Wei Hong1,2, Jing Yang3, Yumei Cheng4, Xiaolin Huang5, Fengqin Rao1,2, Ting Zhang1,2, Pixiang Wang6, Jian Liao5, Xiaolan Qi1,2, Zhizhong Guan1,2, Zhenhong Chen7, Guzhen Cui7. 1. Key Laboratory of Endemic and Ethnic Diseases, Guizhou Medical University, Ministry of Education, Guiyang 55004, Guizhou, China. 2. Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Guiyang 55004, Guizhou, China. 3. Guiyang Maternal and Child Health Hospital, Guiyang 550004, Guizhou, China. 4. Department of Critical Care Medicine, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China. 5. School/Hospital of Stomatology of Guizhou Medical University, Guiyang 550004, Guizhou, China. 6. Department of Biosystems Engineering, Auburn University, Auburn, Alabama 36849, United States. 7. School of Basic Medical Science, Guizhou Medical University, Guiyang, 550025, China Guiyang 550025, Guizhou, China.
Abstract
BACKGROUND: Clostridium difficile infection (CDI) is the leading cause of nosocomial diarrhea. Co-colonization of key bacterial taxa may prevent the transition from asymptomatic C. difficile colonization to CDI. However, little is known about the composition of key bacterial taxa in asymptomatic patients. METHODS: In the present study, the culture method was used to examine the composition of stool microbiota in two asymptomatic patients from Guizhou, China. RESULTS: A total of 111 strains were isolated and phylogenetic relationships were determined by 16S ribosomal gene sequencing and Molecular Evolutionary Genetics Analysis version 7. The results demonstrated that Escherichia (33.3%, 37/111), Clostridium (24.3%, 27/111) and Enterococcus (11.7%, 13/111) exhibited a high ratio in asymptomatic patients. These isolates derived from two phyla: Firmicutes (51.3%, 57/111) and Proteobacteria (44.1%, 49/111). In addition, co-colonization of human pathogens Fusobacterium nucleatum, Ralstonia pickettii, Klebsiella pneumoniae, Klebsiella quasipneumoniae and Clostridium tertium with C. difficile was identified. To the best of our knowledge, these pathogens have not been co-isolated with C. difficile previously. CONCLUSIONS: In summary, the present study identified the composition of fecal microbiota in two asymptomatic patients in Guizhou, China. These results suggested that co-infection with human pathogens may be ubiquitous during CDI progression.
BACKGROUND: Clostridium difficile infection (CDI) is the leading cause of nosocomial diarrhea. Co-colonization of key bacterial taxa may prevent the transition from asymptomatic C. difficile colonization to CDI. However, little is known about the composition of key bacterial taxa in asymptomatic patients. METHODS: In the present study, the culture method was used to examine the composition of stool microbiota in two asymptomatic patients from Guizhou, China. RESULTS: A total of 111 strains were isolated and phylogenetic relationships were determined by 16S ribosomal gene sequencing and Molecular Evolutionary Genetics Analysis version 7. The results demonstrated that Escherichia (33.3%, 37/111), Clostridium (24.3%, 27/111) and Enterococcus (11.7%, 13/111) exhibited a high ratio in asymptomatic patients. These isolates derived from two phyla: Firmicutes (51.3%, 57/111) and Proteobacteria (44.1%, 49/111). In addition, co-colonization of human pathogens Fusobacterium nucleatum, Ralstonia pickettii, Klebsiella pneumoniae, Klebsiella quasipneumoniae and Clostridium tertium with C. difficile was identified. To the best of our knowledge, these pathogens have not been co-isolated with C. difficile previously. CONCLUSIONS: In summary, the present study identified the composition of fecal microbiota in two asymptomatic patients in Guizhou, China. These results suggested that co-infection with human pathogens may be ubiquitous during CDI progression.
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