Omry Koren1, Avi Peretz2,3, Dana Binyamin1, Orna Nitzan4, Maya Azrad5, Zohar Hamo5. 1. The Azrieli Faculty of Medicine, Bar Ilan University, 1311502, Safed, Israel. 2. The Azrieli Faculty of Medicine, Bar Ilan University, 1311502, Safed, Israel. aperetz@poria.health.gov.il. 3. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Hanna Senesh 818/2, 15208, Poriya, Tiberias, Israel. aperetz@poria.health.gov.il. 4. Unit of Infectious Diseases, Baruch Padeh Medical Center, 15208, Poriya, Israel. 5. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Hanna Senesh 818/2, 15208, Poriya, Tiberias, Israel.
Abstract
BACKGROUND: Clostridioides difficile (C. difficile) is a major nosocomial pathogen that infects the human gut and can cause diarrheal disease. A dominant risk factor is antibiotic treatment that disrupts the normal gut microbiota. The aim of the study was to examine the correlation between antibiotic treatment received prior to C. difficile infection (CDI) onset and patient gut microbiota. METHODS: Stool samples were collected from patients with CDI, presenting at the Baruch Padeh Medical Center Poriya, Israel. Demographic and clinical information, including previous antibiotic treatments, was collected from patient charts, and CDI severity score was calculated. Bacteria were isolated from stool samples, and gut microbiome was analyzed by sequencing the 16S rRNA gene using the Illumina MiSeq platform and QIIME2. RESULTS: In total, 84 patients with CDI were enrolled in the study; all had received antibiotics prior to disease onset. Due to comorbidities, 46 patients (55%) had received more than one class of antibiotics. The most common class of antibiotics used was cephalosporins (n = 44 cases). The intestinal microbiota of the patients was not uniform and was mainly dominated by Proteobacteria. Differences in intestinal microbiome were influenced by the different combinations of antibiotics that the patients had received (p = 0.022) CONCLUSIONS: The number of different antibiotics administered has a major impact on the CDI patients gut microbiome, mainly on bacterial richness.
BACKGROUND:Clostridioides difficile (C. difficile) is a major nosocomial pathogen that infects the human gut and can cause diarrheal disease. A dominant risk factor is antibiotic treatment that disrupts the normal gut microbiota. The aim of the study was to examine the correlation between antibiotic treatment received prior to C. difficileinfection (CDI) onset and patient gut microbiota. METHODS: Stool samples were collected from patients with CDI, presenting at the Baruch Padeh Medical Center Poriya, Israel. Demographic and clinical information, including previous antibiotic treatments, was collected from patient charts, and CDI severity score was calculated. Bacteria were isolated from stool samples, and gut microbiome was analyzed by sequencing the 16S rRNA gene using the Illumina MiSeq platform and QIIME2. RESULTS: In total, 84 patients with CDI were enrolled in the study; all had received antibiotics prior to disease onset. Due to comorbidities, 46 patients (55%) had received more than one class of antibiotics. The most common class of antibiotics used was cephalosporins (n = 44 cases). The intestinal microbiota of the patients was not uniform and was mainly dominated by Proteobacteria. Differences in intestinal microbiome were influenced by the different combinations of antibiotics that the patients had received (p = 0.022) CONCLUSIONS: The number of different antibiotics administered has a major impact on the CDI patientsgut microbiome, mainly on bacterial richness.
Entities:
Keywords:
Clostridioides difficile; Gut microbiome; Infection
Authors: William Sangster; John P Hegarty; Kathleen M Schieffer; Justin R Wright; Jada Hackman; David R Toole; Regina Lamendella; David B Stewart Journal: Front Microbiol Date: 2016-05-25 Impact factor: 5.640