Literature DB >> 33855120

Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging.

Xiao Min Zhang1, Fabrice P Cordelieres2, Etienne Herzog3.   

Abstract

Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different "pools". Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named "SV super-pool". Here we describe a Fluorescent Recovery After Photobleaching (FRAP) approach to elucidate the relationship between SVs from the super-pool with SV clusters at presynaptic terminals. This method is powerful to investigate SV mobility regulation mechanisms.
Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Axonal transport; FRAP; Live-cell imaging; Membrane trafficking; Synapse; Synaptic vesicle

Year:  2021        PMID： 33855120      PMCID： PMC8032493          DOI： 10.21769/BioProtoc.3962

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  14 in total

1.  The use of pHluorins for optical measurements of presynaptic activity.

Authors:  S Sankaranarayanan; D De Angelis; J E Rothman; T A Ryan
Journal:  Biophys J       Date:  2000-10       Impact factor: 4.033

2.  Protonation, photobleaching, and photoactivation of yellow fluorescent protein (YFP 10C): a unifying mechanism.

Authors:  Tim B McAnaney; Wei Zeng; Camille F E Doe; Nina Bhanji; Stuart Wakelin; David S Pearson; Paul Abbyad; Xinghua Shi; Steven G Boxer; Clive R Bagshaw
Journal:  Biochemistry       Date:  2005-04-12       Impact factor: 3.162

3.  The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones.

Authors:  Cordelia Imig; Sang-Won Min; Stefanie Krinner; Marife Arancillo; Christian Rosenmund; Thomas C Südhof; JeongSeop Rhee; Nils Brose; Benjamin H Cooper
Journal:  Neuron       Date:  2014-10-22       Impact factor: 17.173

4.  Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly.

Authors:  Tolga Soykan; Natalie Kaempf; Takeshi Sakaba; Dennis Vollweiter; Felix Goerdeler; Dmytro Puchkov; Natalia L Kononenko; Volker Haucke
Journal:  Neuron       Date:  2017-02-22       Impact factor: 17.173

5.  The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse.

Authors:  D M Landis; A K Hall; L A Weinstein; T S Reese
Journal:  Neuron       Date:  1988-05       Impact factor: 17.173

Review 6.  The synaptic vesicle cycle: a cascade of protein-protein interactions.

Authors:  T C Südhof
Journal:  Nature       Date:  1995-06-22       Impact factor: 49.962

7.  Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.

Authors:  D Axelrod; D E Koppel; J Schlessinger; E Elson; W W Webb
Journal:  Biophys J       Date:  1976-09       Impact factor: 4.033

8.  Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins.

Authors:  Kou Qin; Pooja R Sethi; Nevin A Lambert
Journal:  FASEB J       Date:  2008-04-23       Impact factor: 5.191

9.  Synaptic vesicle pools: an update.

Authors:  Annette Denker; Silvio O Rizzoli
Journal:  Front Synaptic Neurosci       Date:  2010-10-05

10.  A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency.

Authors:  Urielle François; Kätlin Silm; Xiao Min Zhang; Maria Florencia Angelo; Maria Victoria Fernandez-Busch; Mona Maged; Christelle Martin; Véronique Bernard; Fabrice P Cordelières; Melissa Deshors; Stéphanie Pons; Uwe Maskos; Alexis Pierre Bemelmans; Sonja M Wojcik; Salah El Mestikawy; Yann Humeau; Etienne Herzog
Journal:  Elife       Date:  2019-10-30       Impact factor: 8.140
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