Anderson Apolonio Silva Pedroza1, Elenilson M Bernardo1, Allifer R Pereira2, Severina Cassia Andrade Silva3, Talitta A Lima2, Cristiane de Moura Freitas2, Jose Carlos da Silva Junior3, Dayane A Gomes3, Diorginis S Ferreira4, Claudia J Lagranha5. 1. Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, PE, Brazil. 2. Laboratory of Biochemistry and Exercise Biochemistry, Department of Physical Education and Sports Science, CAV- Federal University of Pernambuco, Brazil. 3. Neuropsyquiatry and Behavior Science Graduate Program, Federal University of Pernambuco-UFPE, Recife, Pernambuco, Brazil. 4. Colegiado de Educação Física, Federal University of São Franscisco Valley, Petrolina, Brazil. 5. Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, PE, Brazil; Laboratory of Biochemistry and Exercise Biochemistry, Department of Physical Education and Sports Science, CAV- Federal University of Pernambuco, Brazil; Neuropsyquiatry and Behavior Science Graduate Program, Federal University of Pernambuco-UFPE, Recife, Pernambuco, Brazil. Electronic address: lagranha@hotmail.com.
Abstract
BACKGROUND AND AIMS: It has been demonstrated that maternal low protein during development induces mitochondrial dysfunction and oxidative stress in the heart. Moderate-intensity exercise in early life, conversely, increases the overall cardiac health. Thus, we hypothesize that moderate-intensity exercise performed during young age could ameliorate the deleterious effect of maternal protein deprivation on cardiac bioenergetics. METHODS AND RESULTS: We used a rat model of maternal protein restriction during gestational and lactation period followed by an offspring treadmill moderate physical training. Pregnant rats were divided into two groups: normal nutrition receiving 17% of casein in the diet and undernutrition receiving a low-protein diet (8% casein). At 30 days of age, the male offspring were further subdivided into sedentary (NS and LS) or exercised (NT and LT) groups. Treadmill exercise was performed as follows: 4 weeks, 5 days/week, 60 min/day at 50% of maximal running capacity. Our results showed that a low-protein diet decreases oxidative metabolism and mitochondrial function associated with higher oxidative stress. In contrast, exercise rescues mitochondrial capacity and promotes a cellular resilience to oxidative stress. Up-regulation of cardiac sirtuin 1 and 3 decreased acetylation levels, redeeming from the deleterious effect of protein restriction. CONCLUSION: Our findings show that moderate daily exercise during a young age acts as a therapeutical intervention opposing the harmful effects of a maternal diet restricted in protein.
BACKGROUND AND AIMS: It has been demonstrated that maternal low protein during development induces mitochondrial dysfunction and oxidative stress in the heart. Moderate-intensity exercise in early life, conversely, increases the overall cardiac health. Thus, we hypothesize that moderate-intensity exercise performed during young age could ameliorate the deleterious effect of maternal protein deprivation on cardiac bioenergetics. METHODS AND RESULTS: We used a rat model of maternal protein restriction during gestational and lactation period followed by an offspring treadmill moderate physical training. Pregnant rats were divided into two groups: normal nutrition receiving 17% of casein in the diet and undernutrition receiving a low-protein diet (8% casein). At 30 days of age, the male offspring were further subdivided into sedentary (NS and LS) or exercised (NT and LT) groups. Treadmill exercise was performed as follows: 4 weeks, 5 days/week, 60 min/day at 50% of maximal running capacity. Our results showed that a low-protein diet decreases oxidative metabolism and mitochondrial function associated with higher oxidative stress. In contrast, exercise rescues mitochondrial capacity and promotes a cellular resilience to oxidative stress. Up-regulation of cardiac sirtuin 1 and 3 decreased acetylation levels, redeeming from the deleterious effect of protein restriction. CONCLUSION: Our findings show that moderate daily exercise during a young age acts as a therapeutical intervention opposing the harmful effects of a maternal diet restricted in protein.