Sergio López-García1, María P Pecci-Lloret2, Miguel R Pecci-Lloret2, Julia Guerrero-Gironés3, Francisco J Rodríguez-Lozano4, David García-Bernal5. 1. Cellular Therapy and Hematopoietic Transplant Research Group, Biomedical Research Institute of Murcia, Clinical University Hospital Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain. 2. Gerodontology and Special Care Dentistry Unit, Hospital Morales Meseguer, Medicine School, University of Murcia, 30100 Murcia, Spain. 3. Gerodontology and Special Care Dentistry Unit, Hospital Morales Meseguer, Medicine School, University of Murcia, 30100 Murcia, Spain. Electronic address: julia.guerrero@um.es. 4. Cellular Therapy and Hematopoietic Transplant Research Group, Biomedical Research Institute of Murcia, Clinical University Hospital Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain; Gerodontology and Special Care Dentistry Unit, Hospital Morales Meseguer, Medicine School, University of Murcia, 30100 Murcia, Spain. 5. Cellular Therapy and Hematopoietic Transplant Research Group, Biomedical Research Institute of Murcia, Clinical University Hospital Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain; Internal Medicine Department, Medicine School, University of Murcia, 30100 Murcia, Spain.
Abstract
OBJECTIVE: To compare the in vitro cytotoxicity of four commercial topical fluoride varnishes widely used in daily dental practice for the prevention of caries on human fibroblasts: Cervitec F, Fixofluor, Fluor Protector S and Duraphat. MATERIAL AND METHODS: Human gingival fibroblasts (hGF) were exposed to different concentrations of fluoride varnishes extracts. Biological assays, including MTT and IC50 value determination, annexin-V/7-AAD staining, cell migration and F-actin staining with phalloidin were carried out. Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test. RESULTS: At 4% concentration, all of the fluoride varnishes extracts affected fibroblasts metabolic activity, exhibiting a high degree of cytotoxicity at all measured time points. At 0.1% and 1%, Duraphat and Fixofluor or Fluor Protector S and Cervitec F exerted the lowest or highest cytotoxic effects, respectively. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were analyzed. Immunocytochemical assays revealed a similar number of fibroblasts, without changes in the morphology and F-actin content at 0.1% concentration of all tested materials, while at 1% concentration, Fluor Protector S and Cervitec F showed few cells with aberrant morphology or non-adhered cells, respectively. CONCLUSIONS: Different commercial topical fluoride varnishes with the same therapeutic indication may exhibit different biological effects and cytotoxicity on fibroblasts.
OBJECTIVE: To compare the in vitro cytotoxicity of four commercial topical fluoride varnishes widely used in daily dental practice for the prevention of caries on human fibroblasts: Cervitec F, Fixofluor, Fluor Protector S and Duraphat. MATERIAL AND METHODS:Human gingival fibroblasts (hGF) were exposed to different concentrations of fluoride varnishes extracts. Biological assays, including MTT and IC50 value determination, annexin-V/7-AAD staining, cell migration and F-actin staining with phalloidin were carried out. Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test. RESULTS: At 4% concentration, all of the fluoride varnishes extracts affected fibroblasts metabolic activity, exhibiting a high degree of cytotoxicity at all measured time points. At 0.1% and 1%, Duraphat and Fixofluor or Fluor Protector S and Cervitec F exerted the lowest or highest cytotoxic effects, respectively. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were analyzed. Immunocytochemical assays revealed a similar number of fibroblasts, without changes in the morphology and F-actin content at 0.1% concentration of all tested materials, while at 1% concentration, Fluor Protector S and Cervitec F showed few cells with aberrant morphology or non-adhered cells, respectively. CONCLUSIONS: Different commercial topical fluoride varnishes with the same therapeutic indication may exhibit different biological effects and cytotoxicity on fibroblasts.
Authors: María Pilar Pecci-Lloret; Sergio López-García; Francisco Javier Rodríguez-Lozano; Pablo Álvarez-Novoa; David García-Bernal Journal: Int J Environ Res Public Health Date: 2022-03-03 Impact factor: 3.390