Lin-Lin Tian1,2, Bin Qian3, Xiao-Hui Jiang4, Yu-Shan Liu5, Tong Chen6, Cheng-You Jia7, Ya-Li Zhou2, Ji-Bin Liu4, Yu-Shui Ma7, Da Fu7, Sen-Tai Ding1. 1. Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China. 2. Department of Microbiology, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541001, China. 3. Department of General Surgery, Shanghai Eighth People's Hospital, Shanghai 200235, China. 4. Department of Gastrointestinal Surgery, Affiliated Tumor Hospital of Nantong University, Nantong 226631, China. 5. Department of Pathology, Nantong Tumor Hospital, Nantong 226631, China. 6. Department of Pediatric Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University, China. 7. Central Laboratory for Medical Research, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
Abstract
BACKGROUND: MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. METHODS: In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. RESULTS: The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. CONCLUSION: The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.
BACKGROUND: MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. METHODS: In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. RESULTS: The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. CONCLUSION: The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.
Authors: Beilei Zhang; Xinan Wang; Jiacong Deng; Haifeng Zheng; Wei Liu; Si Chen; Jie Tian; Fu Wang Journal: Cancer Lett Date: 2019-06-01 Impact factor: 8.679
Authors: Daniela Rodrigues; Terezinha Souza; Danyel G J Jennen; Lieve Lemmens; Jos C S Kleinjans; Theo M de Kok Journal: Cancer Treat Rev Date: 2019-06-27 Impact factor: 12.111
Authors: Dean A Fergusson; Neil L Wesch; Garvin J Leung; Jenna L MacNeil; Isidora Conic; Justin Presseau; Kelly D Cobey; Jean-Simon Diallo; Rebecca Auer; Jonathan Kimmelman; Natasha Kekre; Nader El-Sayes; Ramya Krishnan; Brian A Keller; Carolina Ilkow; Manoj M Lalu Journal: Mol Ther Oncolytics Date: 2019-05-21 Impact factor: 7.200