María Gabriela Mancilla-Montelongo1, Gloria Sarahí Castañeda-Ramírez2, Elodie Gaudin-Barbier3, María Librada Canul-Velasco2, José Israel Chan-Pérez2, Álvaro De la Cruz-Cortazar2, Celine Mathieu4,5, Isabelle Fourquaux6, Carlos Alfredo Sandoval-Castro2, Hervé Hoste3, Javier Ventura-Cordero7, Pedro Geraldo González-Pech2, Juan Felipe de Jesús Torres-Acosta8. 1. CONACYT-Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, CP 97100, Mérida, Yucatán, Mexico. 2. Facultad de Medicina Veterinaria Y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, CP 97100, Mérida, Yucatán, Mexico. 3. INRAE, UMR 1225 IHAP, ENVT, 23 Chemin des Capelles, 31076, Toulouse, France. 4. Laboratoire de Chimie Agro Industrielle, LCA, Université de Toulouse, INP, INRAE, 31030, Toulouse, France. 5. Centre d'Applications et de Traitement des Agro-Ressources (CATAR), INP, 31030, Toulouse, France. 6. Centre de Microscopie Appliquée à la Biologie (CMEAB), Medical Faculty, University Paul Sabatier, 31000, Toulouse, France. 7. School of Biological Sciences, Queen's University Belfast, Chlorine Gardens, Belfast, BT9 5BL, UK. 8. Facultad de Medicina Veterinaria Y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, CP 97100, Mérida, Yucatán, Mexico. tacosta@correo.uady.mx.
Abstract
PURPOSE: Some agroindustrial by-products could be used as nutraceutical materials for small ruminants helping with their nutrition while controlling their gastrointestinal nematodes. This study evaluated the potential in vitro nutraceutical value of pod husks and leaves of three varieties of Theobroma cacao using two Haemonchus contortus isolates with different polyphenol susceptibility. METHODS: Leaves and husks from three T. cacao varieties (AZT, CAL and CEY) were evaluated for their bromatological composition, in vitro dry matter digestibility and polyphenol content. Acetone:water extracts (70:30) of each plant variety were evaluated using the egg hatch and larval exsheathment inhibition tests, using two isolates (FESC and PARAISO) of H. contortus. Effective concentrations 50% (EC50) were determined for both tests. The role of polyphenols was confirmed using polyvinylpolypyrrolidone. L3 exposed to CAL leaf extract were submitted to transmission electron microscopy. RESULTS: Both plant materials showed a good nutritional value to complement protein-rich diets for small ruminants. Extracts inhibited exsheathment of H. contortus L3 more effectively than the egg hatching, and the leaf extracts were more active than husk extracts in the L3 exsheathment inhibition. The FESC isolate was more sensitive to extracts. Polyphenols blocked exsheathment inhibition of leaf extracts. Structural damage was observed in the sheath and muscles of L3 exposed to CAL leaf extracts. CONCLUSION: The two T. cacao materials tested showed their potential to be used as ruminant feeds. Extracts affected H. contortus by blocking L3 exsheathment, particularly with the leaf extracts. The in vivo nutraceutical value should be confirmed in small ruminants.
PURPOSE: Some agroindustrial by-products could be used as nutraceutical materials for small ruminants helping with their nutrition while controlling their gastrointestinal nematodes. This study evaluated the potential in vitro nutraceutical value of pod husks and leaves of three varieties of Theobroma cacao using two Haemonchus contortus isolates with different polyphenol susceptibility. METHODS: Leaves and husks from three T. cacao varieties (AZT, CAL and CEY) were evaluated for their bromatological composition, in vitro dry matter digestibility and polyphenol content. Acetone:water extracts (70:30) of each plant variety were evaluated using the egg hatch and larval exsheathment inhibition tests, using two isolates (FESC and PARAISO) of H. contortus. Effective concentrations 50% (EC50) were determined for both tests. The role of polyphenols was confirmed using polyvinylpolypyrrolidone. L3 exposed to CAL leaf extract were submitted to transmission electron microscopy. RESULTS: Both plant materials showed a good nutritional value to complement protein-rich diets for small ruminants. Extracts inhibited exsheathment of H. contortus L3 more effectively than the egg hatching, and the leaf extracts were more active than husk extracts in the L3 exsheathment inhibition. The FESC isolate was more sensitive to extracts. Polyphenols blocked exsheathment inhibition of leaf extracts. Structural damage was observed in the sheath and muscles of L3 exposed to CAL leaf extracts. CONCLUSION: The two T. cacao materials tested showed their potential to be used as ruminant feeds. Extracts affected H. contortus by blocking L3 exsheathment, particularly with the leaf extracts. The in vivo nutraceutical value should be confirmed in small ruminants.
Authors: H Hoste; J F J Torres-Acosta; J Quijada; I Chan-Perez; M M Dakheel; D S Kommuru; I Mueller-Harvey; T H Terrill Journal: Adv Parasitol Date: 2016-05-11 Impact factor: 3.870
Authors: G S Castañeda-Ramírez; M Rodríguez-Labastida; G I Ortiz-Ocampo; P G González-Pech; J Ventura-Cordero; R Borges-Argáez; J F J Torres-Acosta; C A Sandoval-Castro; C Mathieu Journal: Parasitol Res Date: 2018-10-16 Impact factor: 2.289
Authors: F A Méndez-Ortiz; C A Sandoval-Castro; J Ventura-Cordero; L A Sarmiento-Franco; R H Santos-Ricalde; J F J Torres-Acosta Journal: Trop Anim Health Prod Date: 2019-03-02 Impact factor: 1.559
Authors: J J Vargas-Magaña; J F J Torres-Acosta; A J Aguilar-Caballero; C A Sandoval-Castro; H Hoste; J A Chan-Pérez Journal: Vet Parasitol Date: 2014-12-15 Impact factor: 2.738
Authors: H Hoste; J F J Torres-Acosta; C A Sandoval-Castro; I Mueller-Harvey; S Sotiraki; H Louvandini; S M Thamsborg; T H Terrill Journal: Vet Parasitol Date: 2015-07-02 Impact factor: 2.738
Authors: G F Castillo-Mitre; A Olmedo-Juárez; R Rojo-Rubio; M González-Cortázar; P Mendoza-de Gives; E E Hernández-Beteta; D E Reyes-Guerrero; M E López-Arellano; J F Vázquez-Armijo; G Ramírez-Vargas; A Zamilpa Journal: J Ethnopharmacol Date: 2017-04-14 Impact factor: 4.360
Authors: Hervé Hoste; Griselda Meza-OCampos; Sarah Marchand; Smaragda Sotiraki; Katerina Sarasti; Berit M Blomstrand; Andrew R Williams; Stig M Thamsborg; Spiridoula Athanasiadou; Heidi L Enemark; Juan Felipe Torres Acosta; Gabriella Mancilla-Montelongo; Carlos Sandoval Castro; Livio M Costa-Junior; Helder Louvandini; Dauana Mesquita Sousa; Juha-Pekka Salminen; Maarit Karonen; Marika Engstrom; Johannes Charlier; Vincent Niderkorn; Eric R Morgan Journal: Parasite Date: 2022-02-28 Impact factor: 3.000