCAGGTACTCAGTCAT-3′; and TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2) forward, 5′-ACCAACGAGCTGAAGCAGATG-3′ and reverse, 5′-CAAGCAATGCCACTTTTGGA-3′. Untreated cells served as the reference.

Western blot analysis

Whole cell protein extracts were prepared 48 h post-bortezomib pretreatment and 5 Gy IR from spheroid cultures using a lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM NaF, 1 mM ethylenediaminetetraacetic acid, 1% nonylphenol-40 and 1 mM Na3VO4], a protease and phosphatase inhibitor cocktail (PPC1010; Sigma-Aldrich; Merck KGaA) and subsequent sonication. Protein concentration was determined with the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Inc.). A total of 10–20 µg of proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and blotted on Amersham™ Protran™ Premium 0.45 µM nitrocellulose membranes (Cytiva). For microtubule-associated protein 1A/1B-light chain 3 (LC3)BI–II immunoblotting, the cells were treated with the lysosomal protease inhibitors E64d and pepstatin A (10 µg/ml; both Sigma-Aldrich; Merck KGaA), which were added to the cell culture medium to inhibit the autophagic flux and the degradation of LC3B-II, and improve the representation of the LC3B-II levels. All other blots were incubated with primary antibodies against the apoptotic markers Bax, Bcl-2, Noxa, TRAIL-R2, cleaved poly(ADP-ribose) polymerase (PARP), survivin and its downstream components heat shock protein 90 (HSP90), X-linked inhibitor of apoptosis protein (XIAP), smad 2 and smad 3, the DNA damage marker phospho-histone γH2AX and β-actin as the loading control. All antibody information, dilutions and incubation times are listed in Table I. Subsequently, the blots were incubated with a horseradish peroxidase-conjugated secondary antibody (Dako; Agilent Technologies, Inc.) for 1 h at room temperature, followed by overlay with Amersham™ ECL™ prime western blotting detection reagent (Cytiva). The resulting chemiluminescence signals were detected with the ChemiDocTouch Imaging System (Bio-Rad Laboratories, Inc.), and the images were processed with ImageLab 5.2 software (Bio-Rad Laboratories, Inc.).

Table I.

Antibodies used for the western blot analysis.

	Primary antibody				Secondary antibody
Target	Supplier and cat. no.	Dil.	Inc.	Target	Supplier and cat. no.	Dil.	Inc.
Bax	CS #5023	1:1,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
Bcl-2	CS #3498	1:1,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
Noxa	Thermo 1-41000	1:1,000	ON 4°C	Anti-mouse	Dako P0447	1:3,000	1 h 4°C
TRAIL-R2	CS #3696	1:1,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
cPARP	Thermo 436400	1:2000	ON 4°C	Anti-mouse	Dako P0447	1:3,000	1 h 4°C
LC3BI–II	CS #2775	1:1,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
Survivin	CS #2802	1:1,000	ON 4°C	Anti-mouse	Dako P0447	1:3,000	1 h 4°C
HSP90	CS #4877	1:3,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
XIAP	CS #2045	1:3,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
Smad2	sc-101153	1:1,000	ON 4°C	Anti-mouse	Dako P0447	1:3,000	1 h 4°C
Smad3	sc-101154	1:1,000	ON 4°C	Anti-mouse	Dako P0447	1:3,000	1 h 4°C
γH2AX	CS #2577	1:1,000	ON 4°C	Anti-rabbit	Dako P0448	1:3,000	1 h 4°C
β-actin	sc-47778	1:10,000	ON 4°C	Anti-mouse	Dako P0447	1:15,000	1 h 4°C

TRAIL-R2, TNF-related apoptosis-inducing ligand receptor 2; cPARP, cleaved poly(ADP-ribose) polymerase (PARP); HSP90, heat shock protein 90; XIAP, X-linked inhibitor of apoptosis protein; γH2AX; histone H2AX phosphorylation at serine 139; Dil., dilution; Inc, incubation; CS, Cell Signaling Technology, Inc.; Thermo, Thermo Fisher Scientific, Inc.; sc, Santa Cruz Biotechnology, Inc., ON, overnight (ON).

