| Literature DB >> 33854272 |
Abstract
Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented.Entities:
Keywords: FLIM; Fourier series; IRF; SPAD; lifetime; phasor; single-exponential decay; time-gate
Year: 2021 PMID: 33854272 PMCID: PMC8041354 DOI: 10.1117/12.2577747
Source DB: PubMed Journal: Proc SPIE Int Soc Opt Eng ISSN: 0277-786X