Literature DB >> 33847947

Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications.

Alessandra Zarantonello1, Sofia Mortensen1, Nick S Laursen1, Gregers R Andersen2.   

Abstract

Activated complement component C4 (C4b) is the nonenzymatic component of the classical pathway (CP) convertases of the complement system. Preparation of C4 and C4b samples suitable for structural biology studies is challenging due to low yields and complexity of recombinant C4 production protocols reported so far and heterogeneity of C4 in native sources. Here we present a purification protocol for human C4 and describe sample preparation methods for structural investigation of C4 and its complexes by crystallography, small angle X-ray scattering, and electron microscopy.

Entities:  

Keywords:  Complement C4; Gel filtration; Ion exchange; Negative stain electron microscopy; Small angle X-ray scattering; X-ray crystallography

Year:  2021        PMID： 33847947     DOI： 10.1007/978-1-0716-1016-9_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

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Journal:  Immunopharmacology       Date:  2000-08

Review 2.  The internal thioester and the covalent binding properties of the complement proteins C3 and C4.

Authors:  S K Law; A W Dodds
Journal:  Protein Sci       Date:  1997-02       Impact factor: 6.725

Review 3.  Complement regulators and inhibitory proteins.

Authors:  Peter F Zipfel; Christine Skerka
Journal:  Nat Rev Immunol       Date:  2009-09-04       Impact factor: 53.106

4.  Structural Basis for the Function of Complement Component C4 within the Classical and Lectin Pathways of Complement.

Authors:  Sofia Mortensen; Rune T Kidmose; Steen V Petersen; Ágnes Szilágyi; Zoltan Prohászka; Gregers R Andersen
Journal:  J Immunol       Date:  2015-04-24       Impact factor: 5.422

5.  Structural basis for activation of the complement system by component C4 cleavage.

Authors:  Rune T Kidmose; Nick S Laursen; József Dobó; Troels R Kjaer; Sofia Sirotkina; Laure Yatime; Lars Sottrup-Jensen; Steffen Thiel; Péter Gál; Gregers R Andersen
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-04       Impact factor: 11.205

6.  Re-evaluation of low-resolution crystal structures via interactive molecular-dynamics flexible fitting (iMDFF): a case study in complement C4.

Authors:  Tristan Ian Croll; Gregers Rom Andersen
Journal:  Acta Crystallogr D Struct Biol       Date:  2016-08-18       Impact factor: 7.652

7.  Oligosaccharide structure of human C4.

Authors:  A C Chan; J P Atkinson
Journal:  J Immunol       Date:  1985-03       Impact factor: 5.422

8.  The novel complement inhibitor human CUB and Sushi multiple domains 1 (CSMD1) protein promotes factor I-mediated degradation of C4b and C3b and inhibits the membrane attack complex assembly.

Authors:  Astrid Escudero-Esparza; Nikolina Kalchishkova; Emila Kurbasic; Wen G Jiang; Anna M Blom
Journal:  FASEB J       Date:  2013-08-20       Impact factor: 5.191

Review 9.  Toward a structure-based comprehension of the lectin pathway of complement.

Authors:  Troels R Kjaer; Steffen Thiel; Gregers R Andersen
Journal:  Mol Immunol       Date:  2013-08-01       Impact factor: 4.407

10.  The human c1q globular domain: structure and recognition of non-immune self ligands.

Authors:  Christine Gaboriaud; Philippe Frachet; Nicole M Thielens; Gérard J Arlaud
Journal:  Front Immunol       Date:  2012-01-06       Impact factor: 7.561
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