Gene expression profiling

Gene expression profiling was performed using the Thermo Fisher Ion AmpliSeq RNA workflow. cDNA of 50 ng RNA from chondrosarcoma spheroid cultures was synthesized using the SuperScript IV™ Vilo™ Master Mix (cat. no. 11756500; Thermo Fisher Scientific, Inc.; 25°C for 10 min, 50°C for 10 min, 85°C for 5 min and hold at 10°C). AmpliSeq PCR and library preparation was performed using the Ion AmpliSeq Library Kit Plus and an Ion AmpliSeq RNA Panel (thermocycling conditions: 99°C for 2 min, followed by 27 cycles of 99°C for 15 sec and 60°C for 4 min, and hold at 10°C). Sequencing was performed on an Ion S5XL benchtop sequencer using the 540 Chip kit and the 200 base pair workflow (single-end; all Thermo Fisher Scientific, Inc.) to a total depth of ~1 million reads per sample. The amplicon sequencing depth was determined using the AmpliSeq RNA Ion Torrent Suite Plugin (version 4.4.0.4), and individual gene expression was calculated as amplicon reads per million total reads (RPM). The heatmap of the RNA sequencing data is presented as fold-change regarding the expression without IR (control) and 1 or 24 h post-IR of key player genes in terms of base excision repair (BER), mismatch mediated repair (MMR), nucleotide excision repair (NER), homology directed repair (HDR) and the nonhomologous end-joining (NHEJ). The sequencing coverage and the quality statistics of the AmpliSeq RNA sequencing are presented in Table SI. The corresponding raw data are presented in Table SII.

Immunocytochemistry

For immunocytochemical staining, chondrosarcoma cells were pretreated with the IC50 concentration of 5 nM bortezomib for 24 h and irradiated with 0 (control) and 5 Gy. At 1 and 24 h post-IR, the cells were fixed with 4% paraformaldehyde for 30 min. The slides were incubated with a γH2AX antibody (Merck KGaA; cat. no. 05-636; 1:200) for 1 h, the bridge antibody (Dako; cat. no. P0260; 1:200) for 30 min, the polymer (rabbit-ON-rodent-horseradish peroxidase; Biocare Medical, LLC; RMR622H; ready to use) for 30 min and aminoethyl carbazole substrate chromogen (Dako; Agilent Technologies, Inc.) for 3 min (all room temperature). The reaction was stopped with phosphate-buffered saline, and hematoxylin core staining was performed. Images with ×100 magnification were captured under an Olympus BX51 transmitted-light microscope (Olympus Corporation) and five areas were analyzed per slide.

Statistical analysis

Data are presented as the mean ± SD or SEM as indicated. SigmaPlot 14.0 (Systat Software, Inc.) was used to generate the figures and graphs. Comparisons between untreated control, bortezomib- and IR-treated cells were performed by one way ANOVA followed by post hoc analysis using the Holm-Sidak test. Two-sided P<0.05 was considered to indicate a statistically significant difference.

Results

Increasing IR intensities affect spheroid cultures

The preparation of 3D spheroid cultures using the hanging drop method is schematically presented in Fig. 1A. Chondrosarcoma cells (SW1353) and HCs formed clearly defined spheroids after 24–48 h.

Figure 1.

Chondrosarcoma 3D spheroid cultures. (A) The preparation of hanging drop 3D spheroid cultures and a representative image of H&E staining (×400 magnification). (B) Effects of increasing IR doses on cell viability. (C) Effects of pretreatment with Bor and treatment with 5 and 20 Gy IR on the viability of SW1353 chondrosarcoma cells and HC. Data were normalized to untreated controls (0 Gy). Red lines represent mean values. HC, n=3; SW1353, n=7. IR, ionizing radiation; Ctrl, control; Bor, bortezomib; HC, healthy chondrocytes; 3D, three-dimensional.

Increasing IR doses only marginally reduced the viability of SW1353 3D spheroids at 3 h; the reduction in HC viability appeared to be more pronounced compared with that observed in chondrosarcoma cells (Fig. 1B). Additionally, this downward trend of viability appeared also to be more pronounced in HC at 48 h compared with that in the chondrosarcoma cells. Treatment with the IC50 concentration of bortezomib significantly reduced viability in the two cell types (Fig. 1C). Treatment with the respective IC50 concentrations (16) reduced the cell viability to 65.04±11.1% for SW1353 and to 60.37±6.10% for HC. Additional 5 or 20 Gy IR caused a further significant reduction to 58.61±12.24% for SW1353 and 33.77±4.56% for HC, respectively. Therefore, HC exhibited higher sensitivity for both treatments compared with that of the chondrosarcoma cells.

