Zibo Zhao1,2, Daowu Hu2, Muhammad Tehseen Azhar3, Hongge Li2, Chenhui Ma2, Shoupu He2, Xiaoyang Wang2, Gaofei Sun4, Tahir Mahmood2, Washu Dev2, Xiongming Du5,6. 1. School of Agriculture Sciences, Zhengzhou University, Zhengzhou, 450000, Henan, China. 2. Institute of Cotton Research of Chinese Academy of Agricultural Science/State Key Laboratory of Cotton Biology, Anyang, 455000, Henan, China. 3. Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 608000, Pakistan. 4. Anyang Institute of Technology, Anyang, 455000, China. 5. School of Agriculture Sciences, Zhengzhou University, Zhengzhou, 450000, Henan, China. dujeffrey8848@hotmail.com. 6. Institute of Cotton Research of Chinese Academy of Agricultural Science/State Key Laboratory of Cotton Biology, Anyang, 455000, Henan, China. dujeffrey8848@hotmail.com.
Abstract
MAIN CONCLUSION: The significant number loci and candidate genes of root color in Gossypium arboreum are identified and provide a theoretical basis of root color for cotton. A stimulating phenomenon was observed on the 4th day of sowing in the root color of some G. arboreum accessions that turned red. To disclose the genetic mechanisms of root color formation via genome and transcript levels, we identified the significant number of SNPs and candidate genes that are related to root color through genome-wide association study (GWAS) and RNAseq analysis in G. arboreum. Initially, 215 no. of G. arboreum accessions was collected, and the colors of root on the 4th, 6th and 9th day of germination were recorded. The GWAS demonstrated that 225 significant SNPs and 47 candidate genes have been identified totally. The strongest signal SNP A04_91824 could greatly distinguish the root color with most "C" allele accessions have displayed white and "T" allele accessions displayed red. RNAseq was performed on accessions having the white and red root, and results revealed that 12 and 138 DEGs were detected on 2nd and 4th day, respectively. ACD6, UFGT, and LYM2 were the most related genes of root color, later, verified by qRT-PCR. The mature zone of red and the white roots was observed by the histological section method, and results shown that cells were more closely arranged in the white root, and both average cell length and cell width were longer in the red root. This study will be helpful to cotton breeders for utilization of several elite genes and related SNPs related to root color, in addition to find linkage with economically important traits of interests.
MAIN CONCLUSION: The significant number loci and candidate genes of root color in Gossypium arboreum are identified and provide a theoretical basis of root color for cotton. A stimulating phenomenon was observed on the 4th day of sowing in the root color of some G. arboreum accessions that turned red. To disclose the genetic mechanisms of root color formation via genome and transcript levels, we identified the significant number of SNPs and candidate genes that are related to root color through genome-wide association study (GWAS) and RNAseq analysis in G. arboreum. Initially, 215 no. of G. arboreum accessions was collected, and the colors of root on the 4th, 6th and 9th day of germination were recorded. The GWAS demonstrated that 225 significant SNPs and 47 candidate genes have been identified totally. The strongest signal SNP A04_91824 could greatly distinguish the root color with most "C" allele accessions have displayed white and "T" allele accessions displayed red. RNAseq was performed on accessions having the white and red root, and results revealed that 12 and 138 DEGs were detected on 2nd and 4th day, respectively. ACD6, UFGT, and LYM2 were the most related genes of root color, later, verified by qRT-PCR. The mature zone of red and the white roots was observed by the histological section method, and results shown that cells were more closely arranged in the white root, and both average cell length and cell width were longer in the red root. This study will be helpful to cotton breeders for utilization of several elite genes and related SNPs related to root color, in addition to find linkage with economically important traits of interests.
Entities:
Keywords:
Genome-wide association studies; Histological section; Root color; Transcriptome analysis
Authors: Philip J White; Timothy S George; Peter J Gregory; A Glyn Bengough; Paul D Hallett; Blair M McKenzie Journal: Ann Bot Date: 2013-07 Impact factor: 4.357