Di Li1, Jinwei Ren2, Qian Du2, Peng Liu1, Yong Li2. 1. Department of Clinical Nutrition, Peking University People's Hospital Beijing 100044, PR China. 2. Department of Nutrition and Food Hygiene, School of Public Health, Peking University Beijing 100191, PR China.
Abstract
OBJECTIVE: To explore the anti-hypoxic effects of oat oligopeptides (OOPs) in mice. METHODS: We randomly divided mice into six groups, including a vehicle control group, a whey protein group (0.50 g/kg), and four OOPs-treated groups (0.25, 0.50, 1.00, and 2.00 g/kg). The test substances were administered by gavage once a day for 30 days. The normobaric hypoxia, sodium nitrite toxicosis, and acute cerebral ischemia survival times were recorded. Also, the MDA content, the lactate levels, the LDH activity, and the mRNA levels of HIF-1α and VEGF in the brains were measured. We performed a whole blood cell analysis using a blood analyzer. RESULTS: The OOPs significantly extended the survival times of normobaric hypoxia, sodium nitrite toxicosis, and acute cerebral ischemia. Notably, the OOPs enhanced the RBC, Hb, and Hct levels, decreased the malonaldehyde (MDA) and lactate content in the brain, enhanced the brain lactate dehydrogenase (LDH) activity, and increased the hypoxia-inducible factor 1alpha (HIF1α) mRNA and the vascular endothelial growth factor (VEGF) mRNA expression levels. CONCLUSION: OOPs have anti-hypoxic effects, and the mechanism may involve improving the blood's oxygen carrying capacity and oxygen utilization rate minimizing the lipid peroxidation lesions, increasing the brain's ability to buffer against lactic acidosis in mice, and promoting angiogenesis and regulating the hypoxic response. AJTR
OBJECTIVE: To explore the anti-hypoxic effects of oat oligopeptides (OOPs) in mice. METHODS: We randomly divided mice into six groups, including a vehicle control group, a whey protein group (0.50 g/kg), and four OOPs-treated groups (0.25, 0.50, 1.00, and 2.00 g/kg). The test substances were administered by gavage once a day for 30 days. The normobaric hypoxia, sodium nitritetoxicosis, and acute cerebral ischemia survival times were recorded. Also, the MDA content, the lactate levels, the LDH activity, and the mRNA levels of HIF-1α and VEGF in the brains were measured. We performed a whole blood cell analysis using a blood analyzer. RESULTS: The OOPs significantly extended the survival times of normobaric hypoxia, sodium nitritetoxicosis, and acute cerebral ischemia. Notably, the OOPs enhanced the RBC, Hb, and Hct levels, decreased the malonaldehyde (MDA) and lactate content in the brain, enhanced the brain lactate dehydrogenase (LDH) activity, and increased the hypoxia-inducible factor 1alpha (HIF1α) mRNA and the vascular endothelial growth factor (VEGF) mRNA expression levels. CONCLUSION:OOPs have anti-hypoxic effects, and the mechanism may involve improving the blood's oxygen carrying capacity and oxygen utilization rate minimizing the lipid peroxidation lesions, increasing the brain's ability to buffer against lactic acidosis in mice, and promoting angiogenesis and regulating the hypoxic response. AJTR
Authors: Juan Wang; Lan Zhou Li; Yan Ge Liu; Li Rong Teng; Jia Hui Lu; Jing Xie; Wen Ji Hu; Yan Liu; Yang Liu; Di Wang; Le Sheng Teng Journal: Mol Med Rep Date: 2015-12-30 Impact factor: 2.952
Authors: Xuguang Zhang; Susan C McGeoch; Ian L Megson; Sandra M MacRury; Alexandra M Johnstone; Prakash Abraham; Donald W M Pearson; Baukje de Roos; Grietje Holtrop; Niamh O'Kennedy; Gerald E Lobley Journal: Mol Nutr Food Res Date: 2014-03-07 Impact factor: 5.914