Literature DB >> 3380691

The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

K A Jacobs1, R Rudersdorf, S D Neill, J P Dougherty, E L Brown, E F Fritsch.   

Abstract

In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.

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Year:  1988        PMID： 3380691      PMCID： PMC336654          DOI： 10.1093/nar/16.10.4637

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

Review 1.  Hybridization and renaturation kinetics of nucleic acids.

Authors:  J G Wetmur
Journal:  Annu Rev Biophys Bioeng       Date:  1976

2.  THE MELTING TRANSITION OF LOW-MOLECULAR-WEIGHT DNA: THEORY AND EXPERIMENT.

Authors:  D M CROTHERS; N R KALLENBACH; B H ZIMM
Journal:  J Mol Biol       Date:  1965-04       Impact factor: 5.469

3.  DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions.

Authors:  J M Orosz; J G Wetmur
Journal:  Biopolymers       Date:  1977-06       Impact factor: 2.505

4.  Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA.

Authors:  C T Chang; T C Hain; J R Hutton; J G Wetmur
Journal:  Biopolymers       Date:  1974       Impact factor: 2.505

5.  Analysis of repeating DNA sequences by reassociation.

Authors:  R J Britten; D E Graham; B R Neufeld
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.

Authors:  R J Britten; A Cetta; E H Davidson
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

7.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

8.  Dependence of the melting temperature of DNA on salt concentration.

Authors:  C Schildkraut
Journal:  Biopolymers       Date:  1965       Impact factor: 2.505

9.  Alteration of the relative stability of dA-dT and dG-dC base pairs in DNA.

Authors:  W B Melchior; P H Von Hippel
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

10.  Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Authors:  R B Wallace; J Shaffer; R F Murphy; J Bonner; T Hirose; K Itakura
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971
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  33 in total

1.  New specificity and yield enhancer of polymerase chain reactions.

Authors:  M Kovárová; P Dráber
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

2.  Gene expression analysis with universal n-mer arrays.

Authors:  R Michael van Dam; Stephen R Quake
Journal:  Genome Res       Date:  2002-01       Impact factor: 9.043

3.  Modification of DNA duplexes to smooth their thermal stability independently of their base content for DNA sequencing by hybridization.

Authors:  H K Nguyen; P Auffray; U Asseline; D Dupret; N T Thuong
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

4.  Molecular cloning of a candidate chicken prion protein.

Authors:  J M Gabriel; B Oesch; H Kretzschmar; M Scott; S B Prusiner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-01       Impact factor: 11.205

5.  Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts.

Authors:  J A Gogos; M Karayiorgou; H Aburatani; F C Kafatos
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

6.  Analysis of a cDNA encoding arginine decarboxylase from oat reveals similarity to the Escherichia coli arginine decarboxylase and evidence of protein processing.

Authors:  E Bell; R L Malmberg
Journal:  Mol Gen Genet       Date:  1990-12

7.  Analysis of microRNA expression by in situ hybridization with RNA oligonucleotide probes.

Authors:  Robert C Thompson; Monika Deo; David L Turner
Journal:  Methods       Date:  2007-10       Impact factor: 3.608

8.  DNA sequencing by hybridization: 100 bases read by a non-gel-based method.

Authors:  Z Strezoska; T Paunesku; D Radosavljević; I Labat; R Drmanac; R Crkvenjakov
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

9.  Identification of vaccine-related polioviruses by hybridization with specific RNA probes.

Authors:  L De; B Nottay; C F Yang; B P Holloway; M Pallansch; O Kew
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

10.  Cat scratch disease: the rare role of Afipia felis.

Authors:  M Giladi; B Avidor; Y Kletter; S Abulafia; L N Slater; D F Welch; D J Brenner; A G Steigerwalt; A M Whitney; M Ephros
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948
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