Katarína Ondreičková1, Michaela Piliarová2, Lenka Klčová1, Alžbeta Žofajová1, Jozef Gubiš1, Miroslav Horník3, Marcela Gubišová1, Martina Hudcovicová1, Ján Kraic1,2. 1. Department of Applied Biology and Genetics, National Agricultural and Food Centre - Research Institute of Plant Production, Bratislavská cesta 122, 921 68, Piešťany, Slovak Republic. 2. Department of Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 917 01, Trnava, Slovak Republic. 3. Department of Ecochemistry and Radioecology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 917 01, Trnava, Slovak Republic.
Abstract
Current problems with sewage sludge (SS) disposal could be solved by application to agricultural land considering its fertilizer properties and ability to improve soil condition. However, SS may contain heavy metals as well as pathogenic microorganisms. In this study, molecular analysis of partial 18S rRNA gene was used to study the impact of SS application into the soil on the genetic diversity of fungal communities, especially arbuscular mycorrhizal fungi in the rhizosphere and roots of barley. These samples were collected on three dates from the control soil without SS and from the soil with the addition of SS at the concentrations of 5 and 15 t ha-1. Fungal alpha diversity in the rhizosphere of barley was affected by SS differently than in barley roots. In addition, principal component analysis and cluster analysis revealed that fungal communities were strongly influenced by the SS addition into the soil, sample type, and the sampling date. This approach was complemented by an evaluation of the basic parameters of barley production and the response of these parameters to the presence of SS in the soil. The plant height increased with increasing SS concentration and the thousand seed weight significantly increased at the concentration of 5 t ha-1 SS but significantly decreased in 15 t ha-1.
pan class="Chemical">Current problems with sewage sludge (pan class="Chemical">SS) disposal could be solved by application to agricultural land considering its fertilizer properties and ability to improve soil condition. However, SS may contain heavy metals as well as pathogenic microorganisms. In this study, molecular analysis of partial 18S rRNA gene was used to study the impact of SS application into the soil on the genetic diversity of fungal communities, especially arbuscular mycorrhizal fungi in the rhizosphere and roots of barley. These samples were collected on three dates from the control soil without SS and from the soil with the addition of SS at the concentrations of 5 and 15 t ha-1. Fungal alpha diversity in the rhizosphere of barley was affected by SS differently than in barley roots. In addition, principal component analysis and cluster analysis revealed that fungal communities were strongly influenced by the SS addition into the soil, sample type, and the sampling date. This approach was complemented by an evaluation of the basic parameters of barley production and the response of these parameters to the presence of SS in the soil. The plant height increased with increasing SS concentration and the thousand seed weight significantly increased at the concentration of 5 t ha-1 SS but significantly decreased in 15 t ha-1.
Authors: E Jacquot-Plumey; D van Tuinen; O Chatagnier; S Gianinazzi; V Gianinazzi-Pearson Journal: Environ Microbiol Date: 2001-08 Impact factor: 5.491
Authors: Jacqueline M Chaparro; Dayakar V Badri; Matthew G Bakker; Akifumi Sugiyama; Daniel K Manter; Jorge M Vivanco Journal: PLoS One Date: 2013-02-01 Impact factor: 3.240