Ida Kokalari1, Sandeep Keshavan2, Mizanur Rahman2, Elena Gazzano3, Giulia Barzan4,5, Luisa Mandrile4, Andrea Giovannozzi4, Jessica Ponti6, Giulia Antonello1, Marco Monopoli7, Guido Perrone8, Enrico Bergamaschi9, Chiara Riganti10, Bengt Fadeel2, Ivana Fenoglio1. 1. Department of Chemistry, University of Torino, 10125 Torino, Italy. 2. Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, 17177 Stockholm, Sweden. 3. Department of Life Sciences & Systems Biology, University of Torino, 10123 Torino, Italy. 4. National Institute of Metrological Research (INRiM), 10135 Torino, Italy. 5. Department of Electronics andTelecommunications, Politecnico di Torino, 10129 Turin, Italy. 6. EuropeanCommission, Joint Research Centre (JRC), 21027 Ispra (VA), Italy. 7. Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland. 8. Department of Electronics & Telecommunications, Polytechnic of Torino, 10129 Torino, Italy. 9. Department of Public Health & Pediatrics, University of Torino, 10126 Torino, Italy. 10. Department of Oncology, University of Torino, 10126 Torino, Italy.
Abstract
Aim: To investigate near infrared-induced phototoxicity toward lung cancer cells, and the biodegradability and effect on immune cells of glucose-derived carbon nanoparticles (CNPs). Methods: The human A549 lung adenocarcinoma cell line was used as a model to study the phototoxicity of CNPs. The biodegradability and the effect on immune cells was demonstrated in primary human neutrophils and macrophages. Results: Near infrared-activated CNPs elicited rapid cell death, characterized by the elevation of heat shock proteins and the induction of DNA damage. CNPs were found to be noncytotoxic toward primary human macrophages and were susceptible to biodegradation when cocultured with human neutrophils. Conclusions: Our results identify CNPs as promising platforms for photothermal therapy of lung cancer.
Aim: To investigate near infrared-induced phototoxicity toward lung cancer cells, and the biodegradability and effect on immune cells of glucose-derived carbon nanoparticles (CNPs). Methods: The humanA549lung adenocarcinoma cell line was used as a model to study the phototoxicity of CNPs. The biodegradability and the effect on immune cells was demonstrated in primary human neutrophils and macrophages. Results: Near infrared-activated CNPs elicited rapid cell death, characterized by the elevation of heat shock proteins and the induction of DNA damage. CNPs were found to be noncytotoxic toward primary human macrophages and were susceptible to biodegradation when cocultured with human neutrophils. Conclusions: Our results identify CNPs as promising platforms for photothermal therapy of lung cancer.
Authors: Giulia Barzan; Ida Kokalari; Giacomo Gariglio; Elena Ghibaudi; Marc Devocelle; Marco P Monopoli; Alessio Sacco; Angelo Greco; Andrea M Giovannozzi; Andrea M Rossi; Ivana Fenoglio Journal: ACS Omega Date: 2022-05-05