Effects of IR on cell cycle distribution

Flow cytometry analysis was performed to determine the cell cycle profiles of chondrosarcoma spheroid cultures exposed to 5 and 20 Gy IR with or without their respective IC50 concentrations of bortezomib for 48 h (16). Untreated cells were used as controls. The percentages and the corresponding significance levels of cells in various cell cycle phases are listed in Table II. Representative flow cytometry plots of untreated chondrosarcoma cells and those irradiated with 20 Gy are presented in Fig. 2A, and plots of the corresponding bortezomib-treated groups are presented in Fig. 2B. The graphical representations of the G0/G1, S and G2/M values are shown in stacked bars. High-dose IR induced a significant increase in the number of cells in the G2/M phase compared with that in the control group, which was accompanied by a decrease in the number of G1 and S phase cells, indicating a persisting G2/M phase arrest at 48 h post-IR. Notably, a co-treatment with bortezomib reduced the G2/M phase arrest.

Table II.

Cell cycle distribution of chondrosarcoma cells 48 h after 5 and 20 Gy IR.

	Cell cycle distribution at 48 h, %
Group	G1	S	G2/M
Ctrl	55.9±7.8	23.6±6.1	20.8±6.7
Ctrl + 5 Gy IR	52.5±13.5	17.1±5.8	30.4±9.1
Ctrl + 20 Gy IR	24.5±2.7[a]	8.9±3.1[a]	66.5±4.5[a]
Bor	52.9±7.8	26.5±6.2	20.5±5.3
Bor + 5 Gy IR	57.9±6.6	16.6±4.6[b]	25.5±10.1
Bor + 20 Gy IR	47.5±9.7[c]	7.5±1.4[a]	45.0±9.2[a,d]

P<0.001

P=0.026

P=0.021 vs. ctrl + 20 Gy IR

P=0.006 vs. ctrl + 20 Gy IR; n=5; one-way ANOVA with Holm-Sidak test for multiple comparisons. Data are presented as the mean ± SD. IR, ionizing radiation; Ctrl, control; Bor, bortezomib.

Figure 2.

Effects of bortezomib and IR on the cell cycle distribution. (A and B) Representative flow cytometry cell cycle measurements (A) at 48 h post-IR exposure and (B) following co-treatment with bortezomib. Cell populations in the G0/G1, S and G2/M phases are presented as the mean percentage of total cells (n=5). IR caused a decrease in the number of cells in G1 (black) and S (dashed) phase, which was accompanied by a pronounced increase of cells in G2/M phase (grey) (n=5). IR, ionizing radiation; Ctrl, control; Bor, bortezomib.

Induction of apoptosis by bortezomib and IR treatment

In order to investigate a possible sensitization to IR of chondrosarcoma cells by pretreatment with bortezomib, RT-qPCR was performed for the detection of the relative expression levels of the proapoptotic markers Bax and Bak, the antiapoptotic marker Bcl-2, the proapoptotic antagonist Noxa and the death receptor TRAIL-R2 (Fig. 3A). The mRNA levels of the proapoptotic genes Bax and Bak were significantly increased by bortezomib treatment compared with those in the control group. IR achieved higher or similar levels of Bax and Bak, respectively, compared with those in bortezomib-treated cells. Notably, co-treatment with bortezomib and IR decreased the Bax and Bak mRNA levels compared with those in cells treated with IR alone. By contrast, bortezomib reduced the Bcl-2 and Noxa mRNA levels in non-irradiated cells, whereas in IR-treated cells, bortezomib significantly enhanced the 5 Gy-induced effect. In addition, IR increased the protein expression of TRAIL-R2 by 2-fold (Fig. 3B). Annexin V/PI flow cytometric measurements revealed only a limited increase in the Annexin+/PI− cells between the untreated and the co-treatment groups Fig. 3C). In addition, there was no significant increase in early or late apoptotic cells between the control and Bor + 20 Gy groups. The lack of apoptotic induction was further verified with a PARP/cleaved PARP immunoblot (Fig. 3D).

Figure 3.

