A-Reum Lee1, Hyunsu Kim1, Kyung-Yoon Jeon1, Eun-Ji Ko1, Ahran Kim2, Nameun Kim3, HyeongJin Roh3, Yoonhang Lee3, Jiyeon Park3, Do-Hyung Kim3, Yung Hyun Choi4, Suhkmann Kim5, Heui-Soo Kim6, Mee Sun Ock1, Hee-Jae Cha7. 1. Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, Republic of Korea. 2. Pathology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea. 3. Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, 34, Amnam-dong, Seo-gu, Busan, 602-703, Republic of Korea. 4. Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan, Republic of Korea. 5. Department of Chemistry, College of Natural Sciences, Pusan National University, Busan, Republic of Korea. 6. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, Republic of Korea. 7. Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, Republic of Korea. hcha@kosin.ac.kr.
Abstract
BACKGROUND: Olive flounder (Paralichthys olivaceus) is one of the major cultured fish species in Asia including Korea. However, the mass mortality of olive flounder caused by various pathogens leads to huge economic loss. The pathogens that lead to fish mortality include parasites, bacteria, and viruses that can cause various kinds of diseases. OBJECTIVE: The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive flounder after artificial infection. We hypothesized that proteomics levels in gills and spleen may be differentially expressed depending on infectious agents. METHODS: To investigate the expression pattern of proteins in gills and spleens, olive flounders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive flounder. We used 2-DE analysis with LC-MS/MS to investigate proteome changes in infected olive flounders. RESULTS: The results of the LC-MS/MS analyses showed different protein expression profiles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profiles of S. parauberis and VHSV infection groups had many similarities, but the profile of the M. avidus infection group was greatly different in the gill and spleen. CONCLUSION: Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fish.
BACKGROUND: Olive flounder (Paralichthys olivaceus) is one of the major cultured fish species in Asia including Korea. However, the mass mortality of olive flounder caused by various pathogens leads to huge economic loss. The pathogens that lead to fish mortality include parasites, bacteria, and viruses that can cause various kinds of diseases. OBJECTIVE: The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive flounder after artificial infection. We hypothesized that proteomics levels in gills and spleen may be differentially expressed depending on infectious agents. METHODS: To investigate the expression pattern of proteins in gills and spleens, olive flounders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive flounder. We used 2-DE analysis with LC-MS/MS to investigate proteome changes in infected olive flounders. RESULTS: The results of the LC-MS/MS analyses showed different protein expression profiles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profiles of S. parauberis and VHSV infection groups had many similarities, but the profile of the M. avidus infection group was greatly different in the gill and spleen. CONCLUSION: Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fish.
Authors: Mi Young Cho; Unn Hwa Lee; Chang Hoon Moon; Jong Deuk Bang; Bo Young Jee; Seung Ju Cha; Jin Woo Kim; Myoung Ae Park; Jeong Wan Do; Jeong Woo Park Journal: Dis Aquat Organ Date: 2012-11-08 Impact factor: 1.802