Literature DB >> 3383555

An accurate method for automated counting of silver grains in autoradiographs.

R L Reep1, W J Creegan.   

Abstract

A grain counting algorithm has been developed for use with an image analysis system interfaced with a microscope. The algorithm consists of a sequence of tests involving thresholding, prospective grain center detection, contrast, and three kinds of artifact detection. The function of each of these parameters is described. This algorithm is accurate to within 4% for those fields most frequently encountered, and to within 9% for all fields examined. Furthermore, it operates very quickly and at relatively low magnification, making it possible to scan large areas efficiently.

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Year:  1988        PMID： 3383555     DOI： 10.1016/0010-4809(88)90031-6

Source DB:  PubMed          Journal:  Comput Biomed Res        ISSN： 0010-4809


  4 in total

1.  Distribution of beta 1- and beta 2-adrenoceptors in mouse trachea and lung: a quantitative autoradiographic study.

Authors:  P J Henry; P J Rigby; R G Goldie
Journal:  Br J Pharmacol       Date:  1990-01       Impact factor: 8.739

2.  Quantitation of autoradiographic grains in different zones of articular cartilage with image analyzer.

Authors:  J J Parkkinen; K Paukkonen; E Pesonen; M J Lammi; S Markkanen; H J Helminen; M Tammi
Journal:  Histochemistry       Date:  1990

3.  Flat bed scanner in the quantitative assay of 35SO4-incorporation by X-ray film autoradiography of intervertebral disc sections.

Authors:  K Puustjärvi; M J Lammi; I Kiviranta; H J Helminen; M Tammi
Journal:  Histochemistry       Date:  1993-01

4.  Relationship between endothelin-1 binding site densities and constrictor activities in human and animal airway smooth muscle.

Authors:  P J Henry; P J Rigby; G J Self; J M Preuss; R G Goldie
Journal:  Br J Pharmacol       Date:  1990-08       Impact factor: 8.739
  4 in total

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