Gabriela Santana de Paula1, Mateus Cardoso Oliveira2, Luciana Solera Sales3, Marcelo Boriollo4, Lidiany Karla Azevedo Rodrigues5, Marinês Nobre-Dos-Santos6, Carolina Steiner-Oliveira7. 1. Department of Health Sciences and Pediatric Dentistry, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: gabispaula@hotmail.com. 2. Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: mateus_oc1@hotmail.com. 3. Department of Health Sciences and Pediatric Dentistry, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: Luciana_9891@hotmail.com. 4. Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: marcelofgb@yahoo.com.br. 5. Department of Operative Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Zip Code: 60430-170, Brazil. Electronic address: lidianykarla@ufc.br. 6. Department of Health Sciences and Pediatric Dentistry, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: mnobre@unicamp.br. 7. Department of Health Sciences and Pediatric Dentistry, Piracicaba Dental School, University of Campinas, UNICAMP, Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil. Electronic address: csteiner@unicamp.br.
Abstract
OBJECTIVE: To test the effect of antimicrobial photodynamic therapy (A-PDT) on the oral biofilm formed with early colonizing microorganisms, using the photosensitizer methylene blue coupled with β-cyclodextrin nanoparticles and red light sources laser or LED (λ =660 nm). METHODS: The groups were divided into (n = 3, in triplicate): C (negative control, 0.9 % NaCl), CX (positive control, 0.2 % chlorhexidine), P (Photosensitizer/Nanoparticle), L (Laser), LED (light-emitting diode), LP (Laser + Photosensitizer/Nanoparticle) and LEDP (LED + Photosensitizer/Nanoparticle). A multispecies biofilm composed ofS. gordonii, S. oralis, S. mitis, and S. sanguinis was grown in microplates containing BHI supplemented with 1% sucrose (w/v) for 24 h. Light irradiations were applied with a laser at 9 J for 90 s (320 J/cm2), or with LED, at 8.1 J for 90 s (8.1 J/cm2). The microbial reduction was assessed by counting viable biofilm microorganisms in selective culture media, before and after the treatments. Data normality was assessed by the Shapiro-Wilk test, and the results were submitted to Kruskal-Wallis analysis, followed by Dunn's test, with a significance level of 5%. RESULTS: The groups LP and LEDP were able to significantly reduce the biofilm microorganism counts by as much as 4 log10 times compared to the negative control group (p < 0.05) and did not statistically differ from the positive control group (CX) (p > 0.05). CONCLUSION: The A-PDT mediated by encapsulated β-cyclodextrin methylene blue irradiated by Laser or LED was effective in the microbial reduction of multispecies biofilm composed of early colonizing microorganisms.
OBJECTIVE: To test the effect of antimicrobial photodynamic therapy (A-PDT) on the oral biofilm formed with early colonizing microorganisms, using the photosensitizer methylene blue coupled with β-cyclodextrin nanoparticles and red light sources laser or LED (λ =660 nm). METHODS: The groups were divided into (n = 3, in triplicate): C (negative control, 0.9 % NaCl), CX (positive control, 0.2 % chlorhexidine), P (Photosensitizer/Nanoparticle), L (Laser), LED (light-emitting diode), LP (Laser + Photosensitizer/Nanoparticle) and LEDP (LED + Photosensitizer/Nanoparticle). A multispecies biofilm composed ofS. gordonii, S. oralis, S. mitis, and S. sanguinis was grown in microplates containing BHI supplemented with 1% sucrose (w/v) for 24 h. Light irradiations were applied with a laser at 9 J for 90 s (320 J/cm2), or with LED, at 8.1 J for 90 s (8.1 J/cm2). The microbial reduction was assessed by counting viable biofilm microorganisms in selective culture media, before and after the treatments. Data normality was assessed by the Shapiro-Wilk test, and the results were submitted to Kruskal-Wallis analysis, followed by Dunn's test, with a significance level of 5%. RESULTS: The groups LP and LEDP were able to significantly reduce the biofilm microorganism counts by as much as 4 log10 times compared to the negative control group (p < 0.05) and did not statistically differ from the positive control group (CX) (p > 0.05). CONCLUSION: The A-PDT mediated by encapsulated β-cyclodextrin methylene blue irradiated by Laser or LED was effective in the microbial reduction of multispecies biofilm composed of early colonizing microorganisms.