Jie Liu1, Chao-An Chen2, Xiaofei Zhu3, Brian R Morrow4, Ukrit Thamma5, Tia J Kowal6, Hassan M Moawad7, Matthias M Falk8, Himanshu Jain9, George T-J Huang10. 1. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States. Electronic address: liu.7050@osu.edu. 2. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States; Department of Endodontics, Chi Mei Medical Center, Liouying, Tainan, Taiwan; Department of Endodontics, Chi Mei Medical Center, Yongkang, Tainan, Taiwan. Electronic address: hardstone49@gmail.com. 3. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States; VIP Dental Service and Geriatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, China. Electronic address: zhuxiaofei767@foxmail.com. 4. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States. Electronic address: morrow@uthsc.edu. 5. Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA, 18015, United States. Electronic address: ukritthamma@gmail.com. 6. Department of Biological Sciences, Lehigh University, Bethlehem, PA, 18015, United States. Electronic address: tjkowal317@gmail.com. 7. Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA, 18015, United States. Electronic address: hmm205@lehigh.edu. 8. Department of Biological Sciences, Lehigh University, Bethlehem, PA, 18015, United States. Electronic address: mmf4@lehigh.edu. 9. Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA, 18015, United States. Electronic address: h.jain@lehigh.edu. 10. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States. Electronic address: gtjhuang@uthsc.edu.
Abstract
INTRODUCTION/ OBJECTIVE: The tailored amorphous multi-porous (TAMP) material fabrication technology has led to a new class of bioactive materials possessing versatile characteristics. It has not been tested for dental applications. Thus, we aimed to assess its biocompatibility and ability to regenerate dental mineral tissue. METHODS: 30CaO-70SiO2 model TAMP discs were fabricated by a sol-gel method followed by in vitro biocompatibility testing with isolated human or mini-swine dental pulp stem cells (DPSCs). TAMP scaffolds were tested in vivo as a pulp exposure (pin-point, 1 mm, 2 mm, and entire pulp chamber roof) capping material in the molar teeth of mini-swine. RESULTS: The in vitro assays showed that DPSCs attached well onto the TAMP discs with comparable viability to those attached to culture plates. Pulp capping tests on mini-swine showed that after 4.5 months TAMP material was still present at the capping site, and mineral tissue (dentin bridge) had formed in all sizes of pulp exposure underneath the TAMP material. CONCLUSIONS: TAMP calcium silicate is biocompatible with both human and swine DPSCs in vitro and with pulp in vivo, it may help regenerate the dentin bridge after pulp exposure.
INTRODUCTION/ OBJECTIVE: The tailored amorphous multi-porous (TAMP) material fabrication technology has led to a new class of bioactive materials possessing versatile characteristics. It has not been tested for dental applications. Thus, we aimed to assess its biocompatibility and ability to regenerate dental mineral tissue. METHODS: 30CaO-70SiO2 model TAMP discs were fabricated by a sol-gel method followed by in vitro biocompatibility testing with isolated human or mini-swine dental pulp stem cells (DPSCs). TAMP scaffolds were tested in vivo as a pulp exposure (pin-point, 1 mm, 2 mm, and entire pulp chamber roof) capping material in the molar teeth of mini-swine. RESULTS: The in vitro assays showed that DPSCs attached well onto the TAMP discs with comparable viability to those attached to culture plates. Pulp capping tests on mini-swine showed that after 4.5 months TAMP material was still present at the capping site, and mineral tissue (dentin bridge) had formed in all sizes of pulp exposure underneath the TAMP material. CONCLUSIONS: TAMP calcium silicate is biocompatible with both human and swine DPSCs in vitro and with pulp in vivo, it may help regenerate the dentin bridge after pulp exposure.
Authors: S Wang; M M Falk; A Rashad; M M Saad; A C Marques; R M Almeida; M K Marei; H Jain Journal: J Mater Sci Mater Med Date: 2011-03-29 Impact factor: 3.896
Authors: Henri Granel; Cédric Bossard; Lisa Nucke; Fabien Wauquier; Gael Y Rochefort; Jérôme Guicheux; Edouard Jallot; Jonathan Lao; Yohann Wittrant Journal: Adv Healthc Mater Date: 2019-04-03 Impact factor: 9.933
Authors: Luiz Felipe Cardoso Lehman; Mariana Saturnino de Noronha; Ivana Márcia Alves Diniz; Rosangela Maria Ferreira da Costa E Silva; Ângela Leão Andrade; Luiz Fernando de Sousa Lima; Carlos Eduardo Pinto de Alcântara; Rosana Domingues; Anderson José Ferreira; Tarcília Aparecida da Silva; Ricardo Alves Mesquita Journal: J Tissue Eng Regen Med Date: 2019-07-10 Impact factor: 3.963