Literature DB >> 3382004

Extraction and measurement of myocardial nucleotides, nucleosides, and purine bases by high-performance liquid chromatography.

D F Hammer1, D V Unverferth, R E Kelley, P A Harvan, R A Altschuld.   

Abstract

Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid chromatography. To achieve the sensitivity necessary for analyzing small (1-3 mg wet wt) tissue samples, a small-bore, 2.1-mm-internal-diameter, C18, 5-micron reverse-phase column and a flow rate of 0.2 ml/min were used. All of the myocardial nucleotides and AMP degradation products were resolved in a total separation time of 27 min with 30 mM KH2PO4, 7.5 mM tetrabutylammonium phosphate buffers, and binary pH and acetonitrile gradients.

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Year:  1988        PMID： 3382004     DOI： 10.1016/0003-2697(88)90288-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Proceedings of the British Pharmacological Society Meeting. Sheffield, 18-20th April 1990.

Authors: 
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3.  Human myocardial ATP content and in vivo contractile function.

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Authors:  Dhaval P Bhatt; Heidi M Houdek; John A Watt; Thad A Rosenberger
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7.  Phosphorylation of adenosine in renal brush-border membrane vesicles by an exchange reaction catalysed by adenosine kinase.

Authors:  J Sayós; C Solsona; J Mallol; C Lluis; R Franco
Journal:  Biochem J       Date:  1994-02-01       Impact factor: 3.857
  7 in total

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