Effect of Tris-extender supplemented with a combination of turmeric and ethylene glycol on buffalo bull semen freezability and in vivo fertility.

The objective of this study was to evaluate the effect of Tris-extender supplemented with a combination of turmeric and ethylene glycol on buffalo bull semen preservability and in vivo fertility. Five tubes (each contain 5 ml Tris-citric acid-fructose yolk, TCFY). The first tube contains turmeric extract and ethylene glycol and kept as a control. The other four tubes contain 1.5% ethylene glycol plus turmeric extract as follows: 100 μl/5 ml, 200 μl/5 ml, 300 μl/5 ml, and 400 μl/5 ml v/v). Pooled bull semen was added to the Tris extender and to Tris turmeric ethylene glycol. Extended semen was subjected to semen freezing protocol. Semen assessment was carried out. In Tris turmeric ethylene glycol post-cooling, sperm motility and alive sperms were significantly higher in the first concentration of Tris turmeric ethylene glycol (TTE1), sperm abnormalities were significantly lower in TTE1 and TTE2, and sperm membrane integrity (HOST) was significantly higher in TTE1 and acrosome percent was significantly higher in TTE1, TTE2, and TTE4 if compared to the control. Post-thawing, sperm motility was significantly higher in TTE1 as compared to the control and other concentrations of TE. All concentrations of TE were significantly higher in sperm membrane integrity (HOST) as relative to the control. Alive sperms were kept in TTE1 as the control. Acrosome percent was kept in most concentrations as the control. It is concluded that, in cooled and post-thawed semen, the first concentration (TTE1) gave the best sperm quality and conception rate.