Francesca Oliviero1, Paola Galozzi1, Anna Scanu1, Francesca Galuppini2, Vanni Lazzarin2, Silvia Brocco3, Giampietro Ravagnan4, Paolo Sfriso1, Roberta Ramonda1, Paolo Spinella5, Leonardo Punzi6, Gianmaria Pennelli2, Roberto Luisetto7. 1. Rheumatology Unit, Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy. 2. Surgical Pathology Unit, Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy. 3. Radiology Unit, Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy. 4. Institute of Translational Pharmacology-National Research Council, 00133 Rome, Italy. 5. Clinical Nutrition Unit, Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy. 6. Centre for Gout and Metabolic Bone and Joint Diseases, Rheumatology, SS Giovanni and Paolo Hospital, 30122 Venice, Italy. 7. Department of Surgery, Oncology and Gastroenterology-DISCOG, University of Padova, 35128 Padova, Italy.
Abstract
BACKGROUND: Polydatin is a stilbenoid with important antioxidant, anti-inflammatory, and immunomodulating properties. The aim of this study was to assess the anti-inflammatory preventive effect of polydatin in the mouse model of acute arthritis induced by calcium pyrophosphate (CPP) crystals. METHODS: Acute arthritis was induced by the injection of a suspension of sterile CPP crystals into the ankle joint of Balb/c mice. Animals were randomized to receive polydatin or colchicine (the control drug) according to a prophylactic and a therapeutic protocol. The primary outcome was the variation of ankle swelling obtained after crystal injection and treatment, while histological parameters such as leukocyte infiltration, IL-1ß and CXCL1 levels and tissue expression were considered as secondary outcomes. RESULTS: Prophylactic treatment with PD significantly diminished ankle swelling after 48 h from crystal injection. Secondary outcomes such as leukocyte infiltration, necrosis, edema, and synovitis were also decreased. PD caused a reduction in circulating levels of IL-1ß and CXCL1, as well as their tissue expression. By contrast, the therapeutic administration of PD did not have any beneficial effect. CONCLUSIONS: PD can effectively prevent acute inflammatory response to crystals in the mouse model of CPP crystal-induced arthritis. These results suggest that this bioactive compound might be used in the prevention of crystal-induced acute attacks in humans.
BACKGROUND:Polydatin is a stilbenoid with important antioxidant, anti-inflammatory, and immunomodulating properties. The aim of this study was to assess the anti-inflammatory preventive effect of polydatin in the mouse model of acute arthritis induced by calcium pyrophosphate (CPP) crystals. METHODS:Acute arthritis was induced by the injection of a suspension of sterile CPP crystals into the ankle joint of Balb/c mice. Animals were randomized to receive polydatin or colchicine (the control drug) according to a prophylactic and a therapeutic protocol. The primary outcome was the variation of ankle swelling obtained after crystal injection and treatment, while histological parameters such as leukocyte infiltration, IL-1ß and CXCL1 levels and tissue expression were considered as secondary outcomes. RESULTS: Prophylactic treatment with PD significantly diminished ankle swelling after 48 h from crystal injection. Secondary outcomes such as leukocyte infiltration, necrosis, edema, and synovitis were also decreased. PD caused a reduction in circulating levels of IL-1ß and CXCL1, as well as their tissue expression. By contrast, the therapeutic administration of PD did not have any beneficial effect. CONCLUSIONS: PD can effectively prevent acute inflammatory response to crystals in the mouse model of CPPcrystal-induced arthritis. These results suggest that this bioactive compound might be used in the prevention of crystal-induced acute attacks in humans.
Authors: W Zhang; M Doherty; E Pascual; V Barskova; P-A Guerne; T L Jansen; B F Leeb; F Perez-Ruiz; J Pimentao; L Punzi; P Richette; F Sivera; T Uhlig; I Watt; T Bardin Journal: Ann Rheum Dis Date: 2011-01-20 Impact factor: 19.103
Authors: Francesca Oliviero; Yessica Zamudio-Cuevas; Elisa Belluzzi; Lisa Andretto; Anna Scanu; Marta Favero; Roberta Ramonda; Giampietro Ravagnan; Alberto López-Reyes; Paolo Spinella; Leonardo Punzi Journal: Foods Date: 2019-11-07