Martha E García-Aguilera1, Eduardo Rodríguez de San Miguel2, Jocelyn Cruz-Pérez3, Lucinda Aguirre-Cruz3, Christian M Ramirez-Alfaro3, Nuria Esturau-Escofet4. 1. Universidad Nacional Autónoma de México, Instituto de Química, Ciudad Universitaria, 04510, Mexico City, Mexico. 2. Departamento de Química Analítica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 Cd., Mexico City, Mexico. 3. Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes sur 3877, 14269, Mexico City, Mexico. 4. Universidad Nacional Autónoma de México, Instituto de Química, Ciudad Universitaria, 04510, Mexico City, Mexico. nesturau@iquimica.unam.mx.
Abstract
INTRODUCTION: Brain death (BD) is the irreversible cessation of all functions of the entire brain, including the brainstem. Cerebrospinal fluid (CSF) is a biological liquid that circulates in brain and spine. Metabolomics is able to reveal the response of biological systems to diverse factors in a specific moment or condition. Therefore, the study of this neurological condition through metabolic profiling using high resolution Nuclear Magnetic Resonance (NMR) spectroscopy is important for understanding biochemical events. OBJECTIVES: The aim of the current study is to identify the metabolomics signature of BD using 1H-NMR spectroscopy in human CSF. METHODS: 1H-NMR spectroscopy has been employed for metabolomic untargeted analysis in 46 CSF samples: 22 control and 24 with BD. Spectral data were further subjected to multivariate analysis. RESULTS: Statistically significant multivariate models separated subject's samples with BD from controls and revealed twenty one discriminatory metabolites. The statistical analysis of control and BD subjects using Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) model resulted in R2X of 0.733 and Q2 of 0.635. An elevation in the concentration of statistically discriminant metabolites in BD was observed. CONCLUSION: This study identifies a metabolic signature associated with BD and the most relevant enriched selected metabolic pathways.
INTRODUCTION: Brain death (BD) is the irreversible cessation of all functions of the entire brain, including the brainstem. Cerebrospinal fluid (CSF) is a biological liquid that circulates in brain and spine. Metabolomics is able to reveal the response of biological systems to diverse factors in a specific moment or condition. Therefore, the study of this neurological condition through metabolic profiling using high resolution Nuclear Magnetic Resonance (NMR) spectroscopy is important for understanding biochemical events. OBJECTIVES: The aim of the current study is to identify the metabolomics signature of BD using 1H-NMR spectroscopy in human CSF. METHODS: 1H-NMR spectroscopy has been employed for metabolomic untargeted analysis in 46 CSF samples: 22 control and 24 with BD. Spectral data were further subjected to multivariate analysis. RESULTS: Statistically significant multivariate models separated subject's samples with BD from controls and revealed twenty one discriminatory metabolites. The statistical analysis of control and BD subjects using Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) model resulted in R2X of 0.733 and Q2 of 0.635. An elevation in the concentration of statistically discriminant metabolites in BD was observed. CONCLUSION: This study identifies a metabolic signature associated with BD and the most relevant enriched selected metabolic pathways.
Authors: A A DeSalles; H A Kontos; D P Becker; M S Yang; J D Ward; R Moulton; H D Gruemer; H Lutz; A L Maset; L Jenkins Journal: J Neurosurg Date: 1986-11 Impact factor: 5.115