Rouxiella aceris sp. nov., isolated from tree sap and the emended description of the genus Rouxiella.

Polyphasic taxonomic studies were performed for the seven strains, which were isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea. Cells of all the isolates were Gram-reaction-negative, facultatively anaerobic, short rods and contained the major isoprenoid quinone of Q-8, the predominant fatty acids of C16:0 and C17:0 cyclo and the major polar lipids including phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. The G + C contents of the genomic DNAs were 50.6-51.3%.The 16S rRNA gene-based phylogeny exhibited that the seven isolates formed two distinct sublines within the family Yersiniaceae. In the 92 core gene analysis, strain SAP-1T formed a subline at the base of radiation of the genus Rouxiella and its assignment to the genus Rouxiella was supported by high amino acid identity values (82.0-83.4%), albeit with sharing low 16S rRNA gene identities (96.0-96.9%). The average nucleotide identity and digital DNA-DNA hybridisation values together with phenotypic differences showed that strains SAP-1T, SAP-7, SAP-8 and SAP-13 belonged to a new species of the genus Rouxiella, while strains SAP-2, SAP-3 and SAP-27 were strains of Rouxiella silvae. On the basis of data obtained here, Rouxiella aceris sp. nov. (type strain, SAP-1T = KCTC 72599T = CCM 9078T) is proposed, with the emended description of the genus Rouxiella.