Jin-Liang Li1, Zai-Qiu Wang2, Xiao-Li Sun3. 1. Department of Anus & Intestine Surgery, The First People's Hospital of Jining, Jining, Shandong, 272100, P.R. China. 2. Department of Anorectal Surgery, Yantai Yuhuangding Hospital, Yantai, 264000, P.R. China. 3. Department of Clinical Laboratory, Yantai Yuhuangding Hospital, Yantai, 264000, P.R. China.
Abstract
OBJECTIVE: This study was designed to explore the biological significance of myosin light chain 6B (MYL6B) in rectal adenocarcinoma. METHODS: Profiles on the Oncomine dataset, GEPIA website, and UALCAN-TCGA database were searched to assess the MYL6B expression level in rectal adenocarcinoma tissues and normal tissues. After MYL6B knockdown using siRNA strategy, cell counting kit-8 (CCK-8) and transwell assays were conducted to measure cell proliferation, migration and invasion, respectively. Flow cytometry analysis was conducted to assess cell apoptosis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot were performed to detect the expression level of mRNAs and proteins. RESULTS: The data showed that overexpression of MYL6B was observed in rectal adenocarcinoma tissues and correlated with a poor prognosis of patients. Functional in vitro experiments revealed that MYL6B knockdown could inhibit proliferation, migration, and invasion of rectal adenocarcinoma cells, while promote cell apoptosis. Moreover, western blot analysis suggested that increased expression of E-cadherin and decreased expression of N-cadherin and Vimentin were induced by si-MYL6B. CONCLUSION: In summary, this study elaborated on the promoting effect of MYL6B in rectal adenocarcinoma progression, thus providing novel insight for strategies of clinical diagnosis and drug application in the future clinical study.
OBJECTIVE: This study was designed to explore the biological significance of myosin light chain 6B (MYL6B) in rectal adenocarcinoma. METHODS: Profiles on the Oncomine dataset, GEPIA website, and UALCAN-TCGA database were searched to assess the MYL6B expression level in rectal adenocarcinoma tissues and normal tissues. After MYL6B knockdown using siRNA strategy, cell counting kit-8 (CCK-8) and transwell assays were conducted to measure cell proliferation, migration and invasion, respectively. Flow cytometry analysis was conducted to assess cell apoptosis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot were performed to detect the expression level of mRNAs and proteins. RESULTS: The data showed that overexpression of MYL6B was observed in rectal adenocarcinoma tissues and correlated with a poor prognosis of patients. Functional in vitro experiments revealed that MYL6B knockdown could inhibit proliferation, migration, and invasion of rectal adenocarcinoma cells, while promote cell apoptosis. Moreover, western blot analysis suggested that increased expression of E-cadherin and decreased expression of N-cadherin and Vimentin were induced by si-MYL6B. CONCLUSION: In summary, this study elaborated on the promoting effect of MYL6B in rectal adenocarcinoma progression, thus providing novel insight for strategies of clinical diagnosis and drug application in the future clinical study.
Authors: Zoltan Maliga; Magno Junqueira; Yusuke Toyoda; Andreas Ettinger; Felipe Mora-Bermúdez; Robin W Klemm; Andrej Vasilj; Elaine Guhr; Itziar Ibarlucea-Benitez; Ina Poser; Ezio Bonifacio; Wieland B Huttner; Andrej Shevchenko; Anthony A Hyman Journal: Nat Cell Biol Date: 2013-02-17 Impact factor: 28.824
Authors: Antti Arjonen; Riina Kaukonen; Elina Mattila; Pegah Rouhi; Gunilla Högnäs; Harri Sihto; Bryan W Miller; Jennifer P Morton; Elmar Bucher; Pekka Taimen; Reetta Virtakoivu; Yihai Cao; Owen J Sansom; Heikki Joensuu; Johanna Ivaska Journal: J Clin Invest Date: 2014-03 Impact factor: 14.808