Literature DB >> 33786775

A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors.

Luiza Ghila1, Thomas Aga Legøy1, Simona Chera2.   

Abstract

Pancreatic islet endocrine cells generated from patient-derived induced pluripotent stem cells represent a great strategy for both disease modeling and regenerative medicine. Nevertheless, these cells inherently miss the effects of the intricate network of systemic signals characterizing the living organisms. Xenotransplantation of in vitro differentiating cells into murine hosts substantially compensates for this drawback.Here we describe our transplantation strategy of encapsulated differentiating pancreatic progenitors into diabetic immunosuppressed (NSG) overtly diabetic mice generated by the total ablation of insulin-producing cells following diphtheria toxin administration. We will detail the differentiation protocol employed, the alginate encapsulation procedure, and the xenotransplantation steps required for a successful and reproducible experiment.
© 2021. Springer Science+Business Media, LLC.

Entities:  

Keywords:  Alginate beads; Diabetic mouse model; Encapsulation; Human iPSC; In vitro differentiation; Islet endocrine cells; Pancreatic endocrine progenitors; RIP-DTR; Xenotransplantation

Mesh：

Substances：

Year:  2022        PMID： 33786775     DOI： 10.1007/7651_2021_356

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  33 in total

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1.  Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells.

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