George Vartholomatos1, Haralambos Harissis2, Maria Andreou3, Vissaria Tatsi2, Lamprini Pappa4, Sevasti Kamina5, Anna Batistatou5, Georgios S Markopoulos6, George A Alexiou7. 1. Haematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, Ioannina, Greece; Neurosurgical Institute, University of Ioannina School of Medicine, Ioannina, Greece. 2. Breast Unit, University Hospital of Ioannina, Greece. 3. Haematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, Ioannina, Greece. 4. Department of Cytology, University Hospital of Ioannina, Ioannina, Greece. 5. Department of Pathology, University Hospital of Ioannina, Ioannina, Greece. 6. Neurosurgical Institute, University of Ioannina School of Medicine, Ioannina, Greece. 7. Neurosurgical Institute, University of Ioannina School of Medicine, Ioannina, Greece; Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece. Electronic address: galexiou@uoi.gr.
Abstract
BACKGROUND: Positive margins are the most important factor for recurrence of the disease after breast-conserving surgery. Several methods have been developed throughout the years to evaluate the margin status during surgery in an attempt to assist the surgeon in excising the whole tumor at once, a goal that has not yet been accomplished. PATIENTS AND METHODS: In our study, we compared intraoperative flow cytometry (iFC) with cytology and pathology in order to evaluate 606 samples of margins and tumors corresponding to 99 patients with invasive ductal carcinoma of no special type and invasive lobular carcinoma obtained from breast-conserving surgeries. RESULTS: Using the pathology as the gold standard, flow cytometry had 93.3% sensitivity, 92.4% specificity, and 92.5% accuracy. Cytology had 82.3% sensitivity, 94.6% specificity, and 94.2% accuracy. CONCLUSIONS: Our data support the suggestion that iFC is a novel, reliable technique that allows rapid evaluation of the excision margins of lumpectomies, thus improving the precision of breast-conserving surgery. Among the advantages of iFC are that it does not rely on the expertise of a pathologist or cytologist, it is low cost, and it has no additional psychological effect on patients, because no re-operation is needed.
BACKGROUND: Positive margins are the most important factor for recurrence of the disease after breast-conserving surgery. Several methods have been developed throughout the years to evaluate the margin status during surgery in an attempt to assist the surgeon in excising the whole tumor at once, a goal that has not yet been accomplished. PATIENTS AND METHODS: In our study, we compared intraoperative flow cytometry (iFC) with cytology and pathology in order to evaluate 606 samples of margins and tumors corresponding to 99 patients with invasive ductal carcinoma of no special type and invasive lobular carcinoma obtained from breast-conserving surgeries. RESULTS: Using the pathology as the gold standard, flow cytometry had 93.3% sensitivity, 92.4% specificity, and 92.5% accuracy. Cytology had 82.3% sensitivity, 94.6% specificity, and 94.2% accuracy. CONCLUSIONS: Our data support the suggestion that iFC is a novel, reliable technique that allows rapid evaluation of the excision margins of lumpectomies, thus improving the precision of breast-conserving surgery. Among the advantages of iFC are that it does not rely on the expertise of a pathologist or cytologist, it is low cost, and it has no additional psychological effect on patients, because no re-operation is needed.
Authors: Georgios S Markopoulos; Anna Goussia; Christina D Bali; Thomas Messinis; George Α Alexiou; George Vartholomatos Journal: Ann Surg Oncol Date: 2022-03-18 Impact factor: 5.344
Authors: George Vartholomatos; Haralambos Harissis; Georgios S Markopoulos; George A Alexiou Journal: Ann Surg Oncol Date: 2021-09-14 Impact factor: 5.344