Effects of bortezomib and IR on apoptotic induction. (A) mRNA expression analysis of pro- and antiapoptotic markers was performed at 48 h post-IR. The expression levels of the proapoptotic markers Bax and Bak were significantly increased by bortezomib treatment compared with those in the control group, and further enhanced by co-treatment with IR. In addition, bortezomib reduced the mRNA expression levels of Bcl-2 and Noxa compared with those in the control group. IR increased the relative expression levels of TRAIL-R2 (n=5, mean ± SEM). (B) Western blot analyses were used to verify the results at the protein level. β-actin was used as loading control (n=3). (C) Annexin V/PI flow cytometric analysis at 48 h identified no significant changes following treatment with bortezomib or IR (n=3). Representative measurements of the control and bortezomib + 20 Gy groups are presented. (D) The PARP/cleaved PARP immunoblotting revealed no apoptotic induction. (E) Western blot analysis of the autophagy marker LC3BI–II in SW1353 cells treated with 5 and 10 nM bortezomib for 24 h. Lysosomal protease inhibitors blocked the autophagic flux and inhibited the degradation of LC3B-II. Additional IR induced no changes. β-actin was used as the loading control (n=2). IR, ionizing radiation; Ctrl, control; Bor, bortezomib; PARP, poly (ADP-ribose) polymerase; TRAIL-R2, TNF-related apoptosis-inducing ligand receptor 2; LC3, microtubule-associated protein 1A/1B-light chain 3; Inh, E64d and pepstatin A.

To determine the potential interplay between apoptosis and autophagy, immunoblotting of LC3B was performed. As demonstrated in Fig. 3E, increasing concentrations of bortezomib significantly increased the expression levels of LC3B-II compared with those in the untreated cells. The lysosomal protease inhibitors E64d and pepstatin A inhibited the degradation of LC3B-II. IR, however, did not increase the expression of LC3B-II.

Expression of MMPs following bortezomib and IR treatment

Chondrosarcoma spheroid cultures were irradiated with 5 Gy following pretreatment with bortezomib, and total RNA was isolated and analyzed by qPCR. The results revealed that treatment with bortezomib or IR alone and the combined treatment significantly increased the expression levels of MMP2 and MMP9 compared with those in the control cells (Fig. 4A).

Figure 4.

Signaling pathways affected by bortezomib and IR. (A) Bortezomib treatment significantly increased the mRNA expression levels of MMP2 and MMP9 compared with those in the control group, whereas irradiation exerted only a minor effect (n=5, mean ± SEM). (B) mRNA expression analysis of survivin was performed at 48 h post-IR. A synergistic decrease in the survivin expression levels was observed when bortezomib and IR were combined. (C) Western blot analyses of survivin and its downstream proteins were used to verify the results at the protein level. β-actin was used as loading control (n=3, mean ± SEM). IR, ionizing radiation; Ctrl, control; Bor, bortezomib; MMP, matrix metalloproteinase; HSP90, heat shock protein 90; XIAP, X-linked inhibitor of apoptosis protein.

Synergistic effects of bortezomib and IR on the survivin signaling pathway

Following bortezomib treatment and IR, RNA was isolated from the cells, and the relative expression of the BIRC5 gene was determined using RT-qPCR. The expression levels of BIRC5 mRNA were significantly decreased by both bortezomib and IR treatment compared with those in the control group. In addition, co-treatment with bortezomib and IR resulted in an additive reduction (Fig. 4B). Western blot analyses of survivin and its downstream proteins were used to verify these results at the protein level (Fig. 4C). Compared with those in the control cells, treatment with bortezomib and IR reduced the protein expression levels of HSP90 in chondrosarcoma spheroid cultures, and the expression levels of XIAP, Smad2 and Smad3 were reduced by IR (Fig. 4C).

DNA repair mechanisms following treatment with bortezomib and IR

To determine the potential involvement of bortezomib in radiation therapy resistance, DNA damage and the corresponding DNA repair genes were studied. The results demonstrated that at 24 h post-IR, not only the HDR, but also the NER pathway is positively regulated compared with those in the untreated controls (Fig. 5A). The number of double-strand breaks was determined by quantifying the levels of γH2AX at 1 and 24 h post-bortezomib pretreatment and IR (Fig. 5B). Chondrosarcoma cells exhibited a significant upregulation in γH2AX levels compared with those in the untreated control cells at 1 h post-IR independent of bortezomib, whereas at 24 h post-IR, the levels of this indirect marker of DNA damage returned to the baseline. These observations were confirmed by immunohistochemical staining (Fig. 5C) and indicated rapid and efficient DNA damage repair in chondrosarcoma cells.

