Literature DB >> 3384936

Chromatofocusing in the purification of staphylococcal enterotoxin D.

Z Lei1, R F Reiser, M S Bergdoll.   

Abstract

A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.

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Year:  1988        PMID： 3384936      PMCID： PMC266573          DOI： 10.1128/jcm.26.6.1236-1237.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Production of enterotoxin a.

Authors:  E Kato; M Khan; L Kujovich; M S Bergdoll
Journal:  Appl Microbiol       Date:  1966-11

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Purification and some physicochemical properties of toxic-shock toxin.

Authors:  R F Reiser; R N Robbins; G P Khoe; M S Bergdoll
Journal:  Biochemistry       Date:  1983-08-02       Impact factor: 3.162

4.  Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.

Authors:  I A Ende; G Terplan; B Kickhöfen; D K Hammer
Journal:  Appl Environ Microbiol       Date:  1983-12       Impact factor: 4.792

5.  Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.

Authors:  R F Reiser; R N Robbins; A L Noleto; G P Khoe; M S Bergdoll
Journal:  Infect Immun       Date:  1984-09       Impact factor: 3.441

6.  Simple production of staphylococcal enterotoxins (SET) with chromatofocusing and isoelectrofocusing in flat bed gels.

Authors:  H Fey; H Pfister; C Müller
Journal:  Zentralbl Veterinarmed B       Date:  1984-08

7.  Detecting the enterotoxigenicity of Staphylococcus aureus strains.

Authors:  R Robbins; S Gould; M Bergdoll
Journal:  Appl Microbiol       Date:  1974-12

8.  Purification and some physicochemical properties of staphylococcal enterotoxin D.

Authors:  H C Chang; M S Bergdoll
Journal:  Biochemistry       Date:  1979-05-15       Impact factor: 3.162
  8 in total
  2 in total

1.  Improved purification and biologic activities of staphylococcal toxic shock syndrome toxin 1.

Authors:  W W Kum; K B Laupland; R H See; A W Chow
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

2.  Rapid purification of staphylococcal enterotoxin B by high-pressure liquid chromatography.

Authors:  M P Strickler; R J Neill; M J Stone; R E Hunt; W Brinkley; P Gemski
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948
  2 in total

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