Ekaterina Borvinskaya1, Albina Kochneva2, Daria Bedulina3, Irina Sukhovskaya2, Lev Smirnov2, Irina Babkina4. 1. Institute of Biology At Irkutsk State University, 3 Lenin St, 664025, Irkutsk, Russia. borvinska@gmail.com. 2. Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya St, 185910, Petrozavodsk, Russia. 3. Institute of Biology At Irkutsk State University, 3 Lenin St, 664025, Irkutsk, Russia. 4. Department of Invertebrate Zoology, St Petersburg State University, 7/9A Universitetskaya St, 199034, St Petersburg, Russia.
Abstract
PURPOSE: Studies of proteins expressed in the morphological structures of the parasite are necessary for elucidating the biological functions of unknown proteins and understanding the molecular basis of parasitism. The research aim was to investigate the spatial distribution of major proteins in scolex, immature and gravid proglottids of Triaenophorus nodulosus and Triaenophorus crassus. METHODS: Protein extracts of worm body parts were analyzed using two-dimensional difference gel electrophoresis (DIGE) and mass spectrometry. RESULTS: Comparison of the protein repertoire of the adult worm and the encysted plerocercoid revealed differences between the worm body parts, life stages and parasite species. The content of proteins associated with the cytoskeleton and musculature (actin, myosin regulatory light chain, and tropomyosin 2) decreased with distance from the scolex. Mature proglottids were rich in transforming growth factor-beta-induced protein, propionyl-CoA carboxylase, glutamate dehydrogenase and beta-tubulin. Interspecific variation in T. nodulosus and T. crassus was found in the content of the myosin, paramyosin, the major vault protein and an uncharacterized secreted protein TRINITY_DN24645. Differential expression of TRINITY_DN24645, paramyosin and tropomyosin 2 was found between plerocercoids and adult worms. CONCLUSION: The present study provides the first characteristics of the spatial distribution of the major proteins of T. crassus and T. nodulosus. Comparison of the protein composition of plerocercoids and adult parasites indicates a significant similarity in the proteomic organization of Triaenophorus sp. in the second intermediate and final hosts. The gradual change in the morphological organization of tapeworms in the longitudinal direction coincided with the expression of some structural and metabolic proteins.
PURPOSE: Studies of proteins expressed in the morphological structures of the parasite are necessary for elucidating the biological functions of unknown proteins and understanding the molecular basis of parasitism. The research aim was to investigate the spatial distribution of major proteins in scolex, immature and gravid proglottids of Triaenophorus nodulosus and Triaenophorus crassus. METHODS: Protein extracts of worm body parts were analyzed using two-dimensional difference gel electrophoresis (DIGE) and mass spectrometry. RESULTS: Comparison of the protein repertoire of the adult worm and the encysted plerocercoid revealed differences between the worm body parts, life stages and parasite species. The content of proteins associated with the cytoskeleton and musculature (actin, myosin regulatory light chain, and tropomyosin 2) decreased with distance from the scolex. Mature proglottids were rich in transforming growth factor-beta-induced protein, propionyl-CoA carboxylase, glutamate dehydrogenase and beta-tubulin. Interspecific variation in T. nodulosus and T. crassus was found in the content of the myosin, paramyosin, the major vault protein and an uncharacterized secreted protein TRINITY_DN24645. Differential expression of TRINITY_DN24645, paramyosin and tropomyosin 2 was found between plerocercoids and adult worms. CONCLUSION: The present study provides the first characteristics of the spatial distribution of the major proteins of T. crassus and T. nodulosus. Comparison of the protein composition of plerocercoids and adult parasites indicates a significant similarity in the proteomic organization of Triaenophorus sp. in the second intermediate and final hosts. The gradual change in the morphological organization of tapeworms in the longitudinal direction coincided with the expression of some structural and metabolic proteins.
Authors: Isheng J Tsai; Magdalena Zarowiecki; Nancy Holroyd; Alejandro Garciarrubio; Alejandro Sánchez-Flores; Karen L Brooks; Alan Tracey; Raúl J Bobes; Gladis Fragoso; Edda Sciutto; Martin Aslett; Helen Beasley; Hayley M Bennett; Xuepeng Cai; Federico Camicia; Richard Clark; Marcela Cucher; Nishadi De Silva; Tim A Day; Peter Deplazes; Karel Estrada; Cecilia Fernández; Peter W H Holland; Junling Hou; Songnian Hu; Thomas Huckvale; Stacy S Hung; Laura Kamenetzky; Jacqueline A Keane; Ferenc Kiss; Uriel Koziol; Olivia Lambert; Kan Liu; Xuenong Luo; Yingfeng Luo; Natalia Macchiaroli; Sarah Nichol; Jordi Paps; John Parkinson; Natasha Pouchkina-Stantcheva; Nick Riddiford; Mara Rosenzvit; Gustavo Salinas; James D Wasmuth; Mostafa Zamanian; Yadong Zheng; Jianping Cai; Xavier Soberón; Peter D Olson; Juan P Laclette; Klaus Brehm; Matthew Berriman Journal: Nature Date: 2013-03-13 Impact factor: 49.962