| Literature DB >> 33827654 |
Helena García-Castro1, Nathan J Kenny1, Marta Iglesias2,3, Patricia Álvarez-Campos1,4, Vincent Mason1, Anamaria Elek2,3, Anna Schönauer1, Victoria A Sleight5, Jakke Neiro6, Aziz Aboobaker6, Jon Permanyer2,3, Manuel Irimia2,3,7, Arnau Sebé-Pedrós2,3, Jordi Solana8.
Abstract
Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.Entities:
Keywords: Combinatorial indexing; Dissociation; Fixation; Planarian; RNA-seq; SPLiT-seq; Single-cell transcriptomics
Mesh：
Year: 2021 PMID： 33827654 PMCID： PMC8028764 DOI： 10.1186/s13059-021-02302-5
Source DB: PubMed Journal: Genome Biol ISSN： 1474-7596 Impact factor: 17.906