Literature DB >> 3382014

Scintillation counting of 14C-labeled soluble and insoluble compounds in plant tissue.

D Sun1, L E Wimmers, R Turgeon.   

Abstract

A method is described for the liquid scintillation counting of 14C in plant tissues. Samples are fixed, in the scintillation vial, in a solution of ethanol and acetic acid (3:1) and decolorized with commercial bleach before the addition of scintillation liquid. The method was compared to other techniques of tissue oxidation or digestion and found to be equally effective at least with thin tissue samples. The technique is simple, rapid, and inexpensive and does not result in loss of 14C.

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Year:  1988        PMID: 3382014     DOI: 10.1016/0003-2697(88)90307-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  7 in total

1.  Sugar synthesis and phloem loading in Coleus blumei leaves.

Authors:  R Turgeon; E Gowan
Journal:  Planta       Date:  1992-06       Impact factor: 4.116

2.  Quantitative Analysis of Photosynthate Unloading in Developing Seeds of Phaseolus vulgaris L. : I. The Use of Steady-State Labeling.

Authors:  E C Ellis; R Turgeon; R M Spanswick
Journal:  Plant Physiol       Date:  1992-06       Impact factor: 8.340

3.  Phloem Loading in Coleus blumei in the Absence of Carrier-Mediated Uptake of Export Sugar from the Apoplast.

Authors:  R Turgeon; E Gowan
Journal:  Plant Physiol       Date:  1990-11       Impact factor: 8.340

4.  Phloem loading. A reevaluation of the relationship between plasmodesmatal frequencies and loading strategies.

Authors:  Robert Turgeon; Richard Medville
Journal:  Plant Physiol       Date:  2004-10-29       Impact factor: 8.340

5.  Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in Tomato Leaves Induced by Systemin and Jasmonic Acid.

Authors:  S. H. Doares; J. Narvaez-Vasquez; A. Conconi; C. A. Ryan
Journal:  Plant Physiol       Date:  1995-08       Impact factor: 8.340

6.  Photoassimilate-transport characteristics of nonchlorophyllous and green tissue in variegated leaves of Coleus blumei Benth.

Authors:  L A Weisberg; L E Wimmers; R Turgeon
Journal:  Planta       Date:  1988-07       Impact factor: 4.116

7.  Transfer cells and solute uptake in minor veins of Pisum sativum leaves.

Authors:  L E Wimmers; R Turgeon
Journal:  Planta       Date:  1991-12       Impact factor: 4.116

  7 in total

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