Literature DB >> 33854272

An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays.

X Michalet1.   

Abstract

Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented.

Entities:  

Keywords:  FLIM; Fourier series; IRF; SPAD; lifetime; phasor; single-exponential decay; time-gate

Year:  2021        PMID: 33854272      PMCID: PMC8041354          DOI: 10.1117/12.2577747

Source DB:  PubMed          Journal:  Proc SPIE Int Soc Opt Eng        ISSN: 0277-786X


  16 in total

1.  Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data.

Authors:  A H A Clayton; Q S Hanley; P J Verveer
Journal:  J Microsc       Date:  2004-01       Impact factor: 1.758

Review 2.  Time-resolved fluorescence microscopy.

Authors:  Klaus Suhling; Paul M W French; David Phillips
Journal:  Photochem Photobiol Sci       Date:  2004-11-11       Impact factor: 3.982

Review 3.  Imaging protein molecules using FRET and FLIM microscopy.

Authors:  Horst Wallrabe; Ammasi Periasamy
Journal:  Curr Opin Biotechnol       Date:  2005-02       Impact factor: 9.740

4.  Polar plot representation for frequency-domain analysis of fluorescence lifetimes.

Authors:  Glen I Redford; Robert M Clegg
Journal:  J Fluoresc       Date:  2005-09       Impact factor: 2.217

Review 5.  Fluorescence lifetime imaging microscopy (FLIM).

Authors:  Erik B van Munster; Theodorus W J Gadella
Journal:  Adv Biochem Eng Biotechnol       Date:  2005       Impact factor: 2.635

6.  The phasor approach to fluorescence lifetime imaging analysis.

Authors:  Michelle A Digman; Valeria R Caiolfa; Moreno Zamai; Enrico Gratton
Journal:  Biophys J       Date:  2007-11-02       Impact factor: 4.033

7.  A novel fluorescence lifetime imaging system that optimizes photon efficiency.

Authors:  Ryan A Colyer; Claudia Lee; Enrico Gratton
Journal:  Microsc Res Tech       Date:  2008-03       Impact factor: 2.769

8.  Global analysis of time correlated single photon counting FRET-FLIM data.

Authors:  Hernan E Grecco; Pedro Roda-Navarro; Peter J Verveer
Journal:  Opt Express       Date:  2009-04-13       Impact factor: 3.894

9.  Fit-free analysis of fluorescence lifetime imaging data using the phasor approach.

Authors:  Suman Ranjit; Leonel Malacrida; David M Jameson; Enrico Gratton
Journal:  Nat Protoc       Date:  2018-09       Impact factor: 13.491

10.  Continuous and discrete phasor analysis of binned or time-gated periodic decays.

Authors:  Xavier Michalet
Journal:  AIP Adv       Date:  2021-03-23       Impact factor: 1.548

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