Literature DB >> 33867550

Evaluation of commercial probiotics for antimicrobial resistance genes.

Rachel M Baumgardner1, Ana Berreta1, Jamie J Kopper1.   

Abstract

The objective of this study was to determine if transferable antimicrobial resistance (AMR) genes are present in commercial animal probiotics. DNA was extracted from 50 probiotics, tested for the presence of bacterial DNA, and analyzed by polymerase chain reaction (PCR) for the presence of 8 transferrable AMR genes, including tetracycline, erythromycin, aminoglycoside, sulfonamide, and trimethoprim. Samples that were positive by PCR were confirmed by genome sequencing. Forty-seven (94%) products contained bacterial DNA. Of these, 97% contained at least 1 AMR gene, and 82% contained 2 or more. These results indicate that further evaluation of the risk for transmission of these AMR genes may be warranted. Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.

Entities:  

Year:  2021        PMID: 33867550      PMCID: PMC7953935     

Source DB:  PubMed          Journal:  Can Vet J        ISSN: 0008-5286            Impact factor:   1.008


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