Literature DB >> 3378459

Evaluation of four methods of DNA distribution data analysis based on bromodeoxyuridine/DNA bivariate data.

F Lacombe1, F Belloc, P Bernard, M R Boisseau.   

Abstract

Four published methods of DNA-content histogram analysis (those of Fried, Dean and Jett, simplified Dean, and Fox) were compared using a double labeling of different cell populations. Partially synchronized and asynchronous cell populations were incubated with bromodeoxyuridine (BrdUrd) and then stained with an anti-BrdUrd monoclonal antibody and propidium iodide (PI). The fractions of cells in the G1, S, and G2 + M phases were calculated by each method and compared with those derived from G1, S, and G2 + M areas plotted on BrdUrd/DNA bivariate histograms, taken as the "true" values. This procedure enabled an optimal choice of method for a given cell population.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3378459     DOI： 10.1002/cyto.990090310

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  8 in total

1.  Neuroprotective effects of G-CSF administration in microglia-mediated reactive T cell activation in vitro.

Authors:  Wei Peng
Journal:  Immunol Res       Date:  2017-08       Impact factor: 2.829

2.  Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation.

Authors:  Katie A Matatall; Claudine S Kadmon; Katherine Y King
Journal:  Methods Mol Biol       Date:  2018

3.  Rapamycin blocks production of KSHV/HHV8: insights into the anti-tumor activity of an immunosuppressant drug.

Authors:  Lisa A Nichols; Laura A Adang; Dean H Kedes
Journal:  PLoS One       Date:  2011-01-14       Impact factor: 3.240

4.  Blockage of angiotensin II type I receptor decreases the synthesis of growth factors and induces apoptosis in C6 cultured cells and C6 rat glioma.

Authors:  O Arrieta; P Guevara; E Escobar; R García-Navarrete; B Pineda; J Sotelo
Journal:  Br J Cancer       Date:  2005-04-11       Impact factor: 7.640

5.  The effect of the G1-S transition checkpoint on an age structured cell cycle model.

Authors:  Gary S Chaffey; David J B Lloyd; Anne C Skeldon; Norman F Kirkby
Journal:  PLoS One       Date:  2014-01-09       Impact factor: 3.240

6.  Follistatin-like 1 protects against hypoxia-induced pulmonary hypertension in mice.

Authors:  Wei Zhang; Wang Wang; Jie Liu; Jinna Li; Juan Wang; Yunxia Zhang; Zhifei Zhang; Yafei Liu; Yankun Jin; Jifeng Li; Jie Cao; Chen Wang; Wen Ning; Jun Wang
Journal:  Sci Rep       Date:  2017-03-31       Impact factor: 4.379

7.  Cell cycle-dependent cytotoxicity and induction of apoptosis by liposomal N4-hexadecyl-1-beta-D-arabinofuranosylcytosine.

Authors:  D H Horber; P von Ballmoos; H Schott; R A Schwendener
Journal:  Br J Cancer       Date:  1995-11       Impact factor: 7.640

8.  Rearrangement of Actin Microfilaments in the Development of Olfactory Receptor Cells in Fish.

Authors:  Igor V Klimenkov; Nikolay P Sudakov; Mikhail V Pastukhov; Mikhail M Svinov; Nikolay S Kositsyn
Journal:  Sci Rep       Date:  2018-02-27       Impact factor: 4.379
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.