Nayab Fatima1, Ugur Akcan2, Mehmet Kaya2, Radka Gromnicova1, Jane Loughlin1, Basil Sharrack3, David Male1. 1. Department of Life, Health & Chemical Sciences, The Open University, Milton Keynes, UK. 2. Department of Physiology, Koç University School of Medicine, Istanbul, Turkey. 3. Academic Department of Neuroscience & Sheffield, NIHR Translational Neuroscience BRC, Sheffield Teaching Hospitals, NHS Foundation Trust, University of Sheffield, Sheffield, UK.
Abstract
Aim: The aim of the study was to determine how the addition of a DNA oligonucleotide cargo to 3-nm gold glyconanoparticles would affect tissue distribution. Methods: Gold glyconanoparticles with 1-6 covalently bound oligonucleotides (40 nt dsDNA) were injected into rats and allowed to circulate for 10 min. Organs were harvested and gold quantitated by inductively coupled plasma mass spectrometry. Cellular localization of the nanocarriers was determined by electron microscopy. Results and Conclusion: Addition of DNA cargo to the nanocarriers prevented localization in the kidney but increased localization in liver hepatocytes and splenic macrophages. There was no significant change in heart, lung or brain. DNA increases the size and adds a strong negative charge to the nanoparticles, which radically affects tissue distribution.
Aim: The aim of the study was to determine how the addition of a DNA oligonucleotide cargo to 3-nm gold glyconanoparticles would affect tissue distribution. Methods:Gold glyconanoparticles with 1-6 covalently bound oligonucleotides (40 nt dsDNA) were injected into rats and allowed to circulate for 10 min. Organs were harvested and gold quantitated by inductively coupled plasma mass spectrometry. Cellular localization of the nanocarriers was determined by electron microscopy. Results and Conclusion: Addition of DNA cargo to the nanocarriers prevented localization in the kidney but increased localization in liver hepatocytes and splenic macrophages. There was no significant change in heart, lung or brain. DNA increases the size and adds a strong negative charge to the nanoparticles, which radically affects tissue distribution.
Entities:
Keywords:
DNA oligonucleotides; brain; electron microscopy; gold nanoparticles; kidney; liver; spleen; tissue distribution