A W Kashif1, Nisha Verma2, Shipra Verma2, Dibyajyoti Boruah3, Rajesh Sahu4, Suprita Kalra5, Ajay Malik6. 1. Assistant Professor, Department of Pathology, Armed Forces Medical College, India. 2. Resident, Department of Pathology, Armed Forces Medical College, India. 3. Scientist 'E', Department of Pathology, Armed Forces Medical College, India. 4. Assistant Professor, Department of Community Medicine, Armed Forces Medical College, India. 5. Classified Specialist (Paediatrics), Army Hospital (R&R), New Delhi, India. 6. Brig Med, (Delhi Area), India.
Abstract
BACKGROUND: Morphometry has now become a useful adjunct to the diagnostic armamentarium of light, immunofluorescence, and electron microscopy, as it provides a deep insight into quantitative parameters of nephropathies. There has been a limited study on its utility especially in diagnosing pediatric renal diseases. This study is probably the first in India to assess the contribution of this diagnostic modality in pediatric renal disease to the best of authors' knowledge. METHODS: It's a retrospective cross-sectional study covering a period of 05 years at a tertiary care hospital. The study includes 28 cases of pediatric (age till 14 years) nephropathies. The diseases were divided into two groups-nephrotic presentation and nephritic presentation. Glomerular morphometry was performed and mean was calculated for Bowman's capsule area, glomerular capillary tuft area, and Bowman's space area; for the three groups, respectively. Renal parameters serum creatinine, blood urea, 24 h urine protein were studied along with hemoglobin and serum cholesterol for the cases. Data were analyzed using SPSS software, version 25, for one-way ANOVA comparing mean in the three groups. RESULTS: We found a positive and significant correlation between Bowman's capsule area with proteinuria, blood urea, and serum creatinine. There was positive and significant correlation between glomerular capillary tuft area and serum creatinine and Bowman's space area and proteinuria in both the groups. CONCLUSION: Glomerular morphometry may contribute to the diagnosis of some glomerulopathies and the association between glomerular morphometric parameters and laboratory data may promote better understanding of the prognosis of these patients.
BACKGROUND: Morphometry has now become a useful adjunct to the diagnostic armamentarium of light, immunofluorescence, and electron microscopy, as it provides a deep insight into quantitative parameters of nephropathies. There has been a limited study on its utility especially in diagnosing pediatric renal diseases. This study is probably the first in India to assess the contribution of this diagnostic modality in pediatric renal disease to the best of authors' knowledge. METHODS: It's a retrospective cross-sectional study covering a period of 05 years at a tertiary care hospital. The study includes 28 cases of pediatric (age till 14 years) nephropathies. The diseases were divided into two groups-nephrotic presentation and nephritic presentation. Glomerular morphometry was performed and mean was calculated for Bowman's capsule area, glomerular capillary tuft area, and Bowman's space area; for the three groups, respectively. Renal parameters serum creatinine, blood urea, 24 h urine protein were studied along with hemoglobin and serum cholesterol for the cases. Data were analyzed using SPSS software, version 25, for one-way ANOVA comparing mean in the three groups. RESULTS: We found a positive and significant correlation between Bowman's capsule area with proteinuria, blood urea, and serum creatinine. There was positive and significant correlation between glomerular capillary tuft area and serum creatinine and Bowman's space area and proteinuria in both the groups. CONCLUSION: Glomerular morphometry may contribute to the diagnosis of some glomerulopathies and the association between glomerular morphometric parameters and laboratory data may promote better understanding of the prognosis of these patients.
Authors: Wendy E Hoy; John F Bertram; Rebecca Douglas Denton; Monika Zimanyi; Terence Samuel; Michael D Hughson Journal: Curr Opin Nephrol Hypertens Date: 2008-05 Impact factor: 2.894
Authors: Bridgette J McNamara; Boucar Diouf; Michael D Hughson; Wendy E Hoy; John F Bertram Journal: Nephrol Dial Transplant Date: 2008-11-21 Impact factor: 5.992
Authors: Siddharth Tripathi; A W Kashif; Ajay Malik; Dibyajyoti Boruah; Rajesh Sahu; S K Panda; Gourang Paliwal Journal: Med J Armed Forces India Date: 2021-10-21