Literature DB >> 33867559

Anomalous differential polarized phase angles for two-photon excitation with isotropic depolarizing rotations.

Joseph R Lakowicz1, Ignacy Gryczynski1, Eva Danielsen1.   

Abstract

We describe frequency-domain measurements of the anisotropy decay of 1,6-diphenylhexatriene resulting from one- and two-photon excitation. For two-photon excitation, the phase shifts (Δ) between the horizontally and vertically polarized components of the decay exceed the absolute maximum of 30° possible for one-photon excitation, and values of Δ as large as 37° were observed for 1,6-diphenylhexatriene in triacetin. These results are explained by the increased orientation of the photoselected population for two- as compared to one-photon excitation.

Entities:  

Year:  2002        PMID： 33867559      PMCID： PMC8049530          DOI： 10.1016/0009-2614(92)85366-i

Source DB:  PubMed          Journal:  Chem Phys Lett        ISSN： 0009-2614            Impact factor:   2.328


  10 in total

1.  Anisotropy decays of indole, melittin monomer and melittin tetramer by frequency-domain fluorometry and multi-wavelength global analysis.

Authors:  J R Lakowicz; I Gryczynski; H Cherek; G Laczko
Journal:  Biophys Chem       Date:  1991-03       Impact factor: 2.352

2.  Anisotropy decays of single tryptophan proteins measured by GHz frequency-domain fluorometry with collisional quenching.

Authors:  J R Lakowicz; I Gryczynski; H Szmacinski; H Cherek; N Joshi
Journal:  Eur Biophys J       Date:  1991       Impact factor: 1.733

3.  Resolution of the fluorescence excitation spectrum of indole into the 1La and 1Lb excitation bands.

Authors:  B Valeur; G Weber
Journal:  Photochem Photobiol       Date:  1977-05       Impact factor: 3.421

4.  Orientational exchange approach to fluorescence anisotropy decay.

Authors:  D W Piston; E Gratton
Journal:  Biophys J       Date:  1989-12       Impact factor: 4.033

5.  Resolution of complex anisotropy decays by variable frequency phase-modulation fluorometry: a stimulation study.

Authors:  B P Maliwal; J R Lakowicz
Journal:  Biochim Biophys Acta       Date:  1986-09-26

6.  Enhanced resolution of fluorescence anisotropy decays by simultaneous analysis of progressively quenched samples. Applications to anisotropic rotations and to protein dynamics.

Authors:  J R Lakowicz; H Cherek; I Gryczynski; N Joshi; M L Johnson
Journal:  Biophys J       Date:  1987-05       Impact factor: 4.033

7.  Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry.

Authors:  J R Lakowicz; H Cherek; B P Maliwal
Journal:  Biochemistry       Date:  1985-01-15       Impact factor: 3.162

8.  A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.

Authors:  E Gratton; M Limkeman
Journal:  Biophys J       Date:  1983-12       Impact factor: 4.033

9.  Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data.

Authors:  J R Lakowicz; G Laczko; H Cherek; E Gratton; M Limkeman
Journal:  Biophys J       Date:  1984-10       Impact factor: 4.033

10.  Determination of time-resolved fluorescence emission spectra and anisotropies of a fluorophore-protein complex using frequency-domain phase-modulation fluorometry.

Authors:  J R Lakowicz; E Gratton; H Cherek; B P Maliwal; G Laczko
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157
  10 in total

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