Figure 5.

Effects of bortezomib and IR on DNA damage. (A) Heatmap of RNA sequencing data of the key genes involved in DNA repair mechanism pathways. The results are presented as fold-change compared with the untreated controls (n=3). (B) Following 24-h pretreatment with bortezomib and an additional 1 h post-IR, chondrosarcoma cells exhibited a significant increase in γH2AX protein phosphorylation levels compared with those in the control group. At 24 h post-IR, this DNA damage marker returned to baseline levels. (C) Immunohistochemical staining of γH2AX at 1 and 24 h post-IR. IR, ionizing radiation; Ctrl, control; Bor, bortezomib; γH2AX, histone H2AX phosphorylation at serine 139.

Discussion

In patients with chondrosarcoma with unfavorable initial characteristics, a high grade and incomplete resection, postoperative radiotherapy is an expedient option; it reduces the risk of local recurrence and improves 5-year disease-free survival from 64.2 to 71.8% (22). Chondrosarcomas exhibit variable response to irradiation, possibly due to their strong genetic heterogeneity, which is also evident in different chondrosarcoma cell lines (23,24). In this context, it is particularly important to gain new insights into the underlying tumor biology of chondrosarcoma. The use of 3D spheroid cultures represents the in vivo environment more accurately compared with conventional 2D monolayer cultures; 3D cultivation mimics the architecture of the donor tissue and enables both cell-cell and cell-matrix interactions (25). Therefore, the present study utilized the hanging drop 3D spheroid model. The viability of the two cell types used in the present study was only moderately affected by increasing IR intensity. Co-treatment with bortezomib appeared to be the main trigger for the observed decrease in the viability of chondrosarcoma cells. During radiotherapy, the co-treatment may positively affect the therapeutic efficacy of 5 Gy IR.

DNA structure checkpoints are necessary in the regulation of the cell cycle; they cause cell cycle arrest in response to incomplete replication or DNA damage. Known as the ‘restriction point’ in human cells, this point is defined as the moment after which cells are committed to entering and progressing through the cell cycle independent of signals from the environment (26). The G2/M phase delay in tumor cells following IR enables sufficient time for repair processes and ensures cell survival of otherwise lethal DNA damage (27). A previous study has reported that X-ray irradiation induces a G2/M phase arrest in HeLa cancer cells and is associated with the increase of the expression levels of checkpoint kinase 1 and the reduction of those of cyclin-dependent kinase 1 (28,29). The results of the present study revealed that both untreated and bortezomib pretreated spheroid cultures exhibited G2/M accumulation following IR. This replication arrest may enable the chondrosarcoma cells to regenerate more efficiently. The changes in cell cycle distribution observed in other cell systems following bortezomib treatment alone, however, were not be detected in chondrosarcoma cells in the present study (30,31).

An important indicator of possible damage in tumor cells is the induction of apoptosis. Bcl-2 family proteins exert undergo pro- or anti-apoptotic activities and regulate the mitochondrial apoptosis pathway by controlling outer mitochondrial membrane permeability (32). Reacting to a variety of types of damage or stress, certain Bcl-2 family members induce Bax or Bak proapoptotic protein activation at the mitochondrion (32). Bax and Bak homo-oligomerize and function in the outer mitochondrial membrane in pore formation, via which proapoptotic molecules escape; this results in the development of the morphological characteristics of apoptosis, including DNA laddering, condensed nuclei and phosphatidylserine exposure to the outer plasma membrane leaflet (33). In the present study, in chondrosarcoma cells, both bortezomib treatment and IR increased the expression levels of Bax and Bak compared with those in the control group. Notably, the combination of the two treatments did not lead to a synergistic enhancement of these effects, whereas the reduction in the expression levels of the antiapoptotic protein Bcl-2 was significantly enhanced by the co-treatment with IR and bortezomib. This was particularly notable at the protein level. Noxa is an indirect activator of apoptosis, as it only neutralizes the prosurvival members of the Bcl-2 family Mcl1 and Bcl-2, preventing them from regulating the levels of Bax and Bak (34). The levels of Noxa were also significantly decreased in the spheroid cultures by co-treatment with IR and bortezomib compared with those in the control cells in the present study. As a tumor necrosis factor receptor superfamily protein, which is mainly localized in the cell membrane, TRAIL-R2 triggers caspase-8 activation in response to anticancer drug treatment (35). The irradiation intensity of 5 Gy increased the protein expression levels of TRAIL-R2 2-fold compared what that in the controls. Despite the regulation of these apoptotic genes, only a small increase in the percentage of late apoptotic cells (Annexin+/PI−) was observed 48 h after IR and/or bortezomib treatment.

