Xiaolin Zhong1,2, Ou Chen3, TieJun Zhou4, Muhan Lü1,2, Juyi Wan5,6,7. 1. Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. 2. Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, China. 3. Department of Gastroenterology, Ya'an People's Hospital, Ya'an 625000, China. 4. Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. 5. Department of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. 6. Cardiovascular and Metabolic Diseases Key Laboratory of Luzhou, Luzhou 646000, China. 7. Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases) Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, China.
Abstract
OBJECTIVES: To investigate the effects of cytotoxin-associated gene A- (CagA-) positive Helicobacter pylori on proliferation, invasion, autophagy, and expression of miR-125b-5p in colon cancer cells. METHODS: Colon cancer cells were cocultured with H. pylori (CagA+) to analyze the effects of H. pylori on miR-125b-5p and autophagy. Colon cancer cells infected with H. pylori (CagA+) were mimicked by transfection of CagA plasmid. The effects of CagA on the proliferation, invasion, and autophagy of colon cancer cells were analyzed. Cell counting kit-8 (CCK-8), clone formation, and Transwell assays were used to detect cell viability, proliferation, and invasion ability, respectively. Proteins and miRNAs were detected by western blotting and qPCR, respectively. RESULTS: H. pylori (CagA+) inhibited expression of miR-125b-5p and promoted autophagy in colon cancer cells. MiR-125 b-5p was underexpressed in colon cancer cells after CagA overexpression. CagA promoted colon cancer cell proliferation, invasion, and autophagy. Overexpression of miR-125b-5p inhibited the proliferation, invasion, and autophagy of colon cancer cells and reversed the effects of CagA. CONCLUSION: H. pylori (CagA+) infection may promote the development and invasion of colon cancer by inhibiting miR-125b-5p.
OBJECTIVES: To investigate the effects of cytotoxin-associated gene A- (CagA-) positive Helicobacter pylori on proliferation, invasion, autophagy, and expression of miR-125b-5p in colon cancer cells. METHODS: Colon cancer cells were cocultured with H. pylori (CagA+) to analyze the effects of H. pylori on miR-125b-5p and autophagy. Colon cancer cells infected with H. pylori (CagA+) were mimicked by transfection of CagA plasmid. The effects of CagA on the proliferation, invasion, and autophagy of colon cancer cells were analyzed. Cell counting kit-8 (CCK-8), clone formation, and Transwell assays were used to detect cell viability, proliferation, and invasion ability, respectively. Proteins and miRNAs were detected by western blotting and qPCR, respectively. RESULTS: H. pylori (CagA+) inhibited expression of miR-125b-5p and promoted autophagy in colon cancer cells. MiR-125 b-5p was underexpressed in colon cancer cells after CagA overexpression. CagA promoted colon cancer cell proliferation, invasion, and autophagy. Overexpression of miR-125b-5p inhibited the proliferation, invasion, and autophagy of colon cancer cells and reversed the effects of CagA. CONCLUSION: H. pylori (CagA+) infection may promote the development and invasion of colon cancer by inhibiting miR-125b-5p.
Authors: Nianshuang Li; Yan Feng; Yi Hu; Cong He; Chuan Xie; Yaobin Ouyang; Stephen C Artim; Deqiang Huang; Yin Zhu; Zhijun Luo; Zhongming Ge; Nonghua Lu Journal: J Exp Clin Cancer Res Date: 2018-11-22
Authors: Yu Wang; Jiang Du; Xuemei Wu; Ahmed Abdelrehem; Yu Ren; Chao Liu; Xuan Zhou; Sinan Wang Journal: Mol Cancer Date: 2021-12-11 Impact factor: 27.401