Naruhito Iwasaki1,2, Seigo Terawaki3, Kouhei Shimizu3, Daisuke Oikawa3, Hirokazu Sakamoto4, Kishiko Sunami4, Fuminori Tokunaga3. 1. Department of Pathobiochemistry, Graduate School of Medicine, Osaka City University, Osaka, Japan. m2071288@med.osaka-cu.ac.jp. 2. Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Osaka City University, Osaka, Japan. m2071288@med.osaka-cu.ac.jp. 3. Department of Pathobiochemistry, Graduate School of Medicine, Osaka City University, Osaka, Japan. 4. Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Osaka City University, Osaka, Japan.
Abstract
OBJECTIVE: Histamine derived from mast cells and basophils plays important roles in inducing allergic symptoms. Although T cells also produce histamine, the involvement of the histamine produced from T cells has remained enigmatic. We sought to reveal the roles of T helper 2 (Th2) cell-derived histamine in nasal allergic disorders. METHODS: The histamine production from Th2 cells was measured by EIA. The mRNA expression of histidine decarboxylase (HDC) was measured by real-time PCR. To investigate the roles of Th2 cell-derived histamine in vivo, we analyzed an antigen-specific Th2 cell transfer mouse model. RESULTS: Th2 cells produced histamine by T cell receptor stimulation, and these properties were specific for Th2 cells, but not Th1 cells and naïve CD4 T cells. The histamine produced from Th2 cells was involved in the infiltrations of Th2 cells in response to antigen exposure. CONCLUSION: These results suggest that Th2 cell-derived histamine play important roles in nasal allergic disorders.
OBJECTIVE:Histamine derived from mast cells and basophils plays important roles in inducing allergic symptoms. Although T cells also produce histamine, the involvement of the histamine produced from T cells has remained enigmatic. We sought to reveal the roles of T helper 2 (Th2) cell-derived histamine in nasal allergic disorders. METHODS: The histamine production from Th2 cells was measured by EIA. The mRNA expression of histidine decarboxylase (HDC) was measured by real-time PCR. To investigate the roles of Th2 cell-derived histamine in vivo, we analyzed an antigen-specific Th2 cell transfer mouse model. RESULTS:Th2 cells produced histamine by T cell receptor stimulation, and these properties were specific for Th2 cells, but not Th1 cells and naïve CD4 T cells. The histamine produced from Th2 cells was involved in the infiltrations of Th2 cells in response to antigen exposure. CONCLUSION: These results suggest that Th2 cell-derived histamine play important roles in nasal allergic disorders.
Authors: Mohamed A Morsy; Snehal S Patel; Anita Bakrania; Mahmoud Kandeel; Anroop B Nair; Jigar N Shah; Sabah H Akrawi; Mahmoud El-Daly Journal: Life (Basel) Date: 2022-05-31