Autophagy has been discussed in the literature in recent years as an alternative cell death mechanism to apoptosis (36,37). During autophagy, the microtubule-associated protein LC3B is localized on the membranes, resulting in autophagosome membrane formation, and the level of LC3B-II is a marker for autophagosome formation (38). Bortezomib increases the autophagy-associated protein expression levels, including that of as autophagy-related 5/12, LC3I–II and Beclin in chondrosarcoma cells (16). However, an additional increase in the levels of autophagy-associated proteins by IR was not observed in the present study.

MMP2 and MMP9 are cancer-associated, secreted, zinc-dependent endopeptidases, the secretion of which is elevated in several types of human cancer such as liposarcoma, cervical, pancreatic or prostate cancer; their elevated expression has been associated with a poor prognosis (39). In the present study, bortezomib treatment significantly increased the expression levels of MMP2 and MMP9 compared with those in the untreated control cells, whereas irradiation exerted limited effects.

The results of the present study demonstrated the synergistic effects of bortezomib and IR on the mRNA and protein levels of survivin. Survivin is the smallest member of the inhibitor of apoptosis protein family (IAPs), and serves vital roles in the regulation of cell division and the inhibition of apoptosis via caspase activation blockade (40,41). Survivin has been highlighted vital for chondrosarcoma survival using small interfering RNA screening for 51 apoptosis-related genes in chondrosarcoma cells (42). The molecular chaperone Hsp90 participates in importing various client proteins to the mitochondria and has been demonstrated to be associated with survivin and other IAPs (43). Compared with that in the control cells, treatment with bortezomib and IR reduced the protein expression of HSP90 in chondrosarcoma spheroid cultures and thus may have impeded the survivin pathway. As a potent inhibitor of apoptosis, XIAP interacts directly with caspases to inhibit them (44). The reduced expression levels of survivin and XIAP caused by IR in the present study may lead to the induction of apoptosis. TGF-β and its signaling effectors smad 2 and smad 3 are key factors in controlling tumor cell proliferation and differentiation (45), and repress survivin expression at the transcriptional level (46). Both smad 2 and smad 3 exhibited a significant reduction in their protein expression levels following IR compared with those in the control group. Thus, the inhibition of the entire survivin pathway was demonstrated, especially after IR, in the present study.

Another important aspect of cancer therapy is the regeneration behavior of tumor cells after radiotherapy. Radiation-induced cell death mostly occurs due to DNA damage, especially due to double-strand breaks (47). The gene expression profiling data in the present study revealed that, supplementary to the HDR pathway, as described in the literature for other types of cancer such as breast or ovarian cancer (48), the NER pathway, with the key players excision repair cross-complementation group 1 and 5, is also activated in chondrosarcoma cells after IR. Although cell viability was decreased both after treatment with bortezomib and after irradiation compared with that in untreated cells, increased DNA repair following treatment was observed in the present study. Since the levels of γH2AX are associated with DNA double-strand breaks, it is the most common surrogate marker to study DNA damage induction and the subsequent repair of the DNA lesion (49). In the present study, comparative analysis at 1 and 24 h post-IR indicated efficient DNA repair in human chondrosarcoma cells, returning to baseline levels within 24 h.

In conclusion, the results of the present study demonstrated that co-treatment with bortezomib improved the radiation sensitivity of chondrosarcoma cells to a limited extent. The inhibition of the survivin signaling pathway revealed a novel interesting aspect in the tumor biology of chondrosarcoma 3D spheroid cultures and may provide a future option for clinical exploitation.