Gilberto Mendes Menderico Junior1, Thérèse Rachell Theodoro2, Fatima Solange Pasini3, Marina de Menezes Ishikawa4, Nayara Stephânie Sousa Santos5, Evandro Sobroza de Mello6, Maria Aparecida da Silva Pinhal2, Raquel Ajub Moyses7, Marco Aurelio Vamondes Kulcsar8, Rogério Aparecido Dedivitis9, Claudio Roberto Cernea9, Luiz Paulo Kowalski9, Leandro Luongo de Matos10. 1. Head and Neck Surgery Department, Anesthesiology, Surgical Sciences and Perioperative Medicine, University of Sao Paulo Medical School, Sao Paulo, Brazil. 2. Biochemistry Department, Faculdade de Medicina do ABC, Santo André, Brazil. 3. Centro de Investigação Translacional em Oncologia (CTO), Instituto do Cancer do Estado de Sao Paulo (ICESP), Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo (HCFMUSP), Sao Paulo, Brazil. 4. Nursery School, Faculdade Israelita de Ciências da Saúde Albert Einstein, Sao Paulo, Brazil. 5. University of Sao Paulo Medical School, Sao Paulo, Brazil. 6. Pathology Department, Instituto do Câncer do Estado de São Paulo (ICESP), Laboratório de Investigação Médica 14 (LIM14), University of Sao Paulo Medical School, Sao Paulo, Brazil. 7. Head and Neck Surgery Department, Laboratório de Investigação Médica 28 (LIM28), University of Sao Paulo Medical School, Sao Paulo, Brazil. 8. Head and Neck Surgery Department, Instituto do Câncer do Estado de São Paulo (ICESP), University of Sao Paulo Medical School, Sao Paulo, Brazil. 9. Head and Neck Surgery Department, University of Sao Paulo Medical School, Sao Paulo, Brazil. 10. Head and Neck Surgery Department, Instituto do Câncer do Estado de São Paulo (ICESP), Laboratório de Investigação Médica 28 (LIM28), University of Sao Paulo Medical School, Sao Paulo, Brazil.
Abstract
BACKGROUND: We evaluated microRNAs and extracellular matrix component profiles in squamous cell carcinoma of the oral cavity (OSCC) in comparison to healthy mucosa. METHODS: Retrospective study investigating 64 microRNAs related to oncogenic process and to constituents of the extracellular matrix. We also performed immunohistochemical assays for molecules involved in the same biological processes. RESULTS: High expression of miR-21-5p (p < 0.001) and miR-106-5p (p < 0.001) and low expression of miR-320a (p = 0.001) and miR-222-3p (p = 0.001) were predictors of malignancy. Individually, miR-21-5p exhibited the best statistical performance (area under the curve = 0.972; 95% confidence interval: 0.911-1.000) in the differentiation between tumor tissue and healthy mucosa. Moreover, tumor sample showed increased expression of MMP-2, MMP-9, α-laminin, and β-laminin in tumor-related fibroblasts and lower continuity of type IV collagen in the basement membrane. CONCLUSION: The present study demonstrates the biological effects of microRNAs on the carcinogenesis of OSCC as well as the intense modification of the tumor microenvironment.
BACKGROUND: We evaluated microRNAs and extracellular matrix component profiles in squamous cell carcinoma of the oral cavity (OSCC) in comparison to healthy mucosa. METHODS: Retrospective study investigating 64 microRNAs related to oncogenic process and to constituents of the extracellular matrix. We also performed immunohistochemical assays for molecules involved in the same biological processes. RESULTS: High expression of miR-21-5p (p < 0.001) and miR-106-5p (p < 0.001) and low expression of miR-320a (p = 0.001) and miR-222-3p (p = 0.001) were predictors of malignancy. Individually, miR-21-5p exhibited the best statistical performance (area under the curve = 0.972; 95% confidence interval: 0.911-1.000) in the differentiation between tumor tissue and healthy mucosa. Moreover, tumor sample showed increased expression of MMP-2, MMP-9, α-laminin, and β-laminin in tumor-related fibroblasts and lower continuity of type IV collagen in the basement membrane. CONCLUSION: The present study demonstrates the biological effects of microRNAs on the carcinogenesis of OSCC as well as the intense modification of the tumor microenvironment.
Authors: Arsinoe C Thomaidou; Panagiota Batsaki; Maria Adamaki; Maria Goulielmaki; Constantin N Baxevanis; Vassilis Zoumpourlis; Sotirios P Fortis Journal: Int J Mol Sci Date: 2022-07-26 Impact factor: 6.208