Literature DB >> 33796622

Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation.

Jinyun Wu1, Yuqi Cai1, Xiaoyang Wu1, Yue Ying1, Yilin Tai1, Miao He1.   

Abstract

Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Aorta; Brain; Mouse; Paraformaldehyde; Transcardiac perfusion

Year:  2021        PMID： 33796622      PMCID： PMC8005872          DOI： 10.21769/BioProtoc.3988

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  1 in total

1.  Whole animal perfusion fixation for rodents.

Authors:  Gregory J Gage; Daryl R Kipke; William Shain
Journal:  J Vis Exp       Date:  2012-07-30       Impact factor: 1.355
  1 in total
  4 in total

1.  A Retro-orbital Sinus Injection Mouse Model to Study Early Events and Reorganization of the Astrocytic Network during Pneumococcal Meningitis.

Authors:  Chakir Bello; Martine Cohen-Salmon; Guy Tran Van Nhieu
Journal:  Bio Protoc       Date:  2021-12-05

2.  Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata.

Authors:  Alexandra Alvarsson; Maria Jimenez-Gonzalez; Rosemary Li; Carolina Rosselot; Nikolaos Tzavaras; Zhuhao Wu; Sarah A Stanley
Journal:  Bio Protoc       Date:  2021-08-05

3.  Perfusion with 10% neutral-buffered formalin is equivalent to 4% paraformaldehyde for histopathology and immunohistochemistry in a mouse model of experimental autoimmune encephalomyelitis.

Authors:  Jessica M Snyder; Enrico Radaelli; Adam Goeken; Thomas Businga; Alexander W Boyden; Nitin J Karandikar; Katherine N Gibson-Corley
Journal:  Vet Pathol       Date:  2022-02-07       Impact factor: 3.157

4.  Co-registration of mouse cortical structures between in vivo and ex vivo images using tangential sectioning.

Authors:  Naveen Ouellette; Emily Turschak; Jun Zhuang
Journal:  STAR Protoc       Date:  2022-05-19
  4 in total

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