Literature DB >> 3382677

Purification, characterization and kinetic properties of the rabbit gastric lipase.

H Moreau1, Y Gargouri, D Lecat, J L Junien, R Verger.   

Abstract

Rabbit gastric lipase was purified from an acetonic powder of rabbit stomach fundus. 25 mg of pure rabbit gastric lipase (glycerol ester hydrolase, EC 3.1.1.3) was obtained from 30 rabbit stomachs after ammonium sulfate fractionation, Sephadex G-100 gel filtration and cation exchange (mono S column) using a fast protein liquid chromatography (FPLC) system. The pure enzyme obtained was resistant to acidic pH conditions, and had specific activities of 1200, 850 and 280 U/mg, using, respectively, short- (tributyroylglycerol (TC4)), medium- (trioctanoyl- to tridecanoylglycerol (TC8-TC10)) and long-chain (soybean oil) triacylglycerols. The amino-acid composition was determined, and the first 30 N-terminal amino-acid residues were sequenced. Interfacial denaturation and catalytic properties on triacylglycerol emulsions were studied. Rabbit gastric lipase turned out to be structurally and kinetically very similar to human gastric lipase.

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Year:  1988        PMID： 3382677     DOI： 10.1016/0005-2760(88)90036-7

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

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Journal:  J Lipid Res       Date:  2015-03-07       Impact factor: 5.922

2.  INFOGEST static in vitro simulation of gastrointestinal food digestion.

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Journal:  Nat Protoc       Date:  2019-03-18       Impact factor: 13.491

Review 3.  Lipids in the Stomach - Implications for the Evaluation of Food Effects on Oral Drug Absorption.

Authors:  Mirko Koziolek; Frédéric Carrière; Christopher J H Porter
Journal:  Pharm Res       Date:  2018-02-08       Impact factor: 4.200

4.  Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum.

Authors:  Jörg M Steiner; David A Williams
Journal:  Can J Vet Res       Date:  2004-07       Impact factor: 1.310

5.  Purification and characterization of lamb pregastric lipase.

Authors:  T M D'Souza; P Oriel
Journal:  Appl Biochem Biotechnol       Date:  1992-09       Impact factor: 2.926

6.  Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs.

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Journal:  Nutrients       Date:  2021-01-28       Impact factor: 5.717

7.  In Silico Evaluation of Enzymatic Tunnels in the Biotransformation of α-Tocopherol Esters.

Authors:  Tamara Stela Mendonça Azevedo; Lavínia Kelly Barros Silva; Álvaro Silva Lima; Matheus Mendonça Pereira; Elton Franceschi; Cleide Mara Faria Soares
Journal:  Front Bioeng Biotechnol       Date:  2022-01-21
  7 in total

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