Literature DB >> 33791982

Methods of Lipidomic Analysis: Extraction, Derivatization, Separation, and Identification of Lipids.

Ya Xie1,2, Zongyuan Wu1,2, Zuojian Qin1,2, Bangfu Wu1,2, Xin Lv1,2, Fang Wei1,2, Hong Chen3,4.   

Abstract

Lipidomics refers to the large-scale study of pathways and networks of cellular lipids in biological systems. A lipidomic analysis often involves the identification and quantification of the thousands of cellular lipid molecular species within a complex biological sample and therefore requires a well optimized method for lipid profiling. In this chapter, the methods for lipidomic analysis, including sample collection and preparation, lipid derivatization and separation, mass spectrometric identification of lipids, data processing and interpretation, and quality control, are overviewed.

Entities:  

Keywords:  Chromatographic separation of lipids; Lipid derivatization; Lipid extraction; Lipidomics; Mass spectrometry

Mesh:

Substances:

Year:  2021        PMID: 33791982     DOI: 10.1007/978-3-030-51652-9_12

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN: 0065-2598            Impact factor:   2.622


  87 in total

1.  Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer.

Authors:  Hye Kyeong Min; Sangsoo Lim; Bong Chul Chung; Myeong Hee Moon
Journal:  Anal Bioanal Chem       Date:  2010-10-17       Impact factor: 4.142

2.  Comprehensive analysis of the major lipid classes in sebum by rapid resolution high-performance liquid chromatography and electrospray mass spectrometry.

Authors:  Emanuela Camera; Matteo Ludovici; Marisa Galante; Jo-Linda Sinagra; Mauro Picardo
Journal:  J Lipid Res       Date:  2010-08-17       Impact factor: 5.922

3.  Lipid and lipid mediator profiling of human synovial fluid in rheumatoid arthritis patients by means of LC-MS/MS.

Authors:  Martin Giera; Andreea Ioan-Facsinay; Rene Toes; Fei Gao; Jesmond Dalli; André M Deelder; Charles N Serhan; Oleg A Mayboroda
Journal:  Biochim Biophys Acta       Date:  2012-07-24

4.  Stop-flow comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection for phospholipid analysis.

Authors:  Paola Dugo; Nermeen Fawzy; Filomena Cichello; Francesco Cacciola; Paola Donato; Luigi Mondello
Journal:  J Chromatogr A       Date:  2012-12-28       Impact factor: 4.759

5.  Rapid profiling and quantification of phospholipid molecular species in human plasma based on chemical derivatization coupled with electrospray ionization tandem mass spectrometry.

Authors:  Fang Wei; Xiang Wang; Hui-Fang Ma; Xin Lv; Xu-Yan Dong; Hong Chen
Journal:  Anal Chim Acta       Date:  2018-04-18       Impact factor: 6.558

6.  Profiling and relative quantification of phosphatidylethanolamine based on acetone stable isotope derivatization.

Authors:  Xiang Wang; Fang Wei; Ji-Qu Xu; Xin Lv; Xu-Yan Dong; Xianlin Han; Siew-Young Quek; Feng-Hong Huang; Hong Chen
Journal:  Anal Chim Acta       Date:  2015-11-17       Impact factor: 6.558

7.  Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis.

Authors:  Hieu Sy Vu; Sunitha Shiva; Mary R Roth; Pamela Tamura; Lianqing Zheng; Maoyin Li; Sujon Sarowar; Samuel Honey; Dedan McEllhiney; Paul Hinkes; Lawrence Seib; Todd D Williams; Gary Gadbury; Xuemin Wang; Jyoti Shah; Ruth Welti
Journal:  Plant J       Date:  2014-10-03       Impact factor: 6.417

8.  Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry.

Authors:  Maoyin Li; Ethan Baughman; Mary R Roth; Xianlin Han; Ruth Welti; Xuemin Wang
Journal:  Plant J       Date:  2013-11-29       Impact factor: 6.417

9.  Membrane glycerolipidome of soybean root hairs and its response to nitrogen and phosphate availability.

Authors:  Fang Wei; Brian Fanella; Liang Guo; Xuemin Wang
Journal:  Sci Rep       Date:  2016-11-04       Impact factor: 4.379

10.  Serum metabolome and lipidome changes in adult patients with primary dengue infection.

Authors:  Liang Cui; Yie Hou Lee; Yadunanda Kumar; Fengguo Xu; Kun Lu; Eng Eong Ooi; Steven R Tannenbaum; Choon Nam Ong
Journal:  PLoS Negl Trop Dis       Date:  2013-08-15
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.