Literature DB >> 33847930

Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay.

Per H Nilsson1,2, Kristin Pettersen3, Martin Oppermann4, Espen W Skjeflo3,5, Hilde Fure3, Dorte Christiansen3, Tom Eirik Mollnes6,5,7.   

Abstract

Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.

Entities:  

Keywords:  Anaphylatoxin; C3a; Complement; Complement activation; ELISA; Porcine

Year:  2021        PMID： 33847930     DOI： 10.1007/978-1-0716-1016-9_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  Effect of time, temperature and anticoagulants on in vitro complement activation: consequences for collection and preservation of samples to be examined for complement activation.

Authors:  T E Mollnes; P Garred; G Bergseth
Journal:  Clin Exp Immunol       Date:  1988-09       Impact factor: 4.330
  1 in total
  3 in total

1.  Venous Air Embolism Activates Complement C3 Without Corresponding C5 Activation and Trigger Thromboinflammation in Pigs.

Authors:  Benjamin S Storm; Judith K Ludviksen; Dorte Christiansen; Hilde Fure; Kristin Pettersen; Anne Landsem; Bent Aksel Nilsen; Knut Dybwik; Tonje Braaten; Erik W Nielsen; Tom E Mollnes
Journal:  Front Immunol       Date:  2022-03-15       Impact factor: 7.561

2.  A Novel Porcine Model of Ischemia-Reperfusion Injury After Cross-Clamping the Thoracic Aorta Revealed Substantial Cardiopulmonary, Thromboinflammatory and Biochemical Changes Without Effect of C1-Inhibitor Treatment.

Authors:  Erik Waage Nielsen; Yoav Miller; Ole-Lars Brekke; Joost Grond; Anh Hoang Duong; Hilde Fure; Judith Krey Ludviksen; Kristin Pettersen; Leon Reubsaet; Rigmor Solberg; Harald Thidemann Johansen; Tom Eirik Mollnes
Journal:  Front Immunol       Date:  2022-04-01       Impact factor: 7.561

3.  Complement Is Activated During Normothermic Machine Perfusion of Porcine and Human Discarded Kidneys.

Authors:  Neeltina M Jager; Leonie H Venema; Asel S Arykbaeva; Anita H Meter-Arkema; Petra J Ottens; Cees van Kooten; Tom E Mollnes; Ian P J Alwayn; Henri G D Leuvenink; Soeren E Pischke
Journal:  Front Immunol       Date:  2022-07-13       Impact factor: 8.786
  3